本文選題：金屬有機骨架　切入點：魯米諾　出處：《西南大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：化學(xué)發(fā)光分析法因其無背景散射光干擾、靈敏度高、線性范圍寬、分析速度快及儀器設(shè)備簡單等優(yōu)勢已成為一種重要的分析檢測手段。魯米諾因水溶性好、發(fā)光量子產(chǎn)率較高,是化學(xué)發(fā)光體系中最常用且應(yīng)用最廣泛的化學(xué)發(fā)光試劑之一。魯米諾在水溶液中的發(fā)光速率很慢,常需要加入催化劑,而常用的催化劑大多是金納米顆粒,金屬氧化物納米顆粒等材料。金屬有機骨架化合物(Metal-Organic Frameworks,MOFs),是由金屬離子和有機配體通過配位鍵自組裝形成的多孔有機-無機雜化材料。MOFs綜合了有機和無機的優(yōu)良性質(zhì),具備良好的吸附分離、光學(xué)和催化等性質(zhì),且擁有規(guī)則可調(diào)的孔道結(jié)構(gòu),超高的比表面積,在儲氫、催化及分析檢測等領(lǐng)域得到廣泛應(yīng)用,但MOFs作為催化劑在化學(xué)發(fā)光分析中的應(yīng)用還很少有報道。本文利用兩種具有催化性質(zhì)的MOFs材料,建立了新的化學(xué)發(fā)光分析體系,在無外加氧化劑的條件下,實現(xiàn)了對半胱氨酸、過氧化氫及葡萄糖的靈敏檢測。具體研究內(nèi)容如下:(1)Co-MOFs-魯米諾化學(xué)發(fā)光體系檢測半胱氨酸。我們選擇了由Co~(2+)介導(dǎo)的從含鋅金屬有機凝膠(Zn-MOGs)-晶體轉(zhuǎn)換得到的含鈷金屬有機骨架化合物(Co-MOFs),在無額外添加氧化劑的條件下,直接催化魯米諾化學(xué)發(fā)光。研究發(fā)現(xiàn),與水熱法合成的Co-MOFs(H)及鈷離子相比,由凝膠-結(jié)晶轉(zhuǎn)換制備得到的Co-MOFs的催化活性更強。半胱氨酸因能進一步增強魯米諾-Co-MOFs體系的化學(xué)發(fā)光強度,且在濃度范圍為2μM-10μM內(nèi),半胱氨酸濃度與化學(xué)發(fā)光強度呈良好的線性關(guān)系,據(jù)此,我們構(gòu)建一種簡單靈敏的化學(xué)發(fā)光檢測方法用于半胱氨酸定量檢測,檢測限為0.49μM,所建立的方法能用于復(fù)方氨基酸注射液中半胱氨酸的含量測定。(2)Fe_3O_4/MIL-101(Fe)-魯米諾化學(xué)發(fā)光體系檢測過氧化氫和葡萄糖。利用Fe_3O_4/MIL-101(Fe)復(fù)合材料的協(xié)同催化活性,無需加入氧化劑,Fe_3O_4/MIL-101(Fe)可與溶液中的溶解氧反應(yīng)產(chǎn)生大量的活性氧自由基,活性氧自由基再氧化魯米諾化學(xué)發(fā)光。過氧化氫能增強該Fe_3O_4/MIL-101(Fe)-魯米諾體系的化學(xué)發(fā)光強度,并且葡萄糖在葡萄糖氧化酶的氧化作用下能產(chǎn)生過氧化氫,據(jù)此,該Fe_3O_4/MIL-101(Fe)-魯米諾體系被用于定量檢測過氧化氫和葡萄糖。在最優(yōu)的條件下,檢測過氧化氫和葡萄糖的的線性范圍分別是5nM-150nM和5nM-100nM,檢測限分別是3.7nM和4.9nM。將建立的方法用于醫(yī)用過氧化氫消毒液中過氧化氫及血清中血糖含量的定量分析,獲得了較為滿意的結(jié)果。
[Abstract]:Chemiluminescence analysis has become an important analytical method because of its advantages of no background scattering light interference, high sensitivity, wide linear range, fast analysis speed and simple instrument and equipment. Luminol is one of the most commonly used and widely used chemiluminescence reagents in chemiluminescence systems. Metal oxide nanoparticles. Metal-Organic Framework compounds Metal-Organic frameworks MOFsN, a porous organic-inorganic hybrid material formed by self-assembly of metal ions and organic ligands through coordination bonds, have integrated the excellent properties of organic and inorganic materials. It has good adsorption and separation, optical and catalytic properties, regular and adjustable pore structure, high specific surface area, and has been widely used in hydrogen storage, catalysis and analytical detection, etc. However, the application of MOFs as a catalyst in chemiluminescence analysis is seldom reported. In this paper, a new chemiluminescence analysis system was established by using two kinds of MOFs materials with catalytic properties. Sensitive detection of hydrogen peroxide and glucose-determination of cysteine by the: 1: 1 co-MOFs- luminol chemiluminescence system. We selected cobalt-containing gold from zinc-containing organo-gels Zn-MOGsN-crystal transition mediated by Co~(2. Of the organic skeleton compound Co-MOFsN, without the addition of oxidants, Direct catalysis of luminol chemiluminescence. It was found that compared with Co-MOFsHand cobalt ions synthesized by hydrothermal method, The catalytic activity of Co-MOFs prepared by gel-crystallization conversion is stronger. Cysteine can further enhance the chemiluminescence intensity of Lumino-Co-MOFs system, and in the concentration range of 2 渭 M-10 渭 M, cysteine can further enhance the chemiluminescence intensity of Lumino-Co-MOFs system. There is a good linear relationship between the concentration of cysteine and the intensity of chemiluminescence. Therefore, a simple and sensitive chemiluminescence method is developed for the quantitative detection of cysteine. The detection limit is 0.49 渭 M. the established method can be used for the determination of cysteine in compound amino acid injection, and can be used to determine the synergistic catalytic activity of Fe3O4 / MIL-101FeN-Rumino chemiluminescence system for the determination of hydrogen peroxide and glucose. No need to add oxidant Fe3O-1) can produce a large amount of reactive oxygen radicals, which can be reoxidized by reactive oxygen radicals to luminol chemiluminescence. Hydrogen peroxide can enhance the chemiluminescence intensity of the Fe3O4 / MIL-101FE-Lumino system. And glucose can be oxidized by glucose oxidase to produce hydrogen peroxide, so this Fe3O / MIL-101 Fetio-Rumino system is used to quantitatively detect hydrogen peroxide and glucose. The linear range for the detection of hydrogen peroxide and glucose was 5nM-150nM and 5nM-100nM, respectively, and the detection limits were 3.7nM and 4.9nM. the established method was applied to the quantitative analysis of hydrogen peroxide in medical hydrogen peroxide disinfectant solution and blood sugar content in serum. Satisfactory results have been obtained.
【學(xué)位授予單位】：西南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：O657.3;R446

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本文編號：1595309