隔藥餅灸對FD肝郁脾虛模型大鼠胃排空率及下丘腦單胺類神經(jīng)遞質(zhì)的影響
發(fā)布時間：2019-01-29 04:49
【摘要】：目的:觀察隔藥餅灸對功能性消化不良(FD)肝郁脾虛模型大鼠胃排空率及下丘腦五羥色胺(5-HT)、多巴胺(DA)、去甲腎上腺素(NE)的影響,探討隔藥餅灸治療FD可能的作用機制。方法:將50只SD大鼠按照隨機數(shù)字表法隨機分為5組,每組10只,分別為空白組、模型組、隔藥餅灸組、艾炷灸組和逍遙散組。空白組予正常飼養(yǎng),其余4組采用復(fù)合病因造模法(慢性束縛應(yīng)激+過度疲勞+飲食失節(jié)+夾尾+搖晃)造模,連續(xù)21d;再按分組施加被試因素,分別進(jìn)行隔藥餅灸、艾炷灸和逍遙散灌胃治療14d。隔藥餅灸組、艾炷灸組施灸穴位均分為I組(“肝俞”“脾俞”“胃俞”)和II組(“章門”“期門”“中脘”),兩組穴位隔日交替,每穴連續(xù)灸4-5壯(約30min);逍遙散組按1ml/100g灌服逍遙散水煎劑。治療結(jié)束后,通過觀察大鼠胃腸對營養(yǎng)性半固體糊的排空情況計算大鼠的胃排空率,采用高效液相色譜-熒光法檢測大鼠下丘腦中5-HT、DA及NE的含量。結(jié)果:1.造模后,大鼠表現(xiàn)為焦躁,倦怠,扎堆角落,皮毛黯淡,糞便時干時稀;大鼠的穿格數(shù)、起立數(shù)、修飾數(shù)與造模前比較均減少(P0.01,或P0.05);2.與空白組比較,模型大鼠胃排空率明顯下降(P0.01);與模型組比較,各治療組大鼠胃排空率較模型組均顯著上升(P0.01),但各治療組組間比較差異無統(tǒng)計學(xué)意義(P0.05);3.與空白組比較,模型大鼠下丘腦中5-HT、DA、NE含量均顯著下降(P0.01);與模型組比較,艾炷灸組大鼠下丘腦中5-HT、DA、NE含量均有所上升,但差異無統(tǒng)計學(xué)意義(P0.05);隔藥餅灸組、逍遙散組大鼠下丘腦中5-HT、DA、NE含量顯著上升(P0.01),但兩組組間比較差異無統(tǒng)計學(xué)意意義(P0.05)。結(jié)論:隔藥餅灸能顯著改善FD肝郁脾虛模型大鼠的胃腸動力,提高其胃排空率,同時顯著增加FD肝郁脾虛模型大鼠下丘腦中5-HT、DA、NE的含量,從而糾正HPA軸的功能亢進(jìn),這可能是其能夠有效治療FD的重要作用機制。
[Abstract]:Objective: to observe the effects of herbal cake moxibustion on gastric emptying rate and hypothalamic serotonin (5-HT) and dopamine (DA), noradrenaline (DA),) (NE) in rats with functional dyspepsia (FD) with liver depression and spleen deficiency. To explore the possible mechanism of moxibustion on FD. Methods: fifty SD rats were randomly divided into 5 groups (10 rats in each group): blank group, model group, moxibustion group and Xiaoyao powder group. The control group was fed with normal diet, and the other 4 groups were made by the method of compound etiology (chronic restraint stress, excessive fatigue diet and tail shaking) for 21 days. Then the patients were treated with moxibustion, moxa cone moxibustion and Xiaoyao powder for 14 days. The moxibustion points of moxibustion group and moxa cone moxibustion group were divided into two groups: group I ("Ganshu", "Peshu", "Weishu") and group II ("Zhangmen", "phengmen", "Zhongwan"). The acupoints of the two groups alternated every other day and each acupoint was continuously moxibustion about 4-5 (about 30min). Xiaoyao Powder group was given Xiaoyao Powder decoction by 1ml/100g. After the treatment, the gastric emptying rate of the rats was calculated by observing the emptying of nutritious semisolid paste. The contents of 5-HTDA and NE in the hypothalamus of rats were detected by HPLC and fluorescence method. Results: 1. After modeling, the rats showed restlessness, burnout, bundled corner, dark fur, dilute dry time of feces, the number of piercing, standing and modification of rats were decreased compared with those before modeling (P0.01, or P0.05); 2. Compared with the blank group, the gastric emptying rate of the model rats decreased significantly (P0.01). Compared with the model group, the gastric emptying rate in each treatment group was significantly higher than that in the model group (P0.01), but there was no significant difference between the treatment groups (P0.05); Compared with the blank group, the contents of 5-HTTDA NE in the hypothalamus of the model rats were significantly decreased (P0.01). Compared with the model group, the hypothalamus of the moxa cone moxibustion group increased the content of 5-HTTDA NE, but the difference was not statistically significant (P0.05). The content of 5-HTTDA NE in hypothalamus of rats in moxibustion group and Xiaoyao powder group increased significantly (P0.01), but there was no significant difference between the two groups (P0.05). Conclusion: moxibustion with herbal cake can significantly improve the gastrointestinal motility and gastric emptying rate of FD rats with liver stagnation and spleen deficiency, and increase the content of 5-HTDDA NE in hypothalamus of rats with FD liver stagnation and spleen deficiency, so as to correct the hyperfunction of HPA axis. This may be an important mechanism for the effective treatment of FD.
【學(xué)位授予單位】：湖南中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R245;R-332
本文編號：2417662
[Abstract]:Objective: to observe the effects of herbal cake moxibustion on gastric emptying rate and hypothalamic serotonin (5-HT) and dopamine (DA), noradrenaline (DA),) (NE) in rats with functional dyspepsia (FD) with liver depression and spleen deficiency. To explore the possible mechanism of moxibustion on FD. Methods: fifty SD rats were randomly divided into 5 groups (10 rats in each group): blank group, model group, moxibustion group and Xiaoyao powder group. The control group was fed with normal diet, and the other 4 groups were made by the method of compound etiology (chronic restraint stress, excessive fatigue diet and tail shaking) for 21 days. Then the patients were treated with moxibustion, moxa cone moxibustion and Xiaoyao powder for 14 days. The moxibustion points of moxibustion group and moxa cone moxibustion group were divided into two groups: group I ("Ganshu", "Peshu", "Weishu") and group II ("Zhangmen", "phengmen", "Zhongwan"). The acupoints of the two groups alternated every other day and each acupoint was continuously moxibustion about 4-5 (about 30min). Xiaoyao Powder group was given Xiaoyao Powder decoction by 1ml/100g. After the treatment, the gastric emptying rate of the rats was calculated by observing the emptying of nutritious semisolid paste. The contents of 5-HTDA and NE in the hypothalamus of rats were detected by HPLC and fluorescence method. Results: 1. After modeling, the rats showed restlessness, burnout, bundled corner, dark fur, dilute dry time of feces, the number of piercing, standing and modification of rats were decreased compared with those before modeling (P0.01, or P0.05); 2. Compared with the blank group, the gastric emptying rate of the model rats decreased significantly (P0.01). Compared with the model group, the gastric emptying rate in each treatment group was significantly higher than that in the model group (P0.01), but there was no significant difference between the treatment groups (P0.05); Compared with the blank group, the contents of 5-HTTDA NE in the hypothalamus of the model rats were significantly decreased (P0.01). Compared with the model group, the hypothalamus of the moxa cone moxibustion group increased the content of 5-HTTDA NE, but the difference was not statistically significant (P0.05). The content of 5-HTTDA NE in hypothalamus of rats in moxibustion group and Xiaoyao powder group increased significantly (P0.01), but there was no significant difference between the two groups (P0.05). Conclusion: moxibustion with herbal cake can significantly improve the gastrointestinal motility and gastric emptying rate of FD rats with liver stagnation and spleen deficiency, and increase the content of 5-HTDDA NE in hypothalamus of rats with FD liver stagnation and spleen deficiency, so as to correct the hyperfunction of HPA axis. This may be an important mechanism for the effective treatment of FD.
【學(xué)位授予單位】：湖南中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R245;R-332
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