本文關鍵詞：龜板聯(lián)合阿倫磷酸鈉對激素性骨質疏松大鼠腰椎Runx2、CTSK表達的影響　出處：《中華中醫(yī)藥雜志》2017年05期 　論文類型：期刊論文

  更多相關文章： 激素性骨質疏松 龜板 阿倫磷酸鈉 協(xié)同作用 CTSK Runx

【摘要】：目的:觀察龜板聯(lián)合阿倫磷酸鈉(ALN)對激素性骨質疏松(GIOP)大鼠腰椎Runx2、CTSK表達的影響。方法:40只3月齡雌性SD大鼠隨機分為4組:空白組、模型組、ALN組、龜板聯(lián)合ALN組(聯(lián)合組)。模型組、ALN組及聯(lián)合組皮下注射地塞米松造模,成功后分別用0.9%氯化鈉溶液、ALN和龜板聯(lián)合ALN進行灌胃。12周后取大鼠腰椎進行:micro-CT檢測腰椎骨微細結構、HE染色觀察骨組織形態(tài)學,qPCR及Western blot分別檢測腰椎Runx2、CTSK mRNA和蛋白表達。結果:與空白組比較,模型組Tb.N、Tb.Th明顯下降(P0.01),Tb.Sp、CTSK mRNA和蛋白表達明顯升高(P0.01),Runx2 mRNA和蛋白表達呈下調趨勢;與模型組比較,ALN組和聯(lián)合組BS/TV、Tb.N、Tb.Th、Runx2蛋白表達明顯升高(P0.01),Tb.Sp、CTSK mRNA和蛋白表達明顯下調(P0.01)。結論:龜板聯(lián)合阿倫磷酸鈉抗大鼠腰椎GIOP的協(xié)同機制可能與調節(jié)Runx2、CTSK的表達密切相關。
[Abstract]:Objective: To observe the effect of Plastrum Testudinis combined with Allen sodium phosphate (ALN) on glucocorticoid induced osteoporosis (GIOP) rats lumbar Runx2, CTSK expression. Methods: 40 March old female SD rats were randomly divided into 4 groups: control group, model group, ALN group, combined group ALN (combined group). The model of turtle shell group, ALN group and combined group subcutaneous injection of dexamethasone model, after the success of Sodium Chloride Solution ALN and 0.9% respectively, combined with ALN.12 by gavage Testudinis weeks after rat lumbar micro-CT detection of lumbar bone micro structure of bone tissue morphology, HE staining, qPCR and Western blot respectively to detect expression of CTSK mRNA Runx2 of the lumbar spine. And protein. Results: compared with the control group, Tb.N model group, Tb.Th decreased significantly (P0.01), Tb.Sp, mRNA and expression of CTSK protein increased significantly (P0.01), the expression of Runx2 mRNA and protein decreased; compared with the model group, ALN group and combined group BS/TV, Tb.N, Tb.Th, Runx2 protein expression in Ming Dynasty Xian Shenggao (P0.01), Tb.Sp, mRNA and protein expression of CTSK was significantly reduced (P0.01). Conclusion: the coordination mechanism of sodium phosphate in rats with Allen turtle lumbar GIOP may be related to the regulation of Runx2, the expression of CTSK is closely related.

【作者單位】： 廣州中醫(yī)藥大學;廣州中醫(yī)藥大學第一附屬醫(yī)院;
【基金】：國家自然科學基金項目(No.81503591,No.81674000) 廣東省科技廳項目(No.2014 A020221021,No.2016A020226006) 廣東省自然科學基金項目(No.2014A030310082,No.2016A030313645) 廣州中醫(yī)藥大學及第一臨床醫(yī)學院優(yōu)秀博士論文培育項目(No.Y B201501,No.Y B201602);廣州中醫(yī)藥大學優(yōu)秀青年學者科研基金項目(No.KAB110133K04) 廣州中醫(yī)藥大學第一附屬醫(yī)院創(chuàng)新強院項目(No.2015Q N03) 廣東省教育廳學科建設專項基金(育苗工程)(No.2013LYM-0012)~~
【分類號】：R245
【正文快照】： 糖皮質激素性骨質疏松癥(glucocor ticoid-inducedosteoporosis,GIOP)是最常見繼發(fā)性骨質疏松癥,是藥用糖皮質激素(glucocorticoid,GC)常見不良反應之一,其防治和機制研究引起國內外學者普遍重視[1]。二磷酸鹽可抑制骨重建、提高骨強度、降低骨折率,是GIOP一線用藥[2],但其具，

本文編號：1360318