【摘要】：目的探討離心力對(duì)質(zhì)譜流式細(xì)胞技術(shù)檢測(cè)結(jié)腸癌細(xì)胞表面抗原CD45表達(dá)結(jié)果的影響。方法將體外培養(yǎng)的結(jié)腸癌HCT116細(xì)胞分為400 g離心組和140 g離心組(每組細(xì)胞數(shù)均為3×10~6個(gè)),分別采用400 g、140 g離心力離心5 min,重復(fù)兩次,收集細(xì)胞。采用質(zhì)譜流式細(xì)胞技術(shù)檢測(cè)兩組死亡細(xì)胞百分比、抗人CD45金屬聚合抗體(154Sm)信號(hào)強(qiáng)度10~2的細(xì)胞數(shù)占總活細(xì)胞數(shù)的比例、t-SNE圖顯示活細(xì)胞中高表達(dá)CD45的細(xì)胞數(shù)量。結(jié)果 400 g離心力組及140 g離心力組死亡細(xì)胞百分比分別為(7.69±0.7)%、(19.06±0.66)%,兩組比較P0.01。400 g離心力組及140 g離心力組154Sm信號(hào)強(qiáng)度10~2的細(xì)胞數(shù)分別占活細(xì)胞總數(shù)的0.2%[(0.19±0.08)%]、2.9%[(2.88±0.03)%],兩組比較P0.01。t-SNE圖顯示,400 g離心力組及140 g離心力組CD45高表達(dá)細(xì)胞分別為(167.41±70.48)、(201.20±20.96)個(gè),兩組比較P0.01。結(jié)論應(yīng)用質(zhì)譜流式細(xì)胞技術(shù)檢測(cè)結(jié)腸癌細(xì)胞表面抗原CD45表達(dá)時(shí),140 g離心力分離的細(xì)胞檢測(cè)結(jié)果更好。
[Abstract]:Objective to investigate the effect of centrifugal force on the expression of surface antigen CD45 in colon cancer cells by mass spectrometry flow cytometry. Methods Colon cancer HCT116 cells cultured in vitro were divided into 400g centrifugation group and 140g centrifugation group (the number of cells in each group was 3 脳 10 脳 6). The cells were collected twice with 400g centrifugation and 140g centrifugal force centrifugation 5 min,. Mass spectrometry flow cytometry was used to detect the percentage of dead cells in the two groups. The number of cells with 10 鈮，

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