本文選題：超高效液相色譜法 + 黃連生物堿　； 參考：《中國(guó)藥房》2017年33期

【摘要】：目的:建立同時(shí)測(cè)定連參通淋片中表小檗堿、鹽酸藥根堿、黃連堿、鹽酸巴馬汀和鹽酸小檗堿含量的方法。方法:采用超高效液相色譜法。色譜柱為T(mén)hermo Scientific Syncronis C_(18),流動(dòng)相為乙腈-0.1%磷酸溶液(加三乙胺調(diào)p H至3.0)(30∶70,V/V),流速為0.3 mL/min,檢測(cè)波長(zhǎng)為345 nm,柱溫為25℃,進(jìn)樣量為2μL。結(jié)果:表小檗堿、鹽酸藥根堿、黃連堿、鹽酸巴馬汀和鹽酸小檗堿檢測(cè)進(jìn)樣量線(xiàn)性范圍分別為4.08~163 ng(r=0.999 9)、5.72~229 ng(r=0.999 8)、5.67~227 ng(r=0.999 9)、6.78~271 ng(r=0.999 6)、13.2~526 ng(r=0.999 9);精密度、穩(wěn)定性、重復(fù)性試驗(yàn)的RSD2.0%;加樣回收率分別為96.83%~103.92%(RSD=2.2%,n=9)、97.62%~104.85%(RSD=2.9%,n=9)、99.44%~104.18%(RSD=1.8%,n=9)、98.64%~104.42%(RSD=1.8%,n=9)、98.24%~102.40%(RSD=1.5%,n=9)。結(jié)論:該方法簡(jiǎn)便、靈敏、快速、準(zhǔn)確,適用于連參通淋片中5種黃連生物堿類(lèi)成分含量的同時(shí)測(cè)定。
[Abstract]:Objective: to establish a method for simultaneous determination of berberine, root base, Coptis, palmatine hydrochloride and berberine hydrochloride in Lianshen Tonglin tablets. Methods: ultrahigh performance liquid chromatography was used. The mobile phase was acetonitrile-0.1% phosphoric acid solution (adding triethylamine to adjust pH to 30: 70V / V), the flow rate was 0.3 mL / min, the detection wavelength was 345 nm, the column temperature was 25 鈩，

本文編號(hào)：1782462