【摘要】：目的:探討瘢痕灸預(yù)處理肝俞及足三里穴對(duì)原發(fā)性肝癌大鼠Wnt信號(hào)傳導(dǎo)通路相關(guān)基因表達(dá)的影響。方法:健康雄性Wistar大鼠48只隨機(jī)分為空白組、模型組、預(yù)處理肝俞組和預(yù)處理足三里組,每組12只。預(yù)處理肝俞組和預(yù)處理足三里組均提前15 d施行瘢痕灸后,與模型組一同采用腹腔注射二乙基亞硝胺溶液(50mg/kg)建立肝癌模型。采用ELISA法檢測(cè)血清中甲胎蛋白(alpha fetoprotein,AFP)、γ-谷胺酰轉(zhuǎn)肽酶(Glutamyl transpeptidase,γ-GT)和巖藻糖苷酶(fucosidase,AFU)的表達(dá)量,RT-PCR法檢測(cè)Wnt信號(hào)傳導(dǎo)通路中Wnt3amRNA、Wnt10bmRNA的表達(dá)量,并觀察大鼠肝臟組織形態(tài)學(xué)變化。結(jié)果:與模型組比較,預(yù)處理肝俞組、預(yù)處理足三里組的大鼠AFP、AFU、γ-GT和Wnt3amRNA、Wnt10bmRNA的表達(dá)量均降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05);與預(yù)處理肝俞組比較,預(yù)處理足三里組各指標(biāo)表達(dá)量均顯著升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。HE染色表明模型組腫瘤病變?cè)钜研纬?鏡下可觀察癌變細(xì)胞已深染,形態(tài)不規(guī)則,細(xì)胞核異常,增生結(jié)節(jié)較多;預(yù)處理足三里和預(yù)處理肝俞組兩組癌變細(xì)胞數(shù)量較少,可見較少的結(jié)節(jié)形成。結(jié)論:瘢痕灸預(yù)處理肝俞和足三里穴均可以降低原發(fā)性肝癌大鼠血清中AFP、AFU、γ-GT和Wnt信號(hào)傳導(dǎo)通路中Wnt3amRNA、Wnt10bmRNA的表達(dá)量,且瘢痕灸肝俞穴的預(yù)防作用更為顯著。
[Abstract]:Aim: to investigate the effects of moxibustion with scarring on the expression of Wnt signal transduction pathway-related genes in rats with primary liver cancer (PHC) after pretreatment at Gan Shu and Zusanli points. Methods: forty-eight healthy male Wistar rats were randomly divided into blank group, model group, Gan Shu pretreatment group and Zusanli pretreatment group, with 12 rats in each group. Liver cancer model was established by intraperitoneal injection of diethylnitrosamine (50mg/kg) 15 days ahead of schedule in pre-treated Gansu group and Zusanli group. The expression of alpha-fetoprotein (alpha fetoprotein,AFP), 緯-glutamyl transpeptidase (Glutamyl transpeptidase, 緯-GT) and fucosidase (fucosidase,AFU) in serum was detected by ELISA, and the expression of Wnt3amRNA,Wnt10bmRNA in Wnt signal transduction pathway was detected by RT-PCR method. The morphological changes of rat liver were observed. Results: compared with the model group, the expression of AFP,AFU, 緯-GT and Wnt3amRNA,Wnt10bmRNA in pretreated Gan Shu group and Zusanli group decreased significantly (P0.05). Compared with the pretreated Gan Shu group, the expression of each index in the pretreated Zusanli group was significantly higher than that in the pretreated Gan Shu group (P0.05). HE staining showed that the tumor lesions had been formed in the model group, and the canceration cells were deeply stained under the microscope. Irregular shape, abnormal nucleus and more hyperplastic nodules; The number of canceration cells in Zusanli pretreated group and Gan Shu pretreated group were fewer and less nodular formation was observed. Conclusion: both Gansu and Zusanli acupoints pre-treated with scar moxibustion can decrease the expression of AFP,AFU, 緯-GT and Wnt3amRNA,Wnt10bmRNA in the signal transduction pathway of Wnt in the serum of rats with primary liver cancer, and the preventive effect of moxibustion with scar on Gan Shu point is more obvious.
【作者單位】： 遼寧中醫(yī)藥大學(xué)針灸推拿學(xué)院;沈陽(yáng)市中醫(yī)院;遼寧中醫(yī)藥大學(xué)第一臨床學(xué)院;
【基金】：遼寧省自然科學(xué)基金項(xiàng)目(2014020047)
【分類號(hào)】：R245.8

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