基于羥基自由基高級氧化快速殺滅錐狀斯氏藻孢囊的研究
發(fā)布時間:2018-07-29 15:57
【摘要】:隨著船舶壓載水的轉(zhuǎn)運(yùn),我國面臨嚴(yán)重的外來入侵生物風(fēng)險,其中部分赤潮藻形成的孢囊可成為赤潮的"種源",嚴(yán)重危害近岸海洋環(huán)境,因而快速有效地殺滅赤潮藻孢囊至關(guān)重要。本文利用大氣壓強(qiáng)電離放電高效生成的羥基自由基(·OH),對典型赤潮藻錐狀斯氏藻孢囊進(jìn)行殺滅研究。采用萌發(fā)實驗、SYTOX Green熒光染色法確定·OH殺滅錐狀斯氏藻孢囊的閾值和時間,掃描電子顯微鏡觀察孢囊的形態(tài)變化。結(jié)果表明,·OH殺滅孢囊的CT閾值為0.49mg·min/L,時間為10s,·OH氧化降解孢囊體內(nèi)葉綠素,破壞DNA,抑制萌發(fā),具有其他方法無法比擬的優(yōu)勢。因此,·OH快速殺滅赤潮藻孢囊的新方法,對防控外來入侵生物引發(fā)的海洋赤潮災(zāi)害具有重要的作用。
[Abstract]:Along with the ship ballast water transportation, our country faces the serious alien invasion biological risk, some of the spore sac formed by the red tide algae can become the "provenance" of the red tide, which seriously endangers the coastal marine environment. Therefore, it is very important to kill the cysts of red tide algae quickly and effectively. In this paper, hydroxyl radical (OH), produced by atmospheric pressure ionization discharge was used to kill the cysts of typical red tide algae. The threshold value and time for OH to kill cysts of Staphylococcus pyramidis were determined by SYTOX Green fluorescence staining method. The morphological changes of cysts were observed by scanning electron microscope (SEM). The results showed that the CT threshold of OH to kill cysts was 0.49mg min / L, and the time was 10 s. OH oxidized the chlorophyll, destroyed the DNA and inhibited the germination of cysts, which was superior to other methods. Therefore, a new method of killing algal cysts rapidly by OH plays an important role in the prevention and control of marine red tide disaster caused by alien invading organisms.
【作者單位】: 廈門大學(xué)福建省海洋生物資源開發(fā)利用協(xié)同創(chuàng)新中心濱海濕地生態(tài)系統(tǒng)教育部重點實驗室環(huán)境與生態(tài)學(xué)院;廈門大學(xué)海洋與地球?qū)W院;
【基金】:國家科技支撐計劃項目(2013BAC06B01,2013BAC06B02) 國家重大科研儀器研制項目(61427804) 科技部創(chuàng)新人才推進(jìn)計劃重點領(lǐng)域創(chuàng)新團(tuán)隊(2015RA4008)
【分類號】:U698.7
本文編號:2153191
[Abstract]:Along with the ship ballast water transportation, our country faces the serious alien invasion biological risk, some of the spore sac formed by the red tide algae can become the "provenance" of the red tide, which seriously endangers the coastal marine environment. Therefore, it is very important to kill the cysts of red tide algae quickly and effectively. In this paper, hydroxyl radical (OH), produced by atmospheric pressure ionization discharge was used to kill the cysts of typical red tide algae. The threshold value and time for OH to kill cysts of Staphylococcus pyramidis were determined by SYTOX Green fluorescence staining method. The morphological changes of cysts were observed by scanning electron microscope (SEM). The results showed that the CT threshold of OH to kill cysts was 0.49mg min / L, and the time was 10 s. OH oxidized the chlorophyll, destroyed the DNA and inhibited the germination of cysts, which was superior to other methods. Therefore, a new method of killing algal cysts rapidly by OH plays an important role in the prevention and control of marine red tide disaster caused by alien invading organisms.
【作者單位】: 廈門大學(xué)福建省海洋生物資源開發(fā)利用協(xié)同創(chuàng)新中心濱海濕地生態(tài)系統(tǒng)教育部重點實驗室環(huán)境與生態(tài)學(xué)院;廈門大學(xué)海洋與地球?qū)W院;
【基金】:國家科技支撐計劃項目(2013BAC06B01,2013BAC06B02) 國家重大科研儀器研制項目(61427804) 科技部創(chuàng)新人才推進(jìn)計劃重點領(lǐng)域創(chuàng)新團(tuán)隊(2015RA4008)
【分類號】:U698.7
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