【摘要】：研究發(fā)現(xiàn)五味甘露藥浴散加減方藥浴有良好的抗炎活性,可以明顯改善關(guān)節(jié)炎癥程度,對類風(fēng)濕性關(guān)節(jié)炎具有一定的治療作用,推測其可能與調(diào)控滑膜細(xì)胞MAPK通路有關(guān)。該實驗通過建立佐劑關(guān)節(jié)炎大鼠(AA)模型,進(jìn)一步探討其調(diào)控滑膜細(xì)胞MAPK通路的機(jī)制。采用酶聯(lián)免疫法測定給藥2周AA大鼠血清中TNF-α的水平,免疫組化法測定給藥4周踝關(guān)節(jié)滑膜組織蛋白激酶ERK1/2,p38的含量,采用熒光定量PCR(RT-PCR)法檢測膝關(guān)節(jié)滑膜組織中JNK1,ERK1,p38的mRNA表達(dá)。結(jié)果顯示,與模型組比較,給藥2周,可明顯降低AA大鼠血清中TNF-α的水平(P0.05);給藥4周,降低踝關(guān)節(jié)滑膜組織中p38,ERK1/2的蛋白表達(dá)(P0.05或P0.01),降低膝關(guān)節(jié)滑膜組織JNK1,p38,ERK1的基因表達(dá)(P0.05或P0.01)。綜上可見,五味甘露藥浴散加減方有效治療類風(fēng)濕關(guān)節(jié)炎的作用機(jī)制可能與其降低血清中TNF-α的水平和滑膜組織中p38,ERK1/2的表達(dá)以及JNK1,p38,ERK1的基因表達(dá),調(diào)控MAPK通路有關(guān)。
[Abstract]:It was found that the combination of Wuwei mannitol powder and antiinflammatory drug bath had good anti-inflammatory activity and could obviously improve the degree of joint inflammation and had a certain therapeutic effect on rheumatoid arthritis, which might be related to the regulation of MAPK pathway in synovial cells. In this study, the (AA) model of adjuvant arthritis rats was established to further explore the mechanism of its regulation of MAPK pathway in synovial cells. The serum level of TNF- 偽 was measured by enzyme linked immunosorbent assay (Elisa) in AA rats for 2 weeks, and the protein kinase ERK1/2,p38 in synovial tissue of ankle joint was measured by immunohistochemical method at 4 weeks after administration. Fluorescence quantitative PCR (RT-PCR) was used to detect the expression of JNK1,ERK1,p38 mRNA in synovial tissue of knee joint. The results showed that compared with the model group, the level of TNF- 偽 in serum of AA rats was significantly decreased after administration for 2 weeks (P0.05). After 4 weeks of administration, the protein expression of p38 and ERK1 / 2 in synovial tissue of ankle joint was decreased (P0.05 or P0.01), and the gene expression of JNK1,p38,ERK1 in synovial tissue of knee joint was decreased (P0.05 or P0.01). In conclusion, we can see that the mechanism of effective treatment of rheumatoid arthritis by Wuwei mannitol bath powder Jiajianfang may be related to its reduction of serum TNF- 偽 level, expression of p38, ERK 1 and 2 in synovial tissue, and gene expression of JNK1,p38,ERK1, which may be related to the reduction of serum level of ERK1 偽 and ERK1 偽 gene expression in synovial tissue. The regulation of MAPK pathway is related.
【作者單位】： 成都中醫(yī)藥大學(xué);
【基金】：國家自然科學(xué)基金地區(qū)科學(xué)基金項目(81260672) 四川省科技廳省青年科技創(chuàng)新研究團(tuán)隊專項(2014TD0007)
【分類號】：R29

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 李運(yùn)馥;;瘺管散加拔火罐治療瘺管10例體會[J];中級醫(yī)刊;1988年12期

2 吳文萍;王苓散加味治療哮喘病36例[J];江西中醫(yī)藥;1995年06期

3 陶學(xué)煦,龔國j,陳元,朱明德;烏甘散加減治療潰瘍病100例的臨床分析[J];上海中醫(yī)藥雜志;1960年06期

4 李德勝,胡翠芳;白枳散加痢特靈治療消化性潰瘍50例[J];湖北中醫(yī)雜志;1994年04期

5 張敏榮;中藥瘺管散加拔火罐治療瘺管病10例體會[J];云南中醫(yī)中藥雜志;2005年05期

6 李學(xué)文;劉廷勝;;玉泉散加味治療2型糖尿病45例[J];實用中醫(yī)內(nèi)科雜志;2007年07期

7 孫永安;鞏提散加味治療水疝[J];四川中醫(yī);1984年05期

8 李貴芬;;銀連清熱散加減治療小兒急性上呼吸道感染100例[J];河南中醫(yī);2013年02期

9 尚小平;劉春社;;六一散加味治愈急性重型肝炎1例[J];中國鄉(xiāng)村醫(yī)藥;2006年08期

10 劉洪榮;;中藥烏芍散加味治療十二指腸潰瘍60例臨床療效觀察[J];時珍國醫(yī)國藥;2006年08期

相關(guān)碩士學(xué)位論文 前3條

1 陳怡萌;回藥乳香應(yīng)痛散加減方治療糖尿病周圍神經(jīng)病變的臨床觀察與研究[D];寧夏醫(yī)科大學(xué);2015年

2 李偉鏘;蒼苓散加味對濕熱型輪狀病毒腸炎的療效觀察及干擾素-γ的影響[D];廣州中醫(yī)藥大學(xué);2015年

3 蔣鴻耀;結(jié)核散加化療治療肺結(jié)核的臨床與實驗研究[D];山東中醫(yī)藥大學(xué);2001年

，

本文編號：2431951