本文選題：砂地柏 + 總黃酮��； 參考：《新疆醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:研究維吾爾藥砂地柏總黃酮(JSTF)的抗炎鎮(zhèn)痛及免疫調(diào)節(jié)作用,為其深入開發(fā)提供基礎(chǔ)數(shù)據(jù)。方法:(1)采用二甲苯致小鼠耳腫脹、小鼠毛細(xì)血管通透性、大鼠棉球肉芽腫以及蛋清、組胺和角叉菜膠致大鼠足趾腫脹實(shí)驗(yàn),以阿司匹林為陽性對照,觀察JSTF對炎癥的抑制作用;(2)采用冰醋酸致小鼠扭體法、熱板法和甲醛致小鼠疼痛實(shí)驗(yàn),觀察JSTF的鎮(zhèn)痛作用;(3)分別采用碳粒廓清和血清溶血素測定實(shí)驗(yàn)觀察JSTF對小鼠非特異性和特異性體液免疫功能的影響。結(jié)果:(1)低、中、高劑量組的JFST(125、250、500 mg/kg)能明顯抑制二甲苯致小鼠耳廓腫脹的發(fā)生(P0.05),其抑制率分別為(16.22%,40.67%,51.78%)。低、中、高劑量組的JFST(125、250、500 mg/kg)也能明顯抑制小鼠毛細(xì)血管通透性的發(fā)生(P0.05),其抑制率分別為(11.63%,32.56%,53.49%)。在角叉菜膠引起大鼠足趾腫脹實(shí)驗(yàn)中,除了第一個(gè)時(shí)間段內(nèi)JSTF低劑量組和模型組比較無統(tǒng)計(jì)學(xué)意義以外,其他各組、各時(shí)間段內(nèi)均抑制大鼠足趾腫脹。對棉球引起的大鼠肉芽腫實(shí)驗(yàn)中,JSTF低、中、高劑量組(125、250、500 mg/kg)與阿司匹林組(200 mg/kg)比較有明顯的抑制作用,與模型組相比較有統(tǒng)計(jì)學(xué)差異(P0.05),其抑制率分別低劑量組為15.38%、中劑量組為18.48%、高劑量組為22.02%,阿司匹林組為43.11%。在蛋清和組胺引起大鼠足趾腫脹試驗(yàn)中,除了JSTF低劑量組(125mg/kg)在各個(gè)時(shí)間段內(nèi)未能抑制大鼠足趾腫脹以外,其它給藥組均明顯的抑制了大鼠足趾腫脹,與模型組比較,有統(tǒng)計(jì)學(xué)意義(P0.05,P0.01)。(2)JSTF各劑量組能顯著減少醋酸引起的小鼠扭體反應(yīng)次數(shù)(P0.01)和延長小鼠熱板痛閾值(P0.01,P0.05)。JSTF明顯抑制第一和第二時(shí)相反應(yīng)(P0.01,P0.05),而陽性對照組僅在第二時(shí)相出現(xiàn)抑制反應(yīng)。(3)JFST可以提高免疫低下小鼠碳粒廓清指數(shù)K,尤其是500mg/kg劑量可使碳粒廓清指數(shù)顯著升高(P0.01);與模型組比較JFST各劑量組也可以提高免疫低下小鼠血清溶血素生成(P0.05)。結(jié)論:JSTF具有明顯的抗炎鎮(zhèn)痛及免疫調(diào)節(jié)作用,其作用機(jī)制需要進(jìn)一步研究。
[Abstract]:Aim: to study the anti-inflammatory, analgesic and immunomodulatory effects of JSTF in order to provide basic data for its further development. Methods the mice ear swelling induced by xylene, capillary permeability, cotton ball granuloma and egg white, histamine and carrageenin induced toe swelling in rats were used. Aspirin was used as the positive control. To observe the inhibitory effect of JSTF on inflammation in mice by acetic acid-induced writhing test, hot plate method and formaldehyde induced pain test. The analgesic effect of JSTF was observed. The effects of JSTF on nonspecific and specific humoral immune function in mice were observed by carbon particle clearance and serum hemolysin assay. Results JFST(125250500 mg / kg in low, middle and high dose groups could significantly inhibit the development of auricle swelling induced by xylene in mice (P 0.05). The inhibition rates were 40.67 and 51.78, respectively. JFST(125250500 mg / kg in low, middle and high dose groups also significantly inhibited the development of capillary permeability in mice (P 0.05), and the inhibition rates were 11.63% and 32.56%, respectively. In the experiment of carrageenin induced toe swelling in rats, except for the low dose group of JSTF and the model group in the first time period, there was no significant difference between the two groups, but in other groups, the swelling of the toes of rats was inhibited in each time period. In the experiment of cotton ball induced granuloma in rats, JSTF was significantly inhibited in the low, medium and high dose groups (125,250,500 mg / kg) and aspirin group (200mg / kg). Compared with the model group, the inhibition rate was 15.38 in the low dose group, 18.48 in the middle dose group, 22.02 in the high dose group and 43.11 in the aspirin group. In the test of toe swelling induced by egg white and histamine in rats, except for the low dose group of JSTF (125 mg / kg), which could not inhibit the swelling of rat toe in every time period, the other groups obviously inhibited the swelling of the toes of rats, compared with the model group. There was statistical significance that P0.05P0.01P0.01P0.05JSTF could significantly reduce the times of writhing reaction induced by acetic acid in mice and prolong the pain threshold of hot plate in mice P0.01P0.05N. JSTF significantly inhibited the first and 02:00 phase responses, while the positive control group only showed inhibition at 02:00. JFST could increase the clearance index of carbon particles in immunocompromised mice, especially the dosage of 500mg/kg could significantly increase the clearance index of P0.01G, and the serum hemolysin production of hypoimmunized mice was also increased by different doses of JFST compared with the model group. ConclusionJSTF has obvious anti-inflammatory, analgesic and immunomodulatory effects, and its mechanism needs further study.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R29

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