【摘要】：目的:觀察電針抗高脂飲食誘導肥胖小鼠的作用及對脂肪組織炎癥相關因子表達的影響。方法:選用40只剛斷乳的SPF級C57BL/6J雄性小鼠,隨機挑選8只普通飼料喂養(yǎng)為正常組,另一組32只高脂飼料喂養(yǎng),將造模成功的16只隨機分為模型組和電針組。比較各組小鼠體重、體脂、血脂等相關指標的差異,用HE染色觀察比較各組小鼠脂肪的形態(tài)學差異,用實時熒光定量PCR檢測各組小鼠脂肪組織中IL-6、TNF-α與MCP-1表達變化。結(jié)果:與模型組相比,電針組小鼠體重、內(nèi)臟脂肪量下降明顯;膽固醇、低密度脂蛋白含量均顯著下降;脂肪組織IL-6、TNF-α、MCP-1的mRNA表達水平亦顯著下降。結(jié)論:電針可以降低肥胖小鼠的體重、體脂,血脂,抑制肥胖小鼠脂肪組織炎癥因子IL-6、TNF-α、MCP-1 mRNA的表達,改善肥胖小鼠炎癥反應狀態(tài),從而實現(xiàn)減肥療效。
[Abstract]:Aim: to observe the effect of electroacupuncture on obesity induced by high fat diet and the expression of inflammatory factors in adipose tissue. Methods: 40 newly weaned SPF grade C57BL/6J male mice were randomly selected as normal diet group and 32 high fat diet groups. The 16 mice were randomly divided into model group and electroacupuncture group. The relative indexes such as body weight, body fat and blood lipid in each group were compared. The morphologic difference of fat in each group was observed by HE staining, and the IL-6, in adipose tissue of each group was detected by real-time fluorescence quantitative PCR. The expression of TNF- 偽 and MCP-1 was changed. Results: compared with the model group, the body weight, visceral fat content, cholesterol and low density lipoprotein contents in the electroacupuncture group were significantly decreased, and the mRNA expression levels of IL-6,TNF- 偽 and MCP-1 in adipose tissue were also significantly decreased. Conclusion: electroacupuncture can reduce the body weight, body fat, blood lipid, inhibit the expression of IL-6,TNF- 偽 and MCP-1 mRNA in adipose tissue of obese mice, improve the inflammatory reaction state of obese mice, and achieve the effect of weight loss.
【作者單位】： 南京中醫(yī)藥大學第二臨床醫(yī)學院;
【基金】：國家自然科學基金(81202742,81403114,81403481,81373747)
【分類號】：R245

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