【摘要】：維藥破布木果(Cordia dichotoma Forst.f.)是紫草科植物破布木的成熟果實(shí),系維醫(yī)常用藥材,在我國主要分布于云南、廣西、廣東、臺灣、福建、新疆等地;國外分布于印度、越南、澳大利亞東北部、菲律賓等國。其具有生濕生熱和清除異常粘液質(zhì),臨床多治療感冒、咳嗽、膽液質(zhì)旺盛、大便不暢、高血壓等。目前破布木果的具體用于藥效成分及相關(guān)藥理作用研究較為欠缺,開展破布木果活性成分的綜合研究,可為破布木果的開發(fā)利用奠定基礎(chǔ)。本論文的研究內(nèi)容分為以下4個部分。1響應(yīng)面法優(yōu)化高剪切提取破布木果多酚的研究:基于高剪切分散乳化技術(shù),通過單因素試驗,分別考察了剪切時間、剪切速度、液料比、乙醇濃度對多酚提取的影響,采用響應(yīng)面法優(yōu)化,得到最佳提取工藝為剪切時間3 min,剪切速度19000 r/min,液料比25 m L/g,乙醇濃度40%時,多酚最大得率8.74 mg GAE/g�；趧恿W(xué)理論,建立了高剪切分散乳化不同剪切速度提取破布木果多酚的提取動力學(xué)方程,其提取過程符合二級動力學(xué)方程,其方程式為:(?)。采用DPPH法測定了破布木果多酚對自由基的清除能力,質(zhì)量濃度為1.0 mg/mL時的清除率與質(zhì)量濃度為0.4 mg/m L Vc溶液的清除率相當(dāng),清除率達(dá)到92%。2響應(yīng)面法優(yōu)化高剪切提取破布木果多糖的研究:基于高剪切分散乳化技術(shù),通過單因素試驗,分別考察了剪切時間、剪切速度、液料比對多糖提取的影響,采用響應(yīng)面法優(yōu)化,得到最佳提取工藝為液料比50 m L/g,剪切速度16000 r/min,剪切時間3 min,多糖最大得率為111.43 mg/g。3對破布木果乙醇提取物的化學(xué)成分進(jìn)行研究:乙醇提取物分別經(jīng)過石油醚、氯仿、乙酸乙酯和正丁醇萃取,各部位經(jīng)過反復(fù)硅膠柱層析、葡聚糖凝膠柱層析和重結(jié)晶技術(shù)得到11個化合物,通過質(zhì)譜、核磁等確定了其中4種化合物,分別為:琥珀酸單乙酯、香草醛、S-2-羥基-3-(4-羥基苯)丙酸和對羥基苯甲酸,以上化合物均為首次從該植物中分離得到。4天然產(chǎn)物吲哚及其衍生物的合成:建立了以四氮唑乙酸為配體,銅催化鄰鹵代苯胺與β-酮酯/β-二酮合成2,3-二取代吲哚的方法,共合成20個底物,此方法無需惰性氣體保護(hù),底物適用性廣泛,產(chǎn)物收率在32%-92%之間,該方法可應(yīng)用于含有此類基團(tuán)的天然產(chǎn)物的合成中。
[Abstract]:(Cordia dichotoma Forst.f.) It is the mature fruit of Rhizoma Aryrhidae. It is a common medicinal material of Uygur Medicine. It is mainly distributed in Yunnan, Guangxi, Guangdong, Taiwan, Fujian, Xinjiang and other places in China, and is distributed in India, Vietnam, northeastern Australia, Philippines and other countries abroad. It is characterized by damp heat generation and removal of abnormal mucus, clinical treatment of colds, cough, exuberant bile fluid, poor stool, high blood pressure and so on. At present, the research on the specific pharmacodynamic components and related pharmacological effects of Puccinia mandshurica is relatively deficient, and the comprehensive study on the active components of Puccinia mandshurica can lay a foundation for its development and utilization. The research contents of this paper are as follows: 1 response surface method is used to optimize the extraction of polyphenols from rags by high shear. Based on the technology of high shear dispersion emulsification, the shear time and shear rate are investigated by single factor experiment. The effects of the ratio of liquid to material and the concentration of ethanol on the extraction of polyphenols were optimized by response surface method. The optimum conditions were obtained as follows: shear time 3 min, shear rate 19000 r / min, liquid-material ratio 25 mL / g, ethanol concentration 40 ml / min. Maximum yield of polyphenols 8.74 mg GAE/g. Based on the kinetic theory, the extraction kinetics equations of polyphenols extracted by high shear dispersion emulsification at different shear rates were established. The extraction process was in accordance with the second-order kinetic equation, and the equation was as follows: (?) The scavenging ability of polyphenols on free radicals was determined by DPPH method. The scavenging rate of polyphenols in 0. 0 mg/mL was equal to that of 0.4 mg/m L Vc solution with mass concentration of 0. 0 mg/mL. A study on the extraction of Polysaccharide from Puccinia fruticosa by High Shear dispersion based on 922.2 response Surface method: based on the technology of high shear dispersion emulsification, the effects of shearing time, shear rate and ratio of liquid to material on the extraction of polysaccharides were investigated by single factor experiment. By using response surface method, the optimum extraction process is obtained as follows: the ratio of liquid to material is 50 mL / g, the shear rate is 16000 r / min, and the shear time is 3 min,. The maximum yield of polysaccharides was 111.43 mg/g.3 to study the chemical constituents of the ethanol extracts of Puccinia officinalis. The ethanol extracts were extracted by petroleum ether, chloroform, ethyl acetate and n-butanol, respectively, and repeated silica gel column chromatography was used for each part of the extracts. Eleven compounds were obtained by dextran gel column chromatography and recrystallization. Four of them were identified by mass spectrometry and nuclear magnetic field, which were: monoethyl succinate, vanillin, etc. S-2-hydroxy-3- (4-hydroxyphenyl) propionic acid and p-hydroxybenzoic acid were isolated from the plant for the first time. 20 substrates were synthesized from o-haloaniline and 尾 -ketone ester / 尾 -diketone by copper-catalyzed synthesis of 2o 3-disubstituted indole. This method has no inert gas protection, wide application of the substrate, and the yield of the product is between 32% and 92%. The method can be applied to the synthesis of natural products containing such groups.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R29

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