發(fā)布時(shí)間：2019-03-06 16:11

【摘要】：目的:膝關(guān)節(jié)骨性關(guān)節(jié)炎(KOA)是最常見的退行性骨關(guān)節(jié)疾病,熏洗療法在KOA的治療中越來越受到關(guān)注,但傳統(tǒng)熏洗法存在空間開放,時(shí)間、溫度不可控等缺點(diǎn),針對(duì)以上問題,我科研發(fā)膝關(guān)節(jié)肢段封閉式熏洗儀,并使其聯(lián)合獨(dú)活寄生湯熏洗治療自然退變KOA的老年食蟹猴,檢測(cè)其對(duì)老年食蟹猴KOA關(guān)節(jié)軟骨中Notch1、MMP-13、COL-Ⅱ水平表達(dá)的影響,探討膝關(guān)節(jié)肢段封閉式熏洗儀對(duì)KOA的治療效果,以及獨(dú)活寄生湯熏洗治療KOA的治療效果及作用機(jī)制。方法:第一部分,進(jìn)行膝關(guān)節(jié)肢段封閉式熏洗儀的設(shè)計(jì)與研發(fā),并生產(chǎn)出樣機(jī),申請(qǐng)專利。第二部分,選取自然退變患有KOA的老年食蟹猴12只,隨機(jī)分為實(shí)驗(yàn)組、對(duì)照組、空白對(duì)照組,每組各4只。實(shí)驗(yàn)組用膝關(guān)節(jié)肢段封閉式熏洗聯(lián)合獨(dú)活寄生湯熏洗,對(duì)照組運(yùn)用傳統(tǒng)熏洗法聯(lián)合獨(dú)活寄生湯熏洗,空白組用生理鹽水進(jìn)行傳統(tǒng)熏洗處理。干預(yù)8周后處死動(dòng)物,制作老年食蟹猴膝關(guān)節(jié)退變軟骨細(xì)胞病理切片,鏡下觀察其病變后行mankin評(píng)分,最后對(duì)退變軟骨細(xì)胞Notch1、MMP-13、COL-Ⅱ免疫組化染色,觀察其表達(dá)。結(jié)果:(1)在繼承傳統(tǒng)熏洗法的基礎(chǔ)上結(jié)合現(xiàn)代最新的生物科學(xué)技術(shù)完成膝關(guān)節(jié)肢段式熏洗儀的初步設(shè)計(jì)以及實(shí)體模型的制造,已生產(chǎn)出一臺(tái)樣機(jī),申報(bào)了國(guó)家專利,專利號(hào)(ZL 2013 2 0763945.X)。(2)HE染色觀察中,實(shí)驗(yàn)組、對(duì)照組Mankin評(píng)分均值低于空白對(duì)照組,組間比較差異具有統(tǒng)計(jì)學(xué)意義(P0.05),實(shí)驗(yàn)組Mankin評(píng)分均值低于對(duì)照組,組間比較差異具有統(tǒng)計(jì)學(xué)意義(P0.05)。在免疫組化觀察中,實(shí)驗(yàn)組、對(duì)照組Notch1、MMP-13的表達(dá)均較空白對(duì)照組明顯降低,組間比較差異有統(tǒng)計(jì)學(xué)意義(P0.05);實(shí)驗(yàn)組Notch1、MMP-13的表達(dá)率較對(duì)照組顯著降低,組間比較差異有統(tǒng)計(jì)學(xué)意義(P0.05);實(shí)驗(yàn)組、對(duì)照組COL-Ⅱ的陽性表達(dá)率均較空白對(duì)照組明顯升高,組間比較差異有統(tǒng)計(jì)學(xué)意義(P0.05);實(shí)驗(yàn)組COL-Ⅱ陽性表達(dá)率較對(duì)照組顯著升高,組間比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論:(1)膝關(guān)節(jié)肢段封閉式熏洗儀較傳統(tǒng)熏洗具有恒溫、封閉、時(shí)間可控等優(yōu)點(diǎn);(2)獨(dú)活寄生湯加減外用熏洗能調(diào)節(jié)膝關(guān)節(jié)軟骨中Notch1、MMP-13、COL-Ⅱ的水平,抑制關(guān)節(jié)軟骨的破壞,有效延緩膝關(guān)節(jié)退變,達(dá)到其治療作用,膝關(guān)節(jié)肢段封閉式熏洗儀較傳統(tǒng)熏洗法對(duì)KOA具有更好的治療效果。
[Abstract]:Objective: (KOA) is the most common degenerative osteoarthritis of the knee joint. Fumigation and washing therapy has attracted more and more attention in the treatment of KOA. However, the traditional fumigation method has many disadvantages, such as open space, uncontrollable time and temperature, and so on. In order to solve the above problems, we have studied the closed fumigation and washing apparatus of knee joint segment, and made it combined with Duhuoshi decoction to treat the elderly crab-eating monkeys with naturally degenerated KOA, and detected the Notch1,MMP-13, in the articular cartilage of the aged crab-eating monkeys KOA. The effect of COL- 鈪，

本文編號(hào)：2435682