本文選題：原發(fā)性肝癌 + 藏藥��； 參考：《華中師范大學(xué)學(xué)報(bào)(自然科學(xué)版)》2017年03期

【摘要】：本實(shí)驗(yàn)采用快速溶劑萃取法制備收集100種常用藏藥的環(huán)己烷、乙酸乙酯和甲醇提取物,并運(yùn)用MTT法對(duì)兩個(gè)肝癌細(xì)胞株HepG2和SMMC-7721進(jìn)行各提取物的抗肝癌活性測(cè)試,同時(shí)選用L-02細(xì)胞來評(píng)價(jià)其體外毒性.抗肝癌活性結(jié)果顯示100種常用藏藥中17種藏藥材有良好的抗肝癌活性,其IC_(50)值均小于150μg/mL,其中馬兜鈴、藍(lán)翠雀花、甘青青蘭、掌葉橐吾的乙酸乙酯提取物顯示出顯著的抗肝癌活性,其IC_(50)值均小于50μg/mL,但馬兜鈴乙酸乙酯提取物對(duì)正常肝細(xì)胞L-02顯示出一定的毒性.在進(jìn)一步的抗HepG2肝癌活性驗(yàn)證中,結(jié)果顯示藍(lán)翠雀花乙酸乙酯提取物和甘青青蘭乙酸乙酯提取物具有顯著的抗肝癌活性,兩者在抗HepG2肝癌細(xì)胞株活性中皆顯示出顯著的時(shí)效和量效關(guān)系.運(yùn)用倒置顯微鏡以及Hoechst 33258熒光染色法對(duì)經(jīng)過藍(lán)翠雀花乙酸乙酯提取物或甘青青蘭乙酸乙酯提取物作用的HepG2細(xì)胞形態(tài)進(jìn)行觀察,結(jié)果提示藍(lán)翠雀花和甘青青蘭乙酸乙酯提取物可能是通過誘導(dǎo)HepG2細(xì)胞凋亡而顯示抗癌活性.
[Abstract]:The extraction of cyclohexane, ethyl acetate and methanol from 100 common Tibetan medicines was prepared by rapid solvent extraction. The antitumor activity of the extracts from two hepatoma cell lines, HepG2 and SMMC-7721, was tested by MTT method.At the same time, L-02 cells were selected to evaluate its toxicity in vitro.The results of anti-hepatocarcinoma activity showed that 17 of the 100 common Tibetan medicines had good anti-liver cancer activity, and their ICSP 50) values were less than 150 渭 g / mL, including Aristolochia spp.The ethyl acetate extract of Ligularia palliaria showed significant anti-hepatoma activity, and its ICL 50 value was less than 50 渭 g / mL, but the ethyl acetate extract from Aristolochia spp showed a certain toxicity to L-02 of normal hepatocytes.The results showed that the ethyl acetate extract and the ethyl acetate extract from blue blue blue had significant anti-hepatoma activity in the further test of anti-hepatoma activity of HepG2.Both of them showed a significant time-dependent and dose-response relationship in anti HepG2 hepatoma cell line activity.The morphology of HepG2 cells treated with ethyl acetate extract from blue sparrow flower or ethyl acetate extract from blue blue blue was observed by inverted microscope and Hoechst 33258 fluorescence staining.The results suggested that the ethyl acetate extracts from the blue sparrow and the blue blue blue may have anticancer activity by inducing apoptosis of HepG2 cells.
【作者單位】： 中南民族大學(xué)藥學(xué)院;華中科技大學(xué)同濟(jì)醫(yī)學(xué)院附屬同濟(jì)醫(yī)院藥學(xué)部;青海民族大學(xué)科學(xué)科技處;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(81573561) 青海省自然科學(xué)基金項(xiàng)目(2012-Z-904)
【分類號(hào)】：R29

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