本文關鍵詞： 壯藥 大葉金花草 質量評價 生藥鑒定 一測多評 HPLC指紋圖譜　出處：《廣西中醫(yī)藥大學》2016年碩士論文　論文類型：學位論文

【摘要】：目的:對壯藥大葉金花草進行真?zhèn)�、�?yōu)劣評價,初步建立大葉金花草質量評價方法。方法:通過文獻考證明確其歷史沿革;利用原植物鑒定、性狀鑒定與理化鑒定的方法進行生藥學鑒定研究;以顯微鑒定的方法鑒別大葉金花草與小金花草;按《中國藥典》2015年版的方法進行藥材水分、灰分、浸出物等檢查項目相關研究;采用HPLC一測多評法,同時測定其中4種成分的含量;對其進行高效液相指紋圖譜研究。結果:(1)明確大葉金花草的品種來源、功能效用,為大葉金花草的臨床應用提供依據(jù)。(2)原植物形態(tài)、藥材性狀特征與薄層色譜均具有鑒別意義。(3)大葉金花草孢子為一側凹陷的卵球形,根莖具有1個周韌型管狀中柱,中央有髓,葉柄具1個“U”形維管束,葉表皮細胞壁不增厚;小金花草孢子為球形或類三角形的四面體,根莖分體中柱周韌型,葉柄具2個維管束,葉表皮細胞外壁略增厚。(4)大葉金花草藥材根及根莖水分含量在6.64%~10.35%,總灰分含量在8.50%~15.91%,酸不溶性灰分在4.98%~11.10%,浸出物含量在7.95%~21.40%之間;全草藥材含水量為7.45%~12.24%,總灰分為5.04%~11.29%,酸不溶性灰分范圍為1.39%~6.58%,浸出物含量為24.96%~38.05%;地上部分水分含量幅度為8.35%~15.62%,總灰分含量幅度為3.94%~5.00%,酸不溶性灰分幅度為0.17%~0.80%,浸出物含量幅度為26.61%~35.76%。(5)一測多評所得的含量計算結果與外標法所得結果無顯著性差異,在不同的實驗條件下,各成分之間的校正因子重現(xiàn)性好(RSD3%),因此一測多評法可以應用于大葉金花草的質量評價。(6)建立了大葉金花草指紋圖譜共有模式,標定了14個共有峰,確定了其中4個主要色譜峰所對應的物質。結論:以上實驗及研究對壯藥大葉金花草藥材質量進行了初步評價,為其臨床應用的安全、有效和可控提供參考。本論文研究內容經(jīng)科技查新證實,目前尚未見與上述研究相同的國內外文獻報道。
[Abstract]:Objective: to evaluate the true and false quality of Flos aurantia and to establish a method of quality evaluation. Methods: the historical evolution was confirmed by textual research. The methods of original plant identification, character identification and physicochemical identification were used to identify the pharmacognosy. The method of microscopical identification was used to identify Amomum macrophylla from Flos aurantia. According to the method of 2015 edition of Chinese Pharmacopoeia, the related studies on water, ash and extract of medicinal materials were carried out. The content of four components was determined by HPLC method. The results showed that the origin and function of the species were determined, which provided the basis for clinical application. The characteristics of medicinal materials and TLC have the distinguishing significance. 3) the spores are ovoid in one side of the sag, the rhizome has a round and tough tubular column, and the center is myelinated. The petiole has a "U" vascular bundle, and the cell wall of the leaf epidermis is not thickened. The spores are globose or triangular tetrahedron, rhizomatous in the column round tough type, petiole with two vascular bundles. The content of water in the root and rhizome of Phyllostachys davidii was 6.64 and 10.35, and the total ash content was 8.500.91%. The acid insoluble ash content was 4.98% and 11.10%, and the extract content was between 7.95% and 21.40%. The water content of the whole herb is 7.450.12.24, the total ash is 5.0411.29 and the acid insoluble ash is 1.396.58%. The content of the extract was 24.96% and 38.05%; The water content of the aboveground part was 8.35 and 15.62, the total ash content was 3.94 and 5.00, the acid insoluble ash was 0.17% and 0.80%. There was no significant difference between the calculated results of multi-evaluation and that of external standard method, and under different experimental conditions, the content of extracts was 26.61% and 35.76% (5%), and there was no significant difference between the calculated results and the results obtained by external standard method. The reproducibility of the calibration factors among the components is good. Therefore, a method of multiple evaluation can be applied to the quality evaluation of Flower and Flower. 6) A common pattern of fingerprint of Flower and Flower is established. The 14 common peaks were calibrated, and the corresponding substances of four main chromatographic peaks were determined. Conclusion: the quality of medicinal materials of Chlorophyllum grandiflorum was preliminarily evaluated by the experiments and studies above, which is the safety of its clinical application. The research content of this paper has been confirmed by scientific and technological review, and there is no domestic and foreign literature report which is the same as the above research at present.
【學位授予單位】：廣西中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R29

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