【摘要】：目的:研究非小細(xì)胞肺癌(non-small cell lung cancer,NSCLC)細(xì)胞A549與小鼠腦血管內(nèi)皮細(xì)胞b.End.3共培養(yǎng)對b.End.3中Epsin1以及其他相關(guān)因子表達(dá)量的影響。方法:使用b.End.3小鼠腦血管內(nèi)皮細(xì)胞與非小細(xì)胞肺癌A549細(xì)胞建立體外血腦屏障模型(blood brain barrier,BBB)、血瘤屏障(blood tumor barrier,BTB)模型。實驗分為兩組:BBB組、BTB組。使用流式細(xì)胞儀分選BTB組中的b.End.3細(xì)胞。使用q PCR和Western Blot的方法分別檢測分選出的b.End.3細(xì)胞中Epsin1、CD309、CD144、Occludin轉(zhuǎn)錄和表達(dá)水平的變化。結(jié)果:與A549細(xì)胞共培養(yǎng)后,BTB組跨膜細(xì)胞電阻值(transendothelial electrical resistance,TEER)較BBB組下降(P0.05)。共培養(yǎng)后分選出的b.End.3細(xì)胞中的Epsin1和CD309、CD144的表達(dá)量增高(P0.05),Occludin的表達(dá)量下降(P0.05)。結(jié)論:A549共培養(yǎng)后體外血腦屏障的開放可能與Epsin1及相關(guān)因子的表達(dá)增高有關(guān)。
[Abstract]:Aim: to study the effect of co-culture of non-small cell lung cancer (non-small cell lung cancer,NSCLC) cell line A549 and mouse cerebrovascular endothelial cell b.End.3 on the expression of Epsin1 and other related factors in b.End.3. Methods: the blood-brain barrier model (blood brain barrier,BBB) and blood-tumor barrier (blood tumor barrier,BTB) model were established by using b.End.3 mouse cerebral vascular endothelial cells and non-small cell lung cancer cell line A549 cells in vitro. The experiment was divided into two groups: BBB group and BTB group. B.End.3 cells in BTB group were separated by flow cytometry. Q PCR and Western Blot methods were used to detect the changes of Epsin1,CD309,CD144,Occludin transcription and expression in the selected b.End.3 cells. Results: after co-culture with A549 cells, the resistance value of transmembrane cells in BTB group was lower than that in BBB group (P 0.05). After co-culture, the expression of Epsin1 and CD309,CD144 in b.End.3 cells increased (P 0.05), and the expression of), Occludin decreased (P 0.05). Conclusion: the opening of blood-brain barrier in vitro after co-culture of A549 may be related to the increased expression of Epsin1 and related factors.
【作者單位】： 昆明醫(yī)科大學(xué)第一附屬醫(yī)院神經(jīng)外科;昆明醫(yī)科大學(xué)生物化學(xué)與分子生物學(xué)系;
【基金】：云南省科技廳-昆明醫(yī)科大學(xué)應(yīng)用基礎(chǔ)研究聯(lián)合專項資金(2015FB033)
【分類號】：R734.2
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本文編號：2519521