【摘要】：【目的】探討電針對大腦中動脈閉塞(MCAO)缺血再灌注(I/R)大鼠神經(jīng)功能的影響及潛在機制,為應(yīng)用電針治療急性腦梗死提供實驗依據(jù)。【方法】將54只雄性SPF級SD大鼠采用線栓法復(fù)制MCAO模型,按照隨機數(shù)字表法分為假手術(shù)組、腦梗死組、電針組,每組18只大鼠。假手術(shù)組僅做手術(shù)創(chuàng)傷,腦梗死組不予任何干預(yù),電針組予電針針刺百會穴、大椎穴,每天1次,每次30 min。各時間點(第1、7、14天)采用神經(jīng)功能缺損評分量表評定神經(jīng)功能,蛋白免疫印跡法(Western blot)檢測病灶側(cè)海馬區(qū)勿動蛋白-A(Nogo-A)、Ras同源基因家族成員A(RhoA)、溶血磷脂酸(LPA)蛋白的表達�！窘Y(jié)果】第1、7、14天腦梗死組神經(jīng)功能缺損積分、海馬區(qū)Nogo-A及LPA蛋白的表達均顯著高于假手術(shù)組(P0.05),第7、14天腦梗死組病灶側(cè)海馬區(qū)RhoA蛋白的表達顯著高于假手術(shù)組(P0.05);電針組第1、7、14天神經(jīng)功能缺損積分、病灶側(cè)海馬區(qū)RhoA、Nogo-A及LPA蛋白的表達均顯著低于同時間點腦梗死組(P0.05)�！窘Y(jié)論】電針可改善大鼠腦梗死后神經(jīng)功能,其機制可能與電針抑制大鼠海馬區(qū)RhoA通路相關(guān)蛋白的表達密切相關(guān)。
[Abstract]:[objective] to investigate the effects of electroacupuncture on the neural function of middle cerebral artery occlusion (MCAO) rats with (MCAO) ischemia-reperfusion (I / R) and its potential mechanism. [methods] 54 male SPF grade SD rats were randomly divided into sham operation group, cerebral infarction group and electroacupuncture group with 18 rats in each group according to the method of random numerical table, and 54 male SD rats with acute cerebral infarction were divided into sham operation group, cerebral infarction group and electro acupuncture group with 18 rats in each group. Sham operation group only do operation trauma, cerebral infarction group without any intervention, electroacupuncture group to acupuncture Baihui point, Dazhui point, once a day, 30 min. each time. At each time point (1,7,14 days), the neurologic function was assessed by the nerve function defect scale, and the A (RhoA), (A (RhoA), a member of the Nogo-A), Ras homologue gene family) was detected by protein immunoblotting (Western blot) in the focal side of the hippocampus. [results] the expression of lysophosphatidic acid (LPA) protein in cerebral infarction group was significantly higher than that in sham operation group on day 1,7,14 (P0.05), and the expression of Nogo-A and LPA protein in hippocampus of cerebral infarction group was significantly higher than that in sham operation group (P0.05). On the 7th and 14th day, the expression of RhoA protein in cerebral infarction group was significantly higher than that in sham operation group (P0.05). On the 1st, 7th and 14th day, the nerve function defect score, the expression of RhoA,Nogo-A and LPA protein in the hippocampus of the acupuncture group were significantly lower than those in the cerebral infarction group at the same time point (P0.05). [conclusion] electroacupuncture can improve the nerve function after cerebral infarction in rats. The mechanism may be related to the inhibitory effect of electroacupuncture on the expression of RhoA pathway-related proteins in hippocampus of rats.
【作者單位】： 廣東省佛山市中醫(yī)院;南方醫(yī)科大學(xué)南方醫(yī)院;
【基金】：國家自然科學(xué)基金課題資助項目(編號:81473470,81072947) 廣東省自然科學(xué)基金重點項目(編號:8152800007000001,2014A030311033)
【分類號】：R245.97

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