本文選題：燥證　切入點(diǎn)：云南春燥環(huán)境　出處：《云南中醫(yī)學(xué)院》2017年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的:通過(guò)觀察云南春燥環(huán)境對(duì)小鼠生物表征和體內(nèi)津液的影響,以探討云南春燥的內(nèi)在本質(zhì),揭示其證候的微觀機(jī)制,并為建立云南春燥實(shí)驗(yàn)動(dòng)物模型提供思路。方法:以中醫(yī)病因?qū)W說(shuō)為指導(dǎo),將實(shí)驗(yàn)小鼠隨機(jī)分為正常對(duì)照組,氣候組,食物組和氣候食物組,利用人工氣候箱及香燥食物模擬云南春燥環(huán)境并作用于各組實(shí)驗(yàn)小鼠,觀察各組小鼠的一般生物表征的變化,采用HE染色法觀察各組小鼠皮膚組織,肺組織,大腸組織的病理形態(tài)學(xué)改變,記錄各組小鼠皮膚組織,肺組織及大腸組織的干濕重計(jì)算其各組織的含水率的變化。結(jié)果:與正常對(duì)照組小鼠相比,氣候組小鼠從施加因素第3天起出現(xiàn)打斗現(xiàn)象,尾部可見(jiàn)傷痕,并逐漸表現(xiàn)出煩躁易怒的狀態(tài),毛發(fā)逐漸稀疏,缺少光澤,爪甲顏色暗紫;食物組小鼠在施加因素后可見(jiàn)糞便便質(zhì)略顯干燥;氣候食物組小鼠在施加因素后皮毛逐漸稀疏,易脫落,缺乏光澤,前期偶有打斗現(xiàn)象,尾部可見(jiàn)傷痕,后期趨于平靜,爪甲顏色正常。與正常對(duì)照組小鼠第7天相比,氣候組小鼠第7天體重降低(P0.05),肺組織出現(xiàn)病理形態(tài)學(xué)改變,肺組織含水率下降(P0.01),大腸組織含水率下降(P0.01);食物組小鼠第7天可見(jiàn)肺組織及大腸組織病理形態(tài)學(xué)改變,肺組織含水率下降(P0.01),大腸組織含水率下降(P0.01);氣候食物組小鼠第7天體重下降(P0.01),肺組織出現(xiàn)病理形態(tài)學(xué)改變,肺組織含水率下降(P0.01),大腸組織含水率下降(P0.01)。與正常對(duì)照組小鼠第14天相比,氣候組小鼠第14天體重降低(P0.01),肺組織出現(xiàn)病理形態(tài)學(xué)改變,肺組織含水率下降(P0.01),大腸組織含水率下降(P0.05),小鼠14天的飲水量增加(P0.01),小便量減少(P0.01);食物組小鼠第14天肺組織含水率下降(P0.05),大腸組織含水率下降(P0.01),小鼠14天的飲水量增加(P0.05),小便量減少(P0.01);氣候食物組小鼠第14天可見(jiàn)肺組織病理形態(tài)學(xué)改變,肺組織含水率下降(P0.01),小鼠14天的飲水量增加(P0.01),小便量減少(P0.05)。結(jié)論:1.云南地區(qū)獨(dú)特的地域環(huán)境可致使小鼠出現(xiàn)干燥的生物表征變化,且隨著施加氣候因素時(shí)間的延長(zhǎng),這種變化越來(lái)越明顯,而隨著施加飲食因素時(shí)間的延長(zhǎng),這種變化逐漸減弱。2.云南春燥環(huán)境中的氣候因素可影響小鼠肺組織病理形態(tài)學(xué)的改變,且能夠減少肺組織和大腸組織的含水率。3.云南春燥環(huán)境中的食物因素在實(shí)驗(yàn)初期可影響小鼠肺組織和大腸組織病理形態(tài)學(xué)的改變,且能夠減少肺組織和大腸組織的含水率。4.云南春燥環(huán)境中的氣候因素和飲食因素均能對(duì)小鼠體內(nèi)津液的整個(gè)生成、輸布和代謝過(guò)程產(chǎn)生影響,且肺與大腸津液下降到一定程度時(shí),會(huì)影響兩者出現(xiàn)病理形態(tài)學(xué)的改變。
[Abstract]:Objective: to explore the intrinsic nature of Yunnan spring dryness and reveal the microscopic mechanism of its syndromes by observing the effect of Yunnan spring dryness environment on biological characterization and body fluid in mice. Methods: the experimental mice were randomly divided into normal control group, climate group, food group and climate food group under the guidance of the theory of etiology of traditional Chinese medicine. The artificial climate box and fragrant dry food were used to simulate Yunnan spring dryness environment and acted on experimental mice in each group. The changes of general biological characteristics of each group were observed, and the skin and lung tissues of each group were observed by HE staining method. The pathomorphological changes of large intestine tissue were recorded, and the changes of moisture content in the skin, lung and large intestine tissue were calculated. Results: compared with the normal control group, the changes of moisture content in each tissue of each group were compared with those of the normal control group. From the third day after the application of the factors, the climate group mice appeared the phenomenon of fighting, the tail visible scars, and gradually showed irritable and irritable state, hair gradually sparse, lack of luster, claw color dark purple; In the food group, the fecal stool was slightly dry after applying the factors, and the skin of the climate food group was gradually sparse, easy to fall off, lacking of gloss, occasional fighting in the early stage, visible scars in the tail, and the later period tended to be calm, after the application of these factors, the mice in the food group showed a slight dry fecal feces. The color of claw armour was normal. Compared with the control group mice on the 7th day, the mice in the climate group lost their body weight on the 7th day, and the lung tissue showed pathomorphologic changes. The decrease of water content in lung tissue (P0.01A) and the decrease of water content in large intestine (P0.01C) were observed in mice of food group on the 7th day, the histopathological changes of lung tissue and large intestine tissue were observed in the food group. The contents of water in lung tissue and large intestine were decreased (P 0.01), and the weight of mice in climate food group was decreased on day 7 (P 0.01), and the pathological morphology of lung tissue was observed. Compared with the control group on the 14th day, compared with the control group, the mice in the climate group lost their body weight on the 14th day, and the pathological morphology of the lung tissue was observed, compared with the control group on the 14th day, compared with the control group on the 14th day, compared with the control group, the mice in the climate group lost their body weight on the 14th day. The water content of lung tissue decreased P0.01A, the water content of large intestine tissue decreased P0.05N, the drinking water of mice increased P0.01D and the amount of urine decreased P0.01G on the 14th day of food group, the water content of lung tissue decreased P0.05N on the 14th day of food group, the water content of large intestine tissue decreased P0.01, and the drinking water of mice was drinking water for 14 days. The increase of P0.05, the decrease of urine volume and the change of pathomorphology of lung tissue were observed on the 14th day in the climate food group. The decrease of water content in lung tissue (P0.01A), the increase of drinking water in mice (14 days) and the decrease of urine volume (P0.05). Conclusion: 1. The special regional environment in Yunnan region can cause dry biological characterization changes in mice, and with the extension of the time of applying climatic factors, This change became more and more obvious, but with the prolongation of the time of applying dietary factors, the change gradually weakened .2.Climatic factors in spring dryness environment in Yunnan could affect the pathological changes of lung tissue in mice. Food factors in spring dryness environment in Yunnan could influence the pathomorphological changes of lung and large intestine tissues in mice at the beginning of the experiment. And it can reduce the moisture content of lung tissue and large intestine tissue. 4. The climatic factors and diet factors in spring dryness environment in Yunnan can affect the whole production, distribution and metabolism of body fluid in mice. When lung and large intestine fluid decreased to a certain extent, the pathomorphologic changes were affected.
【學(xué)位授予單位】：云南中醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R-332;R228

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