本文選題：正骨理筋手法　切入點(diǎn)：完全弗氏佐劑　出處：《河南中醫(yī)藥大學(xué)》2016年碩士論文

【摘要】：目的:通過觀察中醫(yī)骨傷科理筋手法對完全弗氏佐劑性炎癥痛大鼠疼痛閾值的影響及測量手法局部治療后腺苷的含量,探討手法緩解炎癥痛的效果及其鎮(zhèn)痛作用機(jī)制,為臨床理筋手法局部鎮(zhèn)痛作用提供理論依據(jù)。方法:選取健康SD雌性大鼠24只,隨機(jī)分為空白組(A1)、弗氏佐劑模型組(A2)、手法治療組(A3),每組均為8只。A1空白組不給予造模處理,亦不給予手法治療,在實驗開始以后,將大鼠用固定器進(jìn)行固定,固定時間與手法治療組相同;A2弗氏佐劑模型組于實驗開始第一天,在大鼠右后足墊部皮下注射完全弗氏佐劑0.1ml致炎,建立大鼠佐劑性炎癥疼痛模型,不給予治療,其它處理同空白組。A3手法治療組于實驗開始第一天,在大鼠右后足墊部皮下注射弗氏完全佐劑0.1ml致炎,建立大鼠佐劑性炎癥疼痛模型,并于造模后第4日對大鼠進(jìn)行手法治療,手法選取理筋手法中常用的中指點(diǎn)壓法。在安靜環(huán)境中,將大鼠固定在特制的動物固定器上,頭部及身體固定,使動物處于清醒狀態(tài)下,選取足部涌泉穴給予點(diǎn)壓法治療,時間20min,治療完畢后測量并記錄大鼠熱痛閾值,然后處死取材。通過測量大鼠的熱敏縮足反射時間觀察痛閾值改變,測量腳掌周徑以評估大鼠足腫脹程度對模型進(jìn)行評價。分別于三個時間點(diǎn)(造模前1天,造模后4天治療前,治療后)應(yīng)用HWS-26電熱恒溫水浴鍋測定大鼠熱疼痛閾值,并制作腳掌組織病理切片,手法干預(yù)后用ELISA試劑盒檢測腺苷的含量。結(jié)果:1.大鼠一般情況:空白組大鼠皮毛光潤,精神狀態(tài)好,喜動,反應(yīng)靈敏,飲食正常;弗氏模型組A2、手法治療組A3造模后,大鼠致炎側(cè)足部出現(xiàn)紅腫,24h左右達(dá)到高峰,皮毛失去光澤,精神萎靡,少活動,致炎側(cè)足部不敢著地,呈跛行,持續(xù)3天后癥狀逐漸減輕。2.造模前各組大鼠之間腳掌周徑比較,差異不明顯(P0.05),造模后,模型大鼠腳掌周徑明顯增加,24h左右達(dá)到高峰,與對側(cè)腳掌周徑比較,有顯著性差異(P0.05),與空白組腳掌周徑比較,有顯著性差異(P0.05)。3.組織病理學(xué)研究顯示:模型大鼠肌肉組織可見炎性細(xì)胞浸潤,肌纖維的萎縮,肌纖維間隙增寬,小血管增生等病理改變等,與正常組比較,有明顯差異。4造模前大鼠各組間熱痛閾值比較,無顯著性差異(P0.05);造模后各組大鼠熱痛閾值,弗氏模型組A2、手法治療組A3與空白組A1比較,明顯降低,均有顯著性差異(P0.05);手法治療組A3治療后熱痛閾值明顯升高,與治療前比較有顯著性差異(P0.05);各組大鼠治療后熱痛閾值比較,空白組A1與其他各組比較均有顯著性差異(P0.05),弗氏模型組A2與手法治療組A3比較均有顯著性差異(P0.05);治療后各組大鼠腺苷濃度,空白組A1與弗氏模型組A2比較無顯著性差異(P0.05),手法治療組A3大鼠外周組織腺苷含量明顯升高,與空白組A1比較有顯著性差異(P0.05),與模型組A2比較有顯著性差異(P0.05)。結(jié)論:1.完全弗氏佐劑作為一種抗原復(fù)合物,可以使大鼠足部注射部位局部產(chǎn)生紅、腫及熱痛敏行為反應(yīng),并引起組織病理學(xué)的改變,所造模型是一種常用的外周炎癥痛模型。2.理筋手法局部鎮(zhèn)痛效果顯著,能夠顯著提高炎性疼痛模型大鼠的熱疼痛閾值。3.理筋手法治療能夠升高炎癥疼痛大鼠局部神經(jīng)遞質(zhì)腺苷濃度,理筋手法局部鎮(zhèn)痛作用可能通過局部腺苷釋放而起作用。
[Abstract]:Objective: To observe the content of adenosine by TCM traumatology according to massage effect on pain threshold in rats with complete Freund's adjuvant induced inflammatory pain and measurements after local treatment practices to alleviate inflammation pain effect and its mechanism of analgesic effect, for clinical manipulation for local analgesia and provide a theoretical basis. Methods: healthy female SD 24 rats were randomly divided into control group (A1), Freund's adjuvant model group (A2), massage group (A3), each group had 8 rats in.A1 group were not given blank model, nor give treatment, at the start of the experiment, the rats were treated with fixation fixation. Fixed time and manipulative treatment group; A2 Freund's adjuvant model group at the beginning of the first day, in the rat right hind foot pad subcutaneous injection of complete Freund's adjuvant 0.1ml induced inflammation, establishment of rat adjuvant inflammatory pain model, not treated, other treatment with the control group.A3 Manipulative treatment group at the beginning of the experiment the first day, the foot pad of the subcutaneous injection of complete Freund's adjuvant induced arthritis in 0.1ml rats right after the establishment of rat adjuvant induced inflammatory pain model, and on the fourth day after the rats were subjected to treatment, technique selection pointing pressure method commonly used in manipulation in. In the quiet environment, the rats were fixed in the special animal fixer, head and body fixed, the animal in the awake state, select the foot Yongquan point to pressure therapy, treatment time 20min, after the measurement and recording of rat heat pain threshold, and then sacrificed. Observe the change through the thermal pain threshold measurement the rat paw withdrawal time, measuring foot circumference to evaluate rat paw swelling to evaluate the model. In three time points (1 days before modeling, after modeling 4 days before treatment, after treatment) were measured by HWS-26 thermal pain electric thermostatic water bath The pain threshold value, and make the sole histological technique after intervention with ELISA kit for detection of adenosine content. Results: 1. the general condition of the rats: control group rat fur was smooth, good mental state, dynamic Hi, sensitive reaction, normal diet; model group A2 Freund, manipulative treatment group A3 after molding rat inflammatory side foot swelling, peaked around 24h, dull coat, listlessness, less activity, inflammatory side foot not landing, a limp, continued for 3 days after the symptoms gradually reduce.2. between the rats in the foot circumference, no significant difference (P0.05), after modeling week, foot rat model of diameter increased, peaked around 24h, and on the side of the foot circumference, there was significant difference (P0.05) compared with the control group, diameter foot week, there was significant difference (P0.05) study showed.3. pathology: inflammatory cells of muscle tissue in a rat model of infiltration. Muscle Fiber atrophy, muscle fiber gap widened, vascular proliferation and other pathological changes, compared with the normal group, there was significant difference between.4 rats and heat pain threshold comparison mode, no significant difference (P0.05); after the model rats heat pain threshold, the Freund model group A2 technique the treatment group with the control group, A3 A1, was significantly reduced, there were significant differences (P0.05) technique; treatment group A3 thermal pain threshold was significantly increased after treatment, there was significant difference compared with before treatment (P0.05); compare the thermal pain threshold of rats after the treatment, the control group of A1 and the other groups were significant the difference (P0.05), model group A2 and Freund's manipulative treatment group A3 had significant difference (P0.05); adenosine concentration in rats after treatment, there was no significant difference between blank group A1 and Freund's A2 in the model group (P0.05), treatment group A3 rat peripheral tissues of adenosine increased significantly. With the control group, A1 There were significant differences between (P0.05), there was significant difference compared with the model group A2 (P0.05). Conclusion: 1. complete Freund's adjuvant is an antigen complex, can make the foot of the injection site of locally produced red, swollen and heat hyperalgesia behavior, and cause the change of histopathology, the the model is a common peripheral inflammatory pain model.2. manipulation of local analgesic effect significantly, can significantly improve the thermal pain threshold of.3. inflammatory pain in a rat model of manipulation therapy can increase local neurotransmitter adenosine concentration in inflammatory pain rats, tendon hand local analgesic effect by local adenosine release play a role.

【學(xué)位授予單位】：河南中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2016
【分類號】：R274

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