本文選題：碳量子點(diǎn)　切入點(diǎn)：蛋白質(zhì)　出處：《遼寧大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：近年來(lái),碳量子點(diǎn)因其卓越的光學(xué)、化學(xué)、生物學(xué)特性,受到了科研人員極大地關(guān)注。相比其他的熒光納米材料,如由重金屬作為重要組成的高熒光性半導(dǎo)體量子點(diǎn),碳量子點(diǎn)具有生物相容性更好,制備方法更簡(jiǎn)單、綠色等特點(diǎn)。本論文通過(guò)微波法處理兩種不同的碳源,成功地制備了兩種碳量子點(diǎn),并通過(guò)多種分析手段對(duì)兩種碳量子點(diǎn)進(jìn)行了表征。與此同時(shí),又對(duì)這兩種碳量子點(diǎn)的性質(zhì)進(jìn)行了深入研究,得到了如下結(jié)論:(1)通過(guò)一步微波法處理變性蛋白質(zhì)(dBSA)制備碳量子點(diǎn),這是一種簡(jiǎn)單、綠色的制備高熒光碳量子點(diǎn)的方法。在這個(gè)體系中,用微波法制備碳量子點(diǎn)節(jié)約了大量的時(shí)間和能量,同時(shí)抑制副反應(yīng)發(fā)生。通過(guò)此方法制備的變性蛋白質(zhì)碳量子點(diǎn)(DPCDs)的熒光量子產(chǎn)率可達(dá)到13.3%。同時(shí),DPCDs具有良好的上轉(zhuǎn)換性質(zhì)。此外,又對(duì)DPCDs的形成機(jī)理進(jìn)行了研究,提出了DPCDs形成所需經(jīng)歷的四個(gè)階段,包括:交聯(lián)、芳構(gòu)化、成核、增長(zhǎng)。另外,制備的DPCDs還表現(xiàn)出了對(duì)Ag~+的選擇性,可以用于檢測(cè)Ag~+。當(dāng)Ag~+濃度為0.1到10.0μM時(shí),淬滅率和Ag~+濃度之間具有一個(gè)R2=0.991的擬合曲線。(2)通過(guò)綠色、簡(jiǎn)單的微波法處理離子液體(ILs,[PAVIm~+][Br-])和乙二胺混合物制備了碳量子點(diǎn)。離子液體基碳量子點(diǎn)(ILCDs)對(duì)Cr(VI)展現(xiàn)了良好的選擇性和靈敏性。當(dāng)Cr(VI)濃度在0.05 10.0μM范圍內(nèi),ILCDs熒光強(qiáng)度和Cr(VI)濃度之間展現(xiàn)了良好的線性關(guān)系,線性方程為:(F0-F)/F0=0.0222×CCr(μM)~+0.0047,(R2=0.994),檢出限為0.015μM(S/N=3)。而世界衛(wèi)生組織(WHO)對(duì)飲用水中Cr(VI)含量規(guī)定為50.0μg L-1(約1.0μM),這個(gè)濃度剛好在ILCDs對(duì)Cr(VI)的檢測(cè)范圍內(nèi)。在實(shí)際樣品中對(duì)Cr(VI)檢測(cè),得到了令人滿意的結(jié)果。此外,ILCDs也可以作為熒光探針檢測(cè)溶液中pH和溫度變化。
[Abstract]:In recent years, carbon quantum dots have attracted great attention due to their excellent optical, chemical and biological properties. Compared with other fluorescent nanomaterials, such as highly fluorescent semiconductor quantum dots composed of heavy metals, Carbon quantum dots have the advantages of better biocompatibility, simpler preparation method and green. In this paper, two kinds of carbon quantum dots were successfully prepared by microwave treatment of two different carbon sources. At the same time, the properties of the two kinds of carbon quantum dots were studied. The conclusion is as follows: 1) carbon quantum dots are prepared by one-step microwave treatment of denatured protein dBSAs. This is a simple, green method for preparing high fluorescent carbon quantum dots. In this system, microwave preparation of carbon quantum dots saves a lot of time and energy. The fluorescence quantum yield of denatured protein carbon quantum dots (DPCDs) obtained by this method can reach 13.3. At the same time, DPCDs have good up-conversion properties. In addition, the formation mechanism of DPCDs is also studied. Four stages of formation of DPCDs were proposed, including crosslinking, aromatization, nucleation and growth. In addition, the prepared DPCDs showed selectivity to Ag ~, and could be used to detect Ag ~-when Ag~ concentration was 0.1 to 10.0 渭 M. There is a fitting curve of R2O0.991 between quenching rate and Ag- concentration. Carbon quantum dots (QDs) were prepared by simple microwave treatment of ionic liquids (ILs), [PAVIm-] [Br-] and ethylenediamine mixtures. Ionic liquid-based carbon quantum dots (CQDs) showed good selectivity and sensitivity to CrVI. The fluorescence intensity of ILCDs was obtained in the range of 0.05 渭 M for CrVI and 10.0 渭 M for ethylene diamine. And CrVI concentrations show a good linear relationship, The linear equation is 0. 0222 脳 CCr1 (渭 M ~ 0. 0047 ~ 0. 007 ~ 0. 07), and the detection limit is 0. 015 渭 M ~ (-1). The World Health Organization (WHO) prescribes that the content of CrVI in drinking water is 50. 0 渭 g L ~ (-1). This concentration is within the detection range of ILCDs for CrVI. In actual samples, CrVI is detected. In addition, the ILCDs can also be used as a fluorescence probe to detect the pH and temperature changes in the solution.
【學(xué)位授予單位】：遼寧大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：O657.3;TB383.1

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