本文選題：法醫(yī)病理學　切入點：VEGF　出處：《中國法醫(yī)學雜志》2008年01期 　論文類型：期刊論文

【摘要】：目的觀察心肌中VEGF在死后一定時間的穩(wěn)定性及表達的變化規(guī)律,評價其對死后診斷機體早期心肌缺血的作用。方法用冠狀動脈結扎法建立家兔急性心肌缺血模型,應用SP法染色、圖像分析和統(tǒng)計學處理系統(tǒng),檢測缺血心肌細胞內(nèi)VEGF陽性表達面積和強度,并對VEGF在死后不同時間的穩(wěn)定性進行比較。結果VEGF在缺血心肌組織中,見灶性或成片心肌細胞強陽性表達,部分細胞呈陰性或弱陽性表達,血管內(nèi)皮及平滑肌細胞表達弱陽性,間質(zhì)表達陰性;在4℃放置,隨時間延長,缺血心肌組織VEGF陽性表達有減弱趨勢,至10d時缺血心肌組織仍可見VEGF在少許心肌細胞胞漿內(nèi)呈弱陽性表達。在4℃放置10d以內(nèi),缺血心肌和正常心肌標本之間VEGF陽性表達具有極顯著性差異。結論VEGF能耐受一定程度自溶及腐敗的影響,其免疫組化檢測對于判斷死后4℃放置10d以內(nèi)的尸體是否出現(xiàn)過心肌缺血具有一定意義。
[Abstract]:Objective to observe the stability and expression of VEGF in myocardium at a certain time after death and to evaluate its role in the diagnosis of early myocardial ischemia. Methods A rabbit model of acute myocardial ischemia was established by coronary artery ligation. The area and intensity of VEGF positive expression in ischemic cardiomyocytes were detected by SP staining, image analysis and statistical processing system. The stability of VEGF at different time after death was compared. There was strong positive expression in focal or adult cardiomyocytes, negative or weak positive expression in some cells, weak positive expression in vascular endothelial and smooth muscle cells and negative expression in interstitial tissue. The positive expression of VEGF in ischemic myocardium tended to weaken, and VEGF was still weakly expressed in the cytoplasm of a few myocardial cells at 10 days after ischemia, and within 10 days at 4 鈩，

本文編號：1608867