發(fā)布時(shí)間：2018-01-12 14:04

  本文關(guān)鍵詞：疸清顆粒對(duì)肝內(nèi)膽汁於積大鼠治療的實(shí)驗(yàn)研究　出處：《遼寧中醫(yī)藥大學(xué)》2016年碩士論文　論文類(lèi)型：學(xué)位論文

  更多相關(guān)文章： 疸清顆粒 肝內(nèi)膽汁淤積 病證結(jié)合

【摘要】：目的:觀察疸清顆粒對(duì)于α-萘基異硫氰酸鹽(ANIT)誘導(dǎo)具有濕熱證候肝內(nèi)膽汁淤積型大鼠的療效,分析并探討其機(jī)制。材料與方法:將60只健康清潔級(jí)Wistar大鼠隨機(jī)分為空白對(duì)照組10只,以及模型組50只(模型1組、模型2組、茵梔黃顆粒組、疸清顆粒低劑量組、疸清顆粒高劑量組,每組各10只)�？瞻讓�(duì)照組每日予以0.9%生理鹽水灌胃,將剩余所有大鼠放入濕熱造模箱內(nèi),并每日給予50%葡萄糖和豬油脂混合液灌胃,連續(xù)3周,模擬中醫(yī)濕熱證候。第22日將造模組50只大鼠一次性給予2%α-萘基異硫氰酸鹽溶液灌胃,復(fù)制具有濕熱證候急性肝內(nèi)膽汁淤積大鼠模型。模型1組造模后48h處死,留取血清、膽汁等標(biāo)本備用,光鏡下觀察肝組織切片,成功建立模型。模型2組于造模當(dāng)天起日一次予以0.9%生理鹽水灌胃;剩余組別大鼠均給與相應(yīng)藥物灌胃給藥,連續(xù)7天。第29日處死所有大鼠,采集標(biāo)本。觀察不同藥物對(duì)模型大鼠肝功能、膽汁成分、肝臟脂蛋白X的影響,光鏡下觀察肝組織病理學(xué)改變,電鏡觀察肝細(xì)胞超微結(jié)構(gòu)。結(jié)果:1.與空白組比較,兩模型組大鼠一般生理狀態(tài)較差;各血清生化指標(biāo)(ALT、AST、ALP、TBIL、DBIL、TBA)升高明顯(P0.05);脂蛋白X升高明顯(P0.05);膽汁流量減少,流速減慢(P0.05),膽汁中LEC、TC降低,TBA、TB升高,均有統(tǒng)計(jì)學(xué)意義(P0.05);光鏡下可見(jiàn)肝細(xì)胞腫脹、排列層次紊亂,可見(jiàn)大量炎性細(xì)胞和脂肪細(xì)胞;電鏡下可見(jiàn)細(xì)胞質(zhì)內(nèi)線粒體腫脹破裂,呈空泡樣改變。2.模型2組與模型1組比較,血清中ALT、AST、ALP、TBIL、DBIL、TBA水平和脂蛋白X含量均有所降低(P0.05);鏡下肝組織損傷程度接近。3.與模型2組比較,三組治療組大鼠一般狀態(tài)好轉(zhuǎn),各血清指標(biāo)以及脂蛋白均有明顯下降(P0.05);膽汁流量增多,流速加快(P0.05),膽汁中LEC、TC顯著升高,TBA、TB降低明顯,均有統(tǒng)計(jì)學(xué)意義(P0.05);鏡下肝組織及超微結(jié)構(gòu)有明顯好轉(zhuǎn)。4.與茵梔黃組比較,疸清顆粒高低劑量組大鼠一般狀態(tài)略好,血清中TBIL、DBIL、TBA明顯降低(P0.05);鏡下肝組織及超微結(jié)構(gòu)恢復(fù)程度更好一些。5.疸清顆粒高低劑量組各個(gè)實(shí)驗(yàn)指標(biāo)均無(wú)明顯差異(P0.05),鏡下肝組織及超微結(jié)構(gòu)基本相同。結(jié)論:1.疸清顆�？梢愿纳茲駸嶙C候肝內(nèi)膽汁淤積大鼠的生理狀態(tài)。2.疸清顆�？梢愿纳聘喂δ�、促進(jìn)膽汁分泌排泄并減輕肝細(xì)胞病變及壞死。3.疸清顆粒對(duì)濕熱證候肝內(nèi)膽汁淤積癥有積極的治療作用。
[Abstract]:Objective: to observe the therapeutic effect of Jieqing granule on 偽 -naphthyl isothiocyanate ANIT-induced intrahepatic cholestasis of rats with damp-heat syndrome. Materials and methods: 60 healthy and clean grade Wistar rats were randomly divided into blank control group (n = 10) and model group (n = 50) (model 1, model 2). Yinzhihuang granule group, jinjiqing granule low dose group, jinjiqing granule high dose group, each group 10 rats. Blank control group was given 0.9% normal saline daily, the remaining rats were put into the damp-heat modeling box. In 22nd, 50 rats in the model group were given 2% 偽 -naphthyl isothiocyanate solution in a single dose of 2% 偽 -naphthyl isothiocyanate solution. The rat model of acute intrahepatic cholestasis with dampness and heat syndrome was established. The model group 1 was killed 48 hours after the model was made. The serum and bile were collected and the liver tissue sections were observed under light microscope. The model was established successfully. Group 2 was given 0.9% normal saline once a day from the same day. On 29th, all the rats were killed and the samples were collected. The effects of different drugs on liver function, bile composition and liver lipoprotein X were observed in the model rats. The pathological changes of liver tissue were observed under light microscope and the ultrastructure of hepatocytes were observed by electron microscope. Results: compared with the blank group, the general physiological state of the two model groups was worse than that of the control group. The serum biochemistry index (ALT) was significantly higher than that of the control group (P 0.05), and the level of TBA was significantly higher than that of the control group (P < 0.05). Lipoprotein X increased significantly (P 0.05); The volume of bile decreased, the velocity of flow slowed down (P 0.05), and the level of LEC-TC in bile decreased the increase of TBATB, all of which had statistical significance (P 0.05). Under the light microscope, the liver cells were swollen and arranged in disorder, and a large number of inflammatory cells and adipocytes could be seen. Under electron microscope, mitochondria in the cytoplasm were swollen and ruptured, showing vacuolar changes. Compared with model 1, the serum levels of alt ASTN and TBIL-DBIL in model 2 group were higher than those in model 1 group (P < 0.05). The level of TBA and the content of lipoprotein X decreased P0.05; The degree of liver injury was close to .3.Compared with the model group 2, the general state of the rats in the three treatment groups was improved, and the serum indexes and lipoproteins were significantly decreased (P 0.05). The bile flow rate increased, the velocity of flow increased, and the LEC-TC in bile increased significantly and TBATTB decreased significantly, all of which had statistical significance (P 0.05). Compared with the Yinzhihuang group, the rats in the high and low dosage group of jinjiqing granule were in good general condition, and the serum TBIL-DBIL was better than that in the control group. TBA decreased significantly (P 0.05); The degree of recovery of liver tissue and ultrastructure was better under microscope. There was no significant difference in each experimental index between high and low dosage groups of jinjiqing granules (P0.05). The liver tissue and ultrastructure were basically the same under the microscope. Conclusion 1. Jinjiqing granule can improve the physiological state of intrahepatic cholestasis rats with damp-heat syndrome .2. jinjiqing granule can improve liver function. Promoting bile excretion and reducing hepatocyte lesion and necrosis .3.Jinjiqing granule has a positive therapeutic effect on intrahepatic cholestasis of damp-heat syndrome.
【學(xué)位授予單位】：遼寧中醫(yī)藥大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類(lèi)號(hào)】：R259
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本文編號(hào)：1414557