本文選題：放射性肺損傷 + 腫節(jié)風�。� 參考：《廣西醫(yī)科大學》2016年碩士論文

【摘要】：目的：觀察腫節(jié)風和馬藺子素對SD大鼠放射性肺損傷的防護作用。方法：采用隨機數(shù)字表法將120只SD大鼠完全隨機分為正常組(A組)、單純照射組(B組)、腫節(jié)風照射組(C組)、馬藺子素照射組(D組),每組30只。正常組(A組)、單純照射組(B組)灌服等體積的生理鹽水；腫節(jié)風照射組(C組)灌服腫節(jié)風溶液(5g·kg-1·d-1),馬藺子素照射組(D組)灌服馬藺子素的溶液(31mg·kg-1·d-1),照射前連續(xù)灌服1周, 照射后繼續(xù)灌服至肺取出。單純照射組(B組)、腫節(jié)風照射組(C組)、馬藺子素照射組(D組)均采用60Co Y治療機單次20Gy右全肺照射,建立大鼠放射性肺損傷模型,正常組(A組)不予照射。照射后第1、4、8、12、24周取大鼠肺組織蘇木素伊紅染色(HE染色)、馬松三色染色(Masson染色)進行組織病理學觀察；紫外分光光度計檢測肺組織中羥脯氨酸含量；酶聯(lián)免疫吸附測定法(ELISA)檢測血清TNF-α、IL-1β和TGF-β1的濃度；免疫組化法檢測肺組織中腫瘤壞死因子α(TNF-α)和轉(zhuǎn)化生長因子β1(TGF-β1)的表達。結(jié)果：正常組(A組)在各個時間點肺泡結(jié)構(gòu)完整,無炎性細胞滲出和水腫。單純照射組(B組)在照射后1、4、8周出現(xiàn)淋巴細胞及中性粒細胞、巨噬細胞等炎細胞浸潤灶,肺泡毛細血管普遍擴張、肺間質(zhì)充血、水腫,肺泡壁、肺泡間隔逐漸增厚；12、24周,肺泡壁重度增厚,肺泡腔明顯變小甚至缺失,支氣管、血管、肺泡間隔有大量成纖維細胞增生形成纖維化。C組和D組在1、4、8、12周相同時間點也出現(xiàn)上述炎性細胞浸潤灶等病理變化,但較同時期B組輕；24周,肺泡壁重度增厚,D組較B組纖維化程度輕,但C組與B組相比差別不大。血清TNF-α表達水平：在照射后1、4、8周,單純照射組(B組)值均明顯高于C組、D組,差異有統(tǒng)計學意義(P0.05)；而12、24周,與D組比較,差異有統(tǒng)計學意義(P0.05),但與C組比較,差異均無統(tǒng)計學意義(P0.05)。IL-1β表達水平：照射后1、4、8周,單純照射組(B組)值均明顯高于C組、D組,差異有統(tǒng)計學意義(P0.05)；而12、24周,與D組比較差異有統(tǒng)計學意義(P0.05),但與C組比較差異均無統(tǒng)計學意義(P0.05)。血清TGF-β1、肺組織羥脯氨酸含量表達水平：在照射后4、8周,單純照射組(B組)值均明顯高于C組、D組,差異有統(tǒng)計學意義(P0.05)；而12、24周,與D組比較,差異有統(tǒng)計學意義(P0.05),但與C組比較,差異均無統(tǒng)計學意義(P0.05)。肺組織TNF-a的表達：在照射4、8、12周,單純照射組(B組)的表達均明顯高于C組、D組,差異有統(tǒng)計學意義(P0.05)；而24周,與D組比較,差異有統(tǒng)計學意義(P0.05),但與C組比較,差異均無統(tǒng)計學意義(P0.05)。肺組織TGF-β1的表達：在照射后4、8、12周,單純照射組(B組)的表達均明顯高于C組、D組,差異有統(tǒng)計學意義(P0.05)；而24周,與D組比較,差異有統(tǒng)計學意義(P0.05),但與C組比較,差異均無統(tǒng)計學意義(P0.05)。結(jié)論：腫節(jié)風、馬藺子素均能降低早期放射性肺損傷大鼠血清中的TNF-α、IL-1β、TGF-β1含量及肺組織羥脯氨酸、TNF-α、TGF-β1的表達,對早放射性肺損傷具有防護作用,二者無差別。馬藺子素,能減輕放射性肺炎的同時、也能減輕晚期放射性肺纖維化。腫節(jié)風能減輕放射性肺炎,但對減輕晚期放射性肺纖維化作用不明顯。
[Abstract]:Objective: To observe the protective effect of gal and iris on the radiation lung injury of SD rats. Methods: 120 SD rats were randomly divided into normal group (group A), simple irradiation group (group B), group C (group C), group D (group D), normal group (group A), simple irradiation group (B group). Isomer saline (group C) group (group C) administered orisomal solution (5g. Kg-1. D-1), iris irradiate group (group D) to fill in the solution of iris (31mg. Kg-1. D-1), before irradiation for 1 weeks, and continued to be taken to the lung after irradiation. The 60Co Y treatment machine single 20Gy right whole lung irradiation was used to establish a rat model of radionuclide lung injury. The normal group (group A) was not irradiated. After 1,4,8,12,24, the rat lung tissue was stained with hematoxylin (HE), and Masson staining (Masson staining) was used to observe the tissue disease. The ultraviolet spectrophotometer was used to detect the hydroxyproline in the lung tissue. The concentration of TNF- alpha, IL-1 beta and TGF- beta 1 in serum was detected by enzyme linked immunosorbent assay (ELISA); the expression of tumor necrosis factor alpha (TNF- alpha) and transforming growth factor beta 1 (TGF- beta 1) in lung tissue was detected by immunohistochemistry. Results: the normal group (group A) had complete alveolar structure at every time point, without inflammatory cell exudation and edema. In group B, lymphocytes and neutrophils, macrophages and other inflammatory cells infiltrated, alveolar capillaries expanded generally, pulmonary alveolar hyperemia, edema, alveolar wall, and alveolar septum gradually thickened; 12,24 weeks, the alveolar walls were thickened, alveolar cavity became smaller or even missing, bronchi, blood vessels, and alveolar septum were large The fibroblast proliferation and the formation of fibrotic.C group and D group also appeared in the same time point at the same time of 1,4,8,12. But it was lighter than the same period B group. 24 weeks, the alveolar wall thickened and the D group was lighter than the B group, but the C group was not very different from the B group. The serum TNF- alpha expression level: in 1,4,8 weeks after irradiation, simple The value of the group (group B) was significantly higher than that of the group C, and the difference in the group D was statistically significant (P0.05), while the difference was statistically significant (P0.05) compared with the D group (P0.05), but the difference was not statistically significant (P0.05) in the group of C (P0.05).IL-1 beta: the value of the simple irradiation group (B group) was significantly higher than that in the C group, and the difference was statistically significant. (P0.05), and 12,24 weeks, compared with the D group, there was significant difference (P0.05), but there was no significant difference between the C group and the C group (P0.05). The serum TGF- beta 1, the level of hydroxyproline content in the lung tissue was significantly higher than the C group and D group (P0.05) in 4,8 weeks after irradiation, and the difference was statistically significant (P0.05). The difference was statistically significant (P0.05), but compared with the C group, the difference was not statistically significant (P0.05). The expression of TNF-a in the lung tissue was significantly higher than that of the C group (B group) at 4,8,12 weeks, and the difference was statistically significant (P0.05) in the D group (P0.05), but the difference was statistically significant (P0.05) compared with the D group (P0.05), but compared with the C group, the difference was worse than that of the C group. There was no statistically significant difference (P0.05). Expression of TGF- beta 1 in lung tissue: at 4,8,12 weeks after irradiation, the expression of pure irradiation group (group B) was significantly higher than that in group C and group D (P0.05), but the difference was statistically significant (P0.05) compared with that of D group (P0.05), but the difference was not statistically significant (P0.05) compared with that of the C group (P0.05). Conclusion: swelling wind, The contents of TNF- - alpha, IL-1 beta, TGF- beta 1 and the expression of hydroxyproline, TNF- A and TGF- beta 1 in lung tissue of early radioactive lung injury rats can protect the early radioactive lung injury, and there is no difference between the two. It can reduce radiation pneumonitis, but it has no obvious effect on reducing late radiation pulmonary fibrosis.

【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R273

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