本文選題：蘆葦　切入點：蒸汽爆破　出處：《南京理工大學》2016年碩士論文

【摘要】：為探索蘆葦資源化利用——生成低聚木糖的方法,本文研究了水解蘆葦原料制備低聚木糖的工藝流程,其具體內(nèi)容包括蘆葦原料預處理提取木聚糖工藝、爆破蘆葦酶法水解工藝、低聚木糖脫色工藝,以及采用柱層析法分離純化低聚木糖液的研究。蘆葦預處理工藝研究結果表明：堿蒸煮法、酸蒸煮法和蒸汽爆破法均能夠從蘆葦中提取出木聚糖,酸法的木聚糖提取量較少；另外,蒸汽爆破還會爆出少許低聚木糖；蒸汽爆破法中木聚糖的爆出量隨著汽爆壓力增大而增加,且相同壓力下,加堿爆破物料木聚糖爆出量較高；相同的酶解條件下,1.40Mpa加堿爆破的蘆葦物料酶解效果最好,最適合作為蘆葦酶解生產(chǎn)低聚木糖的前處理方式。木聚糖酶水解爆破蘆葦制備低聚木糖的工藝研究結果表明：當加堿爆破蘆葦原料與去離子水比(固液比)為1：10、木聚糖酶添加量為5%(對蘆葦干物質(zhì)量)、pH值4.8、酶解溫度45℃、酶解時間24h時,可獲得較好的酶解效果；水解液中可溶性總糖量達到6.39mg/ml,還原糖含量達到3.51mg/ml；高效液相色譜分析計算酶解液中低聚木糖含量為14.00%(對蘆葦干物質(zhì)量),其主要成分為木二糖和木三糖,含量分別為12.29%和1.71%(對蘆葦干物質(zhì)量)；最佳酶解條件下,低聚木糖中木二糖、木三糖分別在24h、18h達到最大值。對低聚木糖液脫色的研究中得到以下結論：酸析具有一定的脫色效果,在條件pH3.0、50℃脫色30min,脫色率達到45.79%；采用氨水改性后的粉末狀活性炭具有更好的脫色效果,其優(yōu)化的脫色條件為pH值3、脫色溫度70℃、活性炭添加量30%(對固形物)、振蕩頻率為170r/min、脫色時間70min,此時的脫色率達到90.31%；酸析結合活性炭聯(lián)合脫色,活性炭添加量20%(對固形物)的脫色率能達到88.60%。最后,采用顆粒狀活性炭柱層析分離提純脫色后的低聚木糖液,用30%乙醇溶液作為洗脫液,蒸發(fā)濃縮后得到木二糖、木三糖的低聚木糖產(chǎn)品,低聚木糖提取率達到62.9%。
[Abstract]:In order to explore the method of utilizing reeds to produce xylooligosaccharides, the technological process of preparing xylooligosaccharides from Reed raw materials was studied in this paper. The specific contents of this process include extraction of xylan from Reed raw materials and enzymatic hydrolysis of Reed by blasting. Studies on decolorization of xylose oligosaccharides and separation and purification of xylooligosaccharide solution by column chromatography. The results of pretreatment of Reed showed that xylan could be extracted from Reed by alkali cooking, acid cooking and steam explosion. The amount of xylan extracted by acid method is less, in addition, a little xylooligosaccharide is produced by steam blasting, and the amount of xylan explosion increases with the increase of steam explosion pressure, and under the same pressure, the amount of xylan explosion of alkali blasting material is higher. Under the same enzymatic hydrolysis condition, the Reed material with 1.40Mpa added alkali blasting has the best enzymatic hydrolysis effect. The results showed that the ratio of raw material to deionized water (solid / liquid ratio) was 1: 10 when the ratio of raw material to deionized water was 1: 10. Xylanase addition was 5% (pH 4. 8 for Reed dry matter, enzymatic hydrolysis temperature 45 鈩，

本文編號：1695263