【摘要】：目的叉頭框轉(zhuǎn)錄因子P3(forkhead box P3,FOXP3)屬于叉頭框/翼狀螺旋轉(zhuǎn)錄因子(forkhead box,FOX)家族成員,早期被認(rèn)為特異性表達(dá)于免疫抑制性CD4+CD25+調(diào)節(jié)性T細(xì)胞(CD4+CD25+regulatory T cell,Treg)。而近些年來研究發(fā)現(xiàn),FOXP3在多種腫瘤細(xì)胞中均有表達(dá)。本研究旨在探討FOXP3在乳腺浸潤性導(dǎo)管癌組織中的表達(dá)與臨床病理學(xué)特征的關(guān)系及其預(yù)后意義。方法收集2009-01-01-2012-04-30河北醫(yī)科大學(xué)第四醫(yī)院乳腺中心收治的123例乳腺浸潤性導(dǎo)管癌標(biāo)本,采用免疫組織化學(xué)法檢測FOXP3蛋白的表達(dá),分析FOXP3與腫瘤臨床病理學(xué)特征間的關(guān)系,并采用Kaplan-Meier法及Cox比例回歸風(fēng)險模型進(jìn)行生存分析。結(jié)果 FOXP3蛋白在乳腺浸潤性導(dǎo)管癌實(shí)質(zhì)細(xì)胞質(zhì)和細(xì)胞核中均有表達(dá),FOXP3總表達(dá)率為68.29%(84/123)。生存分析結(jié)果顯示,FOXP3表達(dá)陽性組的無病生存率(disease free survival,DFS)為89.29%,高于陰性組的71.79%,差異有統(tǒng)計學(xué)意義,χ~2=6.119,P=0.013;但2組的總生存率(overall survival,OS)差異無統(tǒng)計學(xué)意義,χ~2=1.911,P=0.167。進(jìn)一步分析FOXP3在乳腺癌細(xì)胞中的表達(dá)部位發(fā)現(xiàn),FOXP3在細(xì)胞核中表達(dá)率為47.97%(59/123),細(xì)胞質(zhì)中為63.41%(78/123)。生存分析結(jié)果顯示,FOXP3細(xì)胞核表達(dá)陽性組的OS和DFS分別為94.92%和91.53%,均高于細(xì)胞核陰性組的82.81%和76.56%,差異均有統(tǒng)計學(xué)意義,χ~2值分別為5.265和4.974,P值分別為0.022和0.026;且Cox多因素分析結(jié)果顯示,細(xì)胞核FOXP3是改善OS的獨(dú)立預(yù)后因素,HR=0.245,P=0.033;但細(xì)胞質(zhì)FOXP3與預(yù)后無明顯相關(guān)性。在FOXP3細(xì)胞核表達(dá)陽性患者中,無脈管瘤栓組(χ~2=5.117,P=0.024)及Ki-67低表達(dá)組(χ~2=4.214,P=0.041)的表達(dá)率更高;且各分子分型間表達(dá)率差異有統(tǒng)計學(xué)意義,χ~2=12.983,P=0.002;在Luminal A型乳腺癌中FOXP3細(xì)胞核表達(dá)率最高,為68.18%。結(jié)論FOXP3在乳腺浸潤性導(dǎo)管癌中的預(yù)后意義與表達(dá)部位相關(guān),細(xì)胞核FOXP3高表達(dá)是改善乳腺癌OS的獨(dú)立預(yù)后因素,而細(xì)胞質(zhì)FOXP3的表達(dá)意義尚不明確。細(xì)胞核FOXP3可作為乳腺癌預(yù)后良好的預(yù)測指標(biāo)。
[Abstract]:Objective the fork frame transcription factor P3 (forkhead box P3, which belongs to the forkhead frame / pterygoid spiral transcription factor (forkhead box,FOX) family, is thought to be specifically expressed in immunosuppressive CD4 CD25 regulatory T cells (CD4 CD25 regulatory T cell,Treg) at an early stage. In recent years, it has been found that FOXP3 is expressed in a variety of tumor cells. The purpose of this study was to investigate the relationship between the expression of FOXP3 and clinicopathological features in invasive ductal carcinoma of breast and its prognostic significance. Methods 123 cases of invasive ductal carcinoma of breast treated in the fourth Hospital of Hebei Medical University in 2009 / 01 / 01 / 2012 / 02 / 30 were collected. The expression of FOXP3 protein was detected by immunohistochemistry. The relationship between FOXP3 and clinicopathological features of the tumor was analyzed, and the survival analysis was carried out by Kaplan-Meier method and Cox proportional regression risk model. Results FOXP3 protein was expressed in both cytoplasm and nucleus of invasive ductal carcinoma of breast. The total expression rate of FOXP3 was 68.29% (84 鈮，

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