本文選題：腦出血 + 百會透曲鬢��； 參考：《黑龍江中醫(yī)藥大學(xué)》2017年碩士論文

【摘要】：目的:通過針刺"百會"透"曲鬢",觀察在針刺治療過程中凋亡誘導(dǎo)因子AIF蛋白表達(dá)的變化,從而探討其對急性期腦出血大鼠腦組織中AIF表達(dá)的影響,為臨床上治療急性期腦出血提供有利的依據(jù)。方法:75只雄性Wistar大鼠,隨機(jī)分為空白組、模型組和針刺組。其中空白組15只大鼠、模型組和針刺組各30只,每組又平均分為6h,12h,24h,3d,7d五個時間點。模型組和針刺組采用自體血注入法建立腦出血模型。大鼠蘇醒后觀察并記錄其神經(jīng)功能缺損程度,挑選符合條件的模型,對針刺組進(jìn)行針刺"百會"透"曲鬢"的治療。隨后對各組大鼠進(jìn)行系統(tǒng)的實驗觀察:(1)觀察各組大鼠神經(jīng)功能缺損體征及評分情況;(2)采用TUNEL法檢測各組大鼠腦出血后血腫周圍細(xì)胞凋亡情況;(3)采用免疫組化法檢測AIF在腦出血后血腫周圍的動態(tài)表達(dá)。結(jié)果:(1)模型組和針刺組,在6h時開始出現(xiàn)神經(jīng)功能缺損體征;12h-24h,神經(jīng)功能缺損體征持續(xù)加重;在3d時,神經(jīng)功能缺損體征最為嚴(yán)重,7d時神經(jīng)功能缺損體征明顯好轉(zhuǎn)。針刺組神經(jīng)功能評分較相同時間點模型組明顯改善且在12、24h、3d時有顯著差異(P0.01);(2)TUNEL法檢測結(jié)果顯示:與模型組比較,針刺組在各時間點神經(jīng)細(xì)胞凋亡數(shù)量均出現(xiàn)不同程度的減少,且在24h、3d、7d時有顯著差異(P0.01);(3)免疫組化檢測結(jié)果顯示:與模型組比較,針刺組在各時間點AIF蛋白表達(dá)水平均有不同程度的降低,且在24h、3d、7d時有顯著差異(P0.01)。結(jié)論:(1)針刺"百會"透"曲鬢"可以減輕腦出血大鼠神經(jīng)功能缺損體征;(2)針刺"百會"透"曲鬢"能夠有效抑制腦出血后神經(jīng)細(xì)胞凋亡的產(chǎn)生;(3)針刺"百會"透"曲鬢"可以減少凋亡誘導(dǎo)因子AIF的表達(dá),通過抑制AIF的表達(dá),對腦出血后血腫周圍腦組織起到保護(hù)作用。
[Abstract]:Objective: to observe the expression of apoptosis-inducible factor (AIF) protein in the brain of rats with acute cerebral hemorrhage (ICH), and to investigate the effect of Acupuncture on the expression of Apoptosis-inducible Factor (AIF) in the brain of rats with acute intracerebral hemorrhage (ICH). To provide a favorable basis for clinical treatment of acute cerebral hemorrhage. Methods 75 male Wistar rats were randomly divided into blank group, model group and acupuncture group. There were 15 rats in the blank group, 30 rats in the model group and 30 in the acupuncture group. The model group and acupuncture group were used to establish the model of intracerebral hemorrhage by autologous blood injection. After waking up, the rats were observed and recorded the degree of nerve function defect, and the models were selected, and the acupuncture group was treated with acupuncture "Baihui" and "Baihui". Then the rats in each group were observed systematically. (1) the signs and scores of nerve function defect in each group were observed. (2) TUNEL method was used to detect apoptosis around hematoma in rats after intracerebral hemorrhage) Immunohistochemical method was used to detect the apoptosis of the cells around the hematoma. The dynamic expression of AIF around hematoma after intracerebral hemorrhage was measured. Results in the model group and the acupuncture group, the signs of nerve function defect began to appear at 6 h after 12 h 24 h, and the signs of nerve function defect continued to increase, and at 3 d, the signs of nerve function defect were obviously improved at 7 d after the most serious signs of nerve function defect. The score of nerve function in acupuncture group was significantly improved than that in model group at the same time point, and there was significant difference at 12 ~ 24 hours after 3 days. The results of Tunel method showed that the number of neuronal apoptosis in acupuncture group was decreased in varying degrees compared with model group. The results of immunohistochemical analysis showed that the expression of AIF protein in acupuncture group was significantly lower than that in model group at each time point, and there was a significant difference in P0.01protein expression at 24 h / 3 d ~ (-1) and 3 d / 7 ~ (th) after acupuncture compared with the model group, the expression of AIF protein in the acupuncture group was significantly lower than that in the model group at 7 days after the acupuncture treatment, and there was a significant difference between the two groups at 24 h / 3 d ~ (-1). Conclusion the acupuncture of "Baihui" ("Baihui") can reduce the signs of nerve function defect in rats with intracerebral hemorrhage. Acupuncture "Baihui" "diaphanous" can effectively inhibit the production of neuronal apoptosis after intracerebral hemorrhage and acupuncture "Baihui" can effectively inhibit the occurrence of neuronal apoptosis after intracerebral hemorrhage and acupuncture "Baihui" ("Baihui") can effectively inhibit the occurrence of neuronal apoptosis after intracerebral hemorrhage. The expression of apoptosis-inducing factor AIF could be reduced by "qu Si". By inhibiting the expression of AIF, it can protect the brain tissue around hematoma after intracerebral hemorrhage.
【學(xué)位授予單位】：黑龍江中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R245

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