發(fā)布時間：2019-02-28 16:09

【摘要】：研究發(fā)現(xiàn)五味甘露藥浴散加減方藥浴有良好的抗炎活性,可以明顯改善關節(jié)炎癥程度,對類風濕性關節(jié)炎具有一定的治療作用,推測其可能與調(diào)控滑膜細胞MAPK通路有關。該實驗通過建立佐劑關節(jié)炎大鼠(AA)模型,進一步探討其調(diào)控滑膜細胞MAPK通路的機制。采用酶聯(lián)免疫法測定給藥2周AA大鼠血清中TNF-α的水平,免疫組化法測定給藥4周踝關節(jié)滑膜組織蛋白激酶ERK1/2,p38的含量,采用熒光定量PCR(RT-PCR)法檢測膝關節(jié)滑膜組織中JNK1,ERK1,p38的mRNA表達。結果顯示,與模型組比較,給藥2周,可明顯降低AA大鼠血清中TNF-α的水平(P0.05);給藥4周,降低踝關節(jié)滑膜組織中p38,ERK1/2的蛋白表達(P0.05或P0.01),降低膝關節(jié)滑膜組織JNK1,p38,ERK1的基因表達(P0.05或P0.01)。綜上可見,五味甘露藥浴散加減方有效治療類風濕關節(jié)炎的作用機制可能與其降低血清中TNF-α的水平和滑膜組織中p38,ERK1/2的表達以及JNK1,p38,ERK1的基因表達,調(diào)控MAPK通路有關。
[Abstract]:It was found that the combination of Wuwei mannitol powder and antiinflammatory drug bath had good anti-inflammatory activity and could obviously improve the degree of joint inflammation and had a certain therapeutic effect on rheumatoid arthritis, which might be related to the regulation of MAPK pathway in synovial cells. In this study, the (AA) model of adjuvant arthritis rats was established to further explore the mechanism of its regulation of MAPK pathway in synovial cells. The serum level of TNF- 偽 was measured by enzyme linked immunosorbent assay (Elisa) in AA rats for 2 weeks, and the protein kinase ERK1/2,p38 in synovial tissue of ankle joint was measured by immunohistochemical method at 4 weeks after administration. Fluorescence quantitative PCR (RT-PCR) was used to detect the expression of JNK1,ERK1,p38 mRNA in synovial tissue of knee joint. The results showed that compared with the model group, the level of TNF- 偽 in serum of AA rats was significantly decreased after administration for 2 weeks (P0.05). After 4 weeks of administration, the protein expression of p38 and ERK1 / 2 in synovial tissue of ankle joint was decreased (P0.05 or P0.01), and the gene expression of JNK1,p38,ERK1 in synovial tissue of knee joint was decreased (P0.05 or P0.01). In conclusion, we can see that the mechanism of effective treatment of rheumatoid arthritis by Wuwei mannitol bath powder Jiajianfang may be related to its reduction of serum TNF- 偽 level, expression of p38, ERK 1 and 2 in synovial tissue, and gene expression of JNK1,p38,ERK1, which may be related to the reduction of serum level of ERK1 偽 and ERK1 偽 gene expression in synovial tissue. The regulation of MAPK pathway is related.
【作者單位】： 成都中醫(yī)藥大學;
【基金】：國家自然科學基金地區(qū)科學基金項目(81260672) 四川省科技廳省青年科技創(chuàng)新研究團隊專項(2014TD0007)
【分類號】：R29

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