本文選題：CO + HO-1�。� 參考：《廣州中醫(yī)藥大學(xué)》2016年碩士論文

【摘要】：目的：腹部手術(shù)后,常出現(xiàn)術(shù)后胃腸功能障礙,導(dǎo)致一系列的臨床癥狀。在促進(jìn)術(shù)后胃腸功能恢復(fù)的臨床研究中,大量的臨床研究表明針刺能減輕術(shù)后胃腸功能障礙的程度,促進(jìn)胃腸功能恢復(fù),目前在臨床上廣泛應(yīng)用。通過前期研究發(fā)現(xiàn),術(shù)后胃腸功能的恢復(fù)可能與胃腸道ICCs修復(fù)再生存在密切的聯(lián)系。CO氣體信號通路是ICCs修復(fù)再生過程中一條重要的途徑,CO是介導(dǎo)胃腸道平滑肌舒張的抑制性神經(jīng)遞質(zhì),當(dāng)神經(jīng)元受刺激時,HO合成CO并釋放,介導(dǎo)神經(jīng)傳導(dǎo)。本課題通過觀察針刺“靳三針”穴組中“足三針”對結(jié)腸吻合術(shù)后SD大鼠胃腸道CO氣體信號通路的影響,探索針刺“足三針”對術(shù)后胃腸功能障礙SD大鼠胃腸功能恢復(fù)的作用機(jī)制。方法：將SD大鼠隨機(jī)分為3組：正常組、模型組、針刺組,模型組及針刺組再按干預(yù)時間的不同分為3天組、5天組、10天組。模型組：行結(jié)腸吻合術(shù)造模。麻醉后沿腹白線于中下腹部切開腹部,與盲腸下端2cm處剪短,縫合結(jié)腸,依次縫合腹膜、肌層和皮膚,術(shù)后不予針刺。針刺組：造模同模型組,術(shù)后按干預(yù)時間不同予以針刺干預(yù)。小腸推進(jìn)率的測量：SD大鼠取材前,用灌胃器抽取lml墨水對大鼠進(jìn)行灌胃,灌胃30min后頸椎脫臼處死,用組織剪剪開腹部,充分暴露腸道,仔細(xì)剝離大腸、小腸,量取小腸全長及幽門至墨水遠(yuǎn)端的距離。參照公式：小腸推進(jìn)率=(墨水行進(jìn)長度／小腸總長度)×100%,計(jì)算每只大鼠的小腸推進(jìn)率。CO、HO-1、HO-2含量的測定：將SD大鼠脫臼處死后,截取盲腸下端吻合口約lcm長,將腸組織清洗干凈,沿縱軸分為三部分,分別用于檢測H0-1、HO-2、C0和電鏡觀察；按照不同的標(biāo)本處理方法將腸組織做前期處理。使用ELISA法測定HO-1、HO-2活性,生化法測定C0含量；使用電鏡觀察Cajal細(xì)胞的超微結(jié)構(gòu)。結(jié)果：1.小腸推進(jìn)率測定結(jié)果：比較各組大鼠小腸推進(jìn)率,正常組與5天針刺組、10天針刺無統(tǒng)計(jì)學(xué)意義(P0.05),3天模型組與5天模型組無統(tǒng)計(jì)學(xué)差異(P0.05),3天針刺組與10天模型組無統(tǒng)計(jì)學(xué)差異(P0.05),5天模型組與10天模型組有統(tǒng)計(jì)學(xué)差異(P0.05),其余各組相比較均有顯著統(tǒng)計(jì)學(xué)意義(P0.01)。2.HO-1活性測定結(jié)果：與3天正常組比較,3天模型組結(jié)果有顯著統(tǒng)計(jì)學(xué)差異(P0.01),3天針刺組結(jié)果有統(tǒng)計(jì)學(xué)差異(P0.05)；與3天模型組比較,5天正常組、5天模型組、5天針刺組、10天正常組、10天模型組、10天針刺組結(jié)果有顯著統(tǒng)計(jì)學(xué)差異(P0.01),3天針刺組結(jié)果有統(tǒng)計(jì)學(xué)差異(P0.05)；與3天針刺組比較,5天正常組、5天針刺組、10天正常組、10天模型組結(jié)果有統(tǒng)計(jì)學(xué)差異(P0.05)；余結(jié)果無統(tǒng)計(jì)學(xué)意義。3.HO-2活性的測定結(jié)果：與正常組比較,3天模型組、3天針刺組、5天模型組、5天針刺組、10天模型組、10天針刺組結(jié)果有統(tǒng)計(jì)學(xué)意義(P0.05)；與3天模型組比較,3天針刺組結(jié)果有統(tǒng)計(jì)學(xué)意義(P0.05),5天模型組、5天針刺組、10天模型組、10天針刺組結(jié)果有統(tǒng)計(jì)學(xué)意義(P0.01)；與3天針刺組比較,5天模型組、5天針刺組、10天模型組、10天針刺組有統(tǒng)計(jì)學(xué)意義(P0.05)；余無統(tǒng)計(jì)學(xué)意義。4.CO含量的測定結(jié)果：與正常組比較,3天模型組、3天針刺組、5天模型組結(jié)果有統(tǒng)計(jì)學(xué)差異(P0.01)；與3天模型組比較,3天針刺組、5天模型組5天針刺組、10天模型組、10天針刺組結(jié)果有統(tǒng)計(jì)學(xué)差異(P0.01)；與3天針刺組比較,5天針刺組、10天模型組、10天針刺組結(jié)果有統(tǒng)計(jì)學(xué)差異(P0.01)；與5天模型組比較,5天針刺組、10天模型組結(jié)果有統(tǒng)計(jì)學(xué)差異(P0.01)。余結(jié)果無統(tǒng)計(jì)學(xué)差異。5.電鏡Cajal細(xì)胞結(jié)果：(1).正常組：Cajal細(xì)胞多成梭形,細(xì)胞核清晰,呈卵圓形,細(xì)胞內(nèi)染色質(zhì)均勻、清晰,細(xì)胞核周圍胞質(zhì)較少,胞內(nèi)細(xì)胞器如：線粒體、內(nèi)質(zhì)網(wǎng)、高爾基體等發(fā)育良好；胞漿內(nèi)中間絲清楚,數(shù)量較多,多分布在細(xì)胞核周圍；基底膜完整；神經(jīng)突觸連接清楚。(2).模型組：①模型3天組：Cajal細(xì)胞凸起明顯減少,胞漿少,胞漿內(nèi)出現(xiàn)數(shù)量較多的空泡；細(xì)胞器數(shù)量明顯減少,細(xì)胞器存在廣泛病變,線粒體腫脹明顯,線粒體嵴斷裂；內(nèi)質(zhì)網(wǎng)擴(kuò)張明顯,粗面內(nèi)質(zhì)網(wǎng)脫顆粒；基底膜不完整。②模型5天組：Cajal細(xì)胞形狀不規(guī)則,細(xì)胞核固縮,異染色質(zhì)趨邊,細(xì)胞內(nèi)胞漿稍豐富,細(xì)胞結(jié)構(gòu)稍清晰；線粒體腫脹,結(jié)構(gòu)不清；中間絲數(shù)量稍多,基底膜不完整。③模型10天組：細(xì)胞內(nèi)胞漿豐富,細(xì)胞器數(shù)量較多,部分結(jié)構(gòu)不清晰；線粒體部分腫脹,可見少量溶酶體；部分粗面內(nèi)質(zhì)網(wǎng)脫顆粒,中間絲清晰,基底膜稍完整。(3)針刺組：①針刺3天組：細(xì)胞內(nèi)胞漿豐富,細(xì)胞器數(shù)量較多,結(jié)構(gòu)較清晰；線粒體部分腫脹；內(nèi)質(zhì)網(wǎng)擴(kuò)張不明顯；中間絲清晰,基底膜欠完整。②針刺5天組：Cajal細(xì)胞形狀規(guī)則,多為梭形,細(xì)胞內(nèi)染色質(zhì)較均勻,細(xì)胞核周圍胞質(zhì)豐富,細(xì)胞器數(shù)量較多,結(jié)構(gòu)清楚,線粒體數(shù)量較多,結(jié)構(gòu)清晰；內(nèi)質(zhì)網(wǎng)無擴(kuò)張；可見少量高爾基體、溶酶體；基底膜較完整。③針刺10天組：Cajal細(xì)胞多成梭形,細(xì)胞核清晰,呈卵圓形,細(xì)胞內(nèi)染色質(zhì)均勻、清晰,細(xì)胞核周圍胞質(zhì)較少,胞內(nèi)細(xì)胞器發(fā)育良好；胞漿內(nèi)中間絲清楚,數(shù)量較多,多分布在細(xì)胞核周圍；基底膜完整；神經(jīng)突觸連接清楚。結(jié)論：1.針刺能改善胃腸功能障礙SD大鼠的小腸推進(jìn)率,針刺組小腸蠕動恢復(fù)時間明顯比模型組恢復(fù)時間縮短。2.針刺能降低術(shù)后應(yīng)激增高的胃腸道HO-1、HO-2活性,使CO合成量減少,CO介導(dǎo)的胃腸道平滑肌舒張功能減弱,促進(jìn)胃腸道蠕動功能的恢復(fù)。3.針刺對手術(shù)損傷帶來的HO-2活性下降具有調(diào)節(jié)作用,能促進(jìn)其回復(fù)正常。4.在POI中,高濃度CO與KIT表達(dá)下降并存,提示CO在高濃度下反而不利于ICC生存
[Abstract]:Objective : To study the effect of acupuncture on gastrointestinal function recovery in SD rats after abdominal surgery . It was used to detect HO - 1 , HO - 2 , C0 and electron microscope respectively .
The intestinal tissues were treated in advance according to different methods of specimen treatment . The contents of HO - 1 and HO - 2 were determined by ELISA .
Results : 1 . There was no significant difference between the 3 - day model group and the 10 - day model group ( P0.05 ) . There was no statistical difference between the three - day model group and the 10 - day model group ( P0.05 ) .
Compared with the 3 - day model group , 5 - day normal group , 5 - day model group , 5 - day acupuncture group , 10 - day normal group , 10 - day model group and 10 - day acupuncture group had significant statistical difference ( P0.01 ) .
Compared with the 3 - day acupuncture group , 5 - day normal group , 5 - day acupuncture group , 10 - day normal group and 10 - day model group had statistical difference ( P0.05 ) ;
The results of HO - 2 activity were as follows : compared with normal group , 3 - day model group , 3 - day acupuncture group , 5 - day model group , 5 - day acupuncture group , 10 - day model group and 10 - day acupuncture group had statistical significance ( P0.05 ) ;
Compared with the 3 - day model group , the results of the three - day acupuncture group had statistical significance ( P0.05 ) , 5 - day model group , 5 - day acupuncture group , 10 - day model group and 10 - day acupuncture group .
Compared with 3 - day acupuncture group , 5 - day model group , 5 - day acupuncture group , 10 - day model group and 10 - day acupuncture group had statistical significance ( P0.05 ) ;
No significant difference was found between the 3 - day model group , the 3 - day acupuncture group and the 5 - day model group ( P0.01 ) .
Compared with the 3 - day model group , the acupuncture group , 5 - day model group , 5 - day acupuncture group , 10 - day model group and 10 - day acupuncture group had statistical difference ( P0.01 ) .
Compared with the 3 - day acupuncture group , the acupuncture group , 10 - day model group and 10 - day acupuncture group had statistical difference ( P0.01 ) .
5 . The results of the 5 - day acupuncture group and the 10 - day model group were significantly different ( P0.01 ) .
The middle filament in the cytoplasm is clear , the quantity is more , the distribution is around the nucleus ;
the basement membrane is complete ;
Neurosynaptic connections are clear . ( 2 ) . The model group : 鈶，

本文編號：1808869