【摘要】：【目的】探討電針對大腦中動(dòng)脈閉塞(MCAO)缺血再灌注(I/R)大鼠神經(jīng)功能的影響及潛在機(jī)制,為應(yīng)用電針治療急性腦梗死提供實(shí)驗(yàn)依據(jù)�！痉椒ā繉�54只雄性SPF級SD大鼠采用線栓法復(fù)制MCAO模型,按照隨機(jī)數(shù)字表法分為假手術(shù)組、腦梗死組、電針組,每組18只大鼠。假手術(shù)組僅做手術(shù)創(chuàng)傷,腦梗死組不予任何干預(yù),電針組予電針針刺百會(huì)穴、大椎穴,每天1次,每次30 min。各時(shí)間點(diǎn)(第1、7、14天)采用神經(jīng)功能缺損評分量表評定神經(jīng)功能,蛋白免疫印跡法(Western blot)檢測病灶側(cè)海馬區(qū)勿動(dòng)蛋白-A(Nogo-A)、Ras同源基因家族成員A(RhoA)、溶血磷脂酸(LPA)蛋白的表達(dá)。【結(jié)果】第1、7、14天腦梗死組神經(jīng)功能缺損積分、海馬區(qū)Nogo-A及LPA蛋白的表達(dá)均顯著高于假手術(shù)組(P0.05),第7、14天腦梗死組病灶側(cè)海馬區(qū)RhoA蛋白的表達(dá)顯著高于假手術(shù)組(P0.05);電針組第1、7、14天神經(jīng)功能缺損積分、病灶側(cè)海馬區(qū)RhoA、Nogo-A及LPA蛋白的表達(dá)均顯著低于同時(shí)間點(diǎn)腦梗死組(P0.05)�！窘Y(jié)論】電針可改善大鼠腦梗死后神經(jīng)功能,其機(jī)制可能與電針抑制大鼠海馬區(qū)RhoA通路相關(guān)蛋白的表達(dá)密切相關(guān)。
[Abstract]:[objective] to investigate the effects of electroacupuncture on the neural function of middle cerebral artery occlusion (MCAO) rats with (MCAO) ischemia-reperfusion (I / R) and its potential mechanism. [methods] 54 male SPF grade SD rats were randomly divided into sham operation group, cerebral infarction group and electroacupuncture group with 18 rats in each group according to the method of random numerical table, and 54 male SD rats with acute cerebral infarction were divided into sham operation group, cerebral infarction group and electro acupuncture group with 18 rats in each group. Sham operation group only do operation trauma, cerebral infarction group without any intervention, electroacupuncture group to acupuncture Baihui point, Dazhui point, once a day, 30 min. each time. At each time point (1,7,14 days), the neurologic function was assessed by the nerve function defect scale, and the A (RhoA), (A (RhoA), a member of the Nogo-A), Ras homologue gene family) was detected by protein immunoblotting (Western blot) in the focal side of the hippocampus. [results] the expression of lysophosphatidic acid (LPA) protein in cerebral infarction group was significantly higher than that in sham operation group on day 1,7,14 (P0.05), and the expression of Nogo-A and LPA protein in hippocampus of cerebral infarction group was significantly higher than that in sham operation group (P0.05). On the 7th and 14th day, the expression of RhoA protein in cerebral infarction group was significantly higher than that in sham operation group (P0.05). On the 1st, 7th and 14th day, the nerve function defect score, the expression of RhoA,Nogo-A and LPA protein in the hippocampus of the acupuncture group were significantly lower than those in the cerebral infarction group at the same time point (P0.05). [conclusion] electroacupuncture can improve the nerve function after cerebral infarction in rats. The mechanism may be related to the inhibitory effect of electroacupuncture on the expression of RhoA pathway-related proteins in hippocampus of rats.
【作者單位】： 廣東省佛山市中醫(yī)院;南方醫(yī)科大學(xué)南方醫(yī)院;
【基金】：國家自然科學(xué)基金課題資助項(xiàng)目(編號:81473470,81072947) 廣東省自然科學(xué)基金重點(diǎn)項(xiàng)目(編號:8152800007000001,2014A030311033)
【分類號】：R245.97

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陳同度,張昌穎;素食大鼠的貧血現(xiàn)象[J];營養(yǎng)學(xué)報(bào);1957年04期

2 陳偉強(qiáng);趙善廣;;自制注射用大鼠固定裝置[J];上海實(shí)驗(yàn)動(dòng)物科學(xué);1992年04期

3 肖柳英,林培英,馮昭明,張丹;不同周齡的SD大鼠生理、生化及體重的正常值測定[J];中藥新藥與臨床藥理;1996年03期

4 李淑云;簡易大鼠灌胃器的制作[J];錦州醫(yī)學(xué)院學(xué)報(bào);2001年04期

5 楊明智,陳積圣;一種大鼠抓取與固定的新工具介紹[J];上海實(shí)驗(yàn)動(dòng)物科學(xué);2001年03期

6 戴英,陸群;復(fù)方H_(505)對Wistar大鼠外周血的血液流變學(xué)指標(biāo)的影響[J];中國血液流變學(xué)雜志;2001年01期

7 韋應(yīng)波,孫喜慶,曹新生,姚永杰,馮岱雅,楊長斌;+Gz暴露時(shí)間對大鼠記憶功能和行為的影響[J];航天醫(yī)學(xué)與醫(yī)學(xué)工程;2003年01期

8 呂學(xué)軍,郭俊生,李敏,周利梅,張永娟;暈船大鼠體內(nèi)鐵含量的變化[J];中國職業(yè)醫(yī)學(xué);2003年04期

9 湯仁仙,王迎偉,王慧,周峰;201A中藥合劑對大鼠抗腎小球基底膜腎炎病變的影響[J];徐州醫(yī)學(xué)院學(xué)報(bào);2003年06期

10 孫同柱,付小兵,翁立新,梁雪梅,陳偉;介紹一種簡易的大鼠保定方法[J];上海實(shí)驗(yàn)動(dòng)物科學(xué);2004年01期

相關(guān)會(huì)議論文 前10條

1 尹音;孫振宇;胡敏;李冬霞;;持續(xù)性高正加速度對大鼠顳頜關(guān)節(jié)損傷的作用[A];第八屆全國顳下頜關(guān)節(jié)病學(xué)及（牙合）學(xué)大會(huì)論文匯編[C];2011年

2 祝~=驤;iJ梊霞;洃克琴;崔素英;文允摪;，

本文編號：2431976