本文選題：異常黑膽質成熟劑(ASMq) + 抑郁癥動物模型��； 參考：《新疆醫(yī)科大學》2016年碩士論文

【摘要】：目的觀察異常黑膽質成熟劑(ASMq)對抑郁癥大鼠模型“支配器官”(腦、心、肝)病理變化和超微結構的影響,從組織形態(tài)學角度探討ASMq抗抑郁的可能作用機制。方法將雄性抑郁癥模型大鼠60只,機分為5組:異常黑膽質成熟劑高、中、低劑量組(ASMq給藥劑量為6.0、3.0、1.5 g/kg),陽性對照組(氟西汀,3.5g/kg),抑郁癥模型對照組,每組12只,另設正常對照組(12只)。ASMq各劑量組和陽性對照組連續(xù)灌胃給藥21d,1次/天,末次給藥1h后頸椎脫臼處死動物取出支配器官固定,HE染色,采用光學顯微鏡觀察“支配器官”病理變化,鉛鈾染色,制作常規(guī)電鏡標本,掃描電鏡觀察支配器官超微結構改變。結果與模型組相比,ASMq高劑量組“支配器官”細胞修復性增強,而損傷性組織病理學改變顯著減輕,ASMq低、中劑量組“支配器官”損傷性組織病理變化無顯著差異。抑郁癥模型組“支配器官”超微結構中除了腦組織神經(jīng)元細胞外,其余組織細胞有不同程度的細胞水腫、基質密度明顯降低,細胞變質等超微結構的損傷,但ASMq干預后,腦神經(jīng)膠質細胞水腫明顯降低、細胞基質密度、細胞器稀疏明顯恢復,神經(jīng)細胞突觸部腦水腫明顯的降低、可見輕微空泡樣變;心肌細胞水腫明顯消退、心肌細胞基質密度恢復明顯,未見肌原纖維局灶性肌絲溶解,而心肌細胞內線粒體水腫明顯消退,未見局部有異常收縮帶;肝細胞水腫明顯消退,基質密度明顯恢復、細胞內可見輕微空泡、肝細胞內可見脂滴減小、肝細胞間隙增寬恢復明顯、毛細膽管未擴張,肝細胞間未見膠原纖維增生。結論異常黑膽質成熟劑能夠改善抑郁癥大鼠模型動物“支配器官”病理變化和超微結構的改變。
[Abstract]:Objective to observe the effects of abnomal black bile maturation (ASMQ) on the pathological changes and ultrastructure of brain, heart and liver in a rat model of depression, and to explore the possible mechanism of antidepressant effect of ASMq in terms of histomorphology. Methods Sixty male depression model rats were divided into 5 groups. The dosage of ASMq was 6.0 ~ 3.0 ~ 1.5 g 路kg ~ (-1) 路kg ~ (-1) in the medium and low dose groups, 12 rats in the depression model control group were treated with fluoxetine 3.5 g / kg 路kg ~ (-1), depression model control group (n = 12). In addition, 12 rats in the normal control group and the positive control group were given intragastric administration of the drug for 21 days. The animals were killed for 1 hour after the last administration of the drug. The animals were killed to remove the innervation organ for HE staining, and the pathological changes of the "innervation organ" were observed by optical microscope. The ultrastructural changes of dominant organs were observed by scanning electron microscope (SEM). Results compared with the model group, the repair ability of the "innervation organ" cells in the high dose group of ASMq was enhanced, while the pathological changes of the injured tissue were significantly reduced, but there was no significant difference in the pathological changes of the damaged tissue of the "innervated organ" in the middle dose group. In the model group of depression, the ultrastructure of "innervation organ" was damaged by cell edema, matrix density, cell degeneration and other ultrastructural damage except neurons in brain tissue, but after ASMq intervention. The edema of glial cells, the density of cell matrix, the thinning of organelle, the brain edema in synaptic part of nerve cells, the slight vacuolation, the edema of cardiac myocytes, and the edema of cardiac myocytes disappeared obviously. The matrix density of cardiomyocytes recovered obviously, and no focal myofilament lysis was found in myofibrils, while mitochondria edema disappeared obviously and there was no abnormal contractile zone in myocardial cells, the edema of hepatocytes subsided obviously and the matrix density recovered obviously. There were slight vacuoles in the cells, lipid droplets decreased in the hepatocytes, the gap widened obviously, the capillary bile ducts did not dilate, and the collagen fibers were not proliferated among the hepatocytes. Conclusion abnormal melanoglycoid maturation agent can improve the pathological changes and ultrastructural changes of "innervation organ" in rats with depression.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R29

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