本文關(guān)鍵詞：加味黃芪建中湯對(duì)消化性潰瘍的臨床與實(shí)驗(yàn)研究　出處：《延邊大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 加味黃芪建中湯 消化性潰瘍 臨床觀察 胃蛋白酶 一氧化氮

【摘要】：研究目的:本臨床療效觀察與動(dòng)物實(shí)驗(yàn)的目的主要是為了探討加味黃苗建中湯對(duì)順化性潰瘍治療作用及其機(jī)理。研究方法:1.選擇已經(jīng)確診為消化性潰瘍的患者60名又必須都符合中醫(yī)證型(脾胃虛寒證),60名患者是根據(jù)每位患者到醫(yī)院診療的日期與時(shí)間順序分為中藥治療組30名與西藥對(duì)照組30名。中藥治療組用加味黃苗建中湯(黃芪20g,飴糖20g,桂枝10g,芍藥15g,干姜5g,大棗5枚,炙甘草5g,白芨10g,小茴香10g)治療,西藥對(duì)照組采用奧美拉唑腸溶片(由江蘇聯(lián)環(huán)藥業(yè)股份有限公司生產(chǎn),批準(zhǔn)文號(hào):國(guó)藥準(zhǔn)字H10980267)治療。本研究以治療4周為一個(gè)療程,治療4周后比較分析兩組的臨床療效,中醫(yī)主要癥狀積分變化,出現(xiàn)的不良反應(yīng)。最后觀察所得數(shù)據(jù)利用SPSS22.0軟件包進(jìn)行統(tǒng)計(jì)分析。2.健康清潔級(jí)雄性SD小鼠,50只隨機(jī)分為5組,依次為:正常對(duì)照組、胃潰瘍模型組、西藥對(duì)照組(奧美拉唑組)、加味黃苗建中湯高劑量組、加味黃芪建中湯低劑量組,一個(gè)組放10只,分組飼養(yǎng)。正常組除外的每組均采用乙酸灌胃制造小鼠胃潰瘍模型。造模成功后24小時(shí)對(duì)所有小鼠恢復(fù)正常喂養(yǎng);對(duì)西藥對(duì)照組(奧美拉唑組)、加味黃芪建中湯高劑量組、加味黃芪建中湯低劑量組給予藥物的治療。持續(xù)治療3周后,小鼠禁食不禁水24小時(shí),采用摘除眼球采血的方法,采集的血液盡快離心后放置在-20℃的冰箱中保存。然后取出胃部,沿胃角切開(kāi)胃壁,放入裝有生理鹽水的器皿中在溫度為4℃的冰箱中冷藏保存。用肉眼觀察胃潰瘍的大體程度,檢測(cè)胃蛋白酶活力、血清一氧化氮(NO)的含量。最后用SPSS22.0統(tǒng)計(jì)軟件進(jìn)行統(tǒng)計(jì)分析實(shí)驗(yàn)數(shù)據(jù)。研究結(jié)果:1.臨床療效觀察結(jié)果:在臨床療效方面:中藥治療組總有效率為83.3%西藥對(duì)照組總有效率為86.7%,所以中藥治療組與西藥對(duì)照組的臨床療效無(wú)顯著性差異(P0.05),中藥治療組與西藥對(duì)照組臨床療效都很顯著。中醫(yī)臨床癥狀積分變化方面:中藥治療組的中醫(yī)癥狀積分降低程度與西藥對(duì)照組的中醫(yī)癥狀積分降低程度有顯著性差異(P0.05),中藥治療組的中醫(yī)癥狀積分降低程度更大。出現(xiàn)的不良反應(yīng):兩組治療期間出現(xiàn)的不良反應(yīng)次數(shù)比較有顯著性差異(P0.05),治療組治療期間不良反應(yīng)出現(xiàn)次數(shù)為5例,對(duì)照組治療期間不良反應(yīng)出現(xiàn)次數(shù)為12例。2.動(dòng)物實(shí)驗(yàn)結(jié)果:加味黃苗建中湯高劑量組與低劑量組和模型組比較時(shí)低劑量組的胃蛋白酶活力降低(P0.05),而高劑量組的胃蛋白酶活力顯著降低(P0.01);測(cè)血清一氧化氮(NO)中高低劑量組與模型組比較時(shí)高劑量組的一氧化氮(NO)增加(P0.05),而低劑量組的一氧化氮(NO)顯著增加(P0.01)。結(jié)論:臨床療效觀察:(1)加味黃苗建中湯在改善中醫(yī)癥狀積分方面優(yōu)于單純奧美拉唑的西藥對(duì)照組。(2)加味黃苗建中湯在不良反應(yīng)的出現(xiàn)方面優(yōu)于單純奧美拉唑的西藥對(duì)照組。動(dòng)物實(shí)驗(yàn):(1)加味黃芪建中湯各劑量組能降低模型小鼠胃蛋白酶活力,高劑量組與低劑量組相比時(shí)高劑量組的療效更佳。(2)加味黃芪建中湯各劑量組能增加模型小鼠血清一氧化氮含量,高劑量組與低劑量組相比時(shí)低劑量組的療效更佳。
[Abstract]:Objective: To study the clinical and animal experiment is aimed to explore the effect of Jiawei Decoction on the treatment effect of Huang Miaojian in hue ulcer and its mechanism. Methods: 1. selection has been diagnosed as peptic ulcer and 60 patients with TCM syndrome type must (spleen and stomach deficiency cold syndrome), 60 patients according to the date the diagnosis and treatment of each patient to the hospital and the time sequence is divided into Chinese medicine treatment group 30 and control group of Western medicine. 30 traditional Chinese medicine treatment group with modified yellow Miaojian Decoction (Radix Astragali 20g, maltose 20g, cinnamon 10g, peony 15g, 5g dried ginger, jujube 5 pieces, fried licorice 5g, Bletilla 10g, cumin 10g) treatment the western medicine group, with Omeprazole Enteric-coated Tablets (by Jiangsu pharamceutical Limited by Share Ltd production approval number: Zhunzi H10980267). In this study, treatment for 4 weeks for a course of treatment, after 4 weeks of treatment, compared the clinical efficacy of the two groups, mainly Chinese Medicine The symptoms integral change, adverse reactions. The last observed data package statistical analysis.2. healthy male SD mice by using SPSS22.0 software, 50 rats were randomly divided into 5 groups, respectively: normal control group, gastric ulcer model group, western medicine control group (omeprazole group), modified Huang Miaojian Decoction high dose group modified Huangqijianzhong Decoction low dose group, a group of 10, group feeding. Each group except for normal group were intragastric administration by acetic acid producing gastric ulcer in mice model. After the modeling 24 hours for all the mice returned to normal feeding; the western medicine control group (omeprazole group), modified Huangqijianzhong Decoction high dose modified Huangqijianzhong Decoction group, low dose group received drug therapy. After 3 weeks of continuous treatment, mice were fasted for 24 hours, using the method of removal of the eye blood, the blood collected as soon as possible after centrifugation, stored in the refrigerator. Then the -20 C Remove the stomach, the stomach angle cut into the stomach, with physiological saline at the temperature of refrigerated containers in the refrigerator 4 degrees. In observing the degree of gastric ulcer with the naked eye, the detection of pepsin activity, serum nitric oxide (NO) content. Finally using SPSS22.0 statistical software for statistical analysis of experimental data. Results: 1. results: the clinical curative effect in clinical curative effect: treatment group total effective rate was 83.3% in the western medicine group the total effective rate was 86.7%, so there was no significant difference between the clinical curative effect of traditional Chinese medicine treatment group and Western medicine control group (P0.05), Chinese medicine group and Western medicine group clinical curative effect is very notable. TCM clinical symptom score changes: TCM symptoms of Chinese medicine treatment group to reduce the degree of the symptom integral of traditional Chinese medicine and Western medicine control group had significant difference (P0.05), reduce the degree of TCM symptoms of Chinese medicine treatment group to reduce the process A greater degree of adverse reaction. The number of adverse reactions: during the treatment of the two groups had significant difference (P0.05), the treatment group adverse reaction occurred during times of 5 cases, control group treatment adverse reactions occurred during the number of 12 cases of.2. animal experimental results: modified Huang Miaojian Decoction high dose group and low dose group and model group when compared with low dose group of pepsin activity decreased (P0.05), and high dose group of pepsin activity decreased significantly (P0.01); serum nitric oxide (NO) level of nitric oxide in the dose group compared with the model group, high dose group (NO) increased (P0.05), and low dose group nitric oxide (NO) increased significantly (P0.01). Conclusion: the clinical observation: (1) Huang Miaojian Jiawei Decoction in improving the TCM symptom score than omeprazole control group of Western medicine. (2) modified Huang Miaojian Decoction in the adverse reaction. The surface is better than that of omeprazole control group of Western medicine. Animal experiment: (1) modified Huangqijianzhong Decoction each dose group can decrease the activity of pepsin in mice, the curative effect is better in high dose group compared with the low dose group, high dose group. (2) modified Huangqijianzhong Decoction each dose group can increase the content of serum nitric oxide model the mice in high dose group, low dose group and the curative effect is better when compared with the low dose group.

【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R259
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本文編號(hào)：1440027