【摘要】：原發(fā)性肝癌是世界上最常見的惡性腫瘤之一,同時在惡性腫瘤中,原發(fā)性肝癌致死率排名第三。據統(tǒng)計,在2008年,每年大概有700000人被診斷為原發(fā)性肝癌,其發(fā)病率為每100,000人中就有16人患有原發(fā)性肝癌。對于早期患者,我們可以選擇肝臟腫瘤切除,肝移植及射頻消融等手段進行治療。然而高復發(fā)率是影響早期肝癌患者生存的主要障礙,在臨床上,我們常用一些臨床指標來預測患者治療后的效果,其中包括腫瘤大小,血管侵潤,AFP及病理分級等。然而,許多分子標志物來預測肝癌的預后還沒有被公認,因此,積極尋找有效的分子標志物對于研究原發(fā)性的預后有著重要的意義。FOX家族是一個具有高保真的DNA區(qū)域和組織特異性表達的特點,其在調控細胞的生長分化,胚胎的形成及發(fā)育有著重要的作用。FOXF2作為FOX家族中的一員,已經被證實在乳腺癌及前列腺癌的發(fā)生發(fā)展中有著重要的作用。然而,其在肝癌中的表達及預后關系尚未有相關文獻報道。[目的]本課題以肝癌為研究對象,選擇FOXF2分子,檢測在肝癌中的表達水平及與預后的關系,并在肝癌細胞系研究其在細胞水平上的表達與細胞的生物學行為的關系。[方法](1)利用免疫組化方法和real-time PCR檢測295例肝癌及配對癌旁組織FOXF2表達并與臨床病理學參數(shù)進行相關性分析。(2)利用IHC、Western Blot和real-time PCR方法檢測295例肝癌組織和細胞的FOXF2表達并與臨床病理學參數(shù)進行相關性分析。(3)通過人工合成FOXF2的干擾片,干涉MHCC-97H肝癌細胞系FOXF2的表達,檢測其對肝癌細胞增殖、凋亡的影響。[結果]1.(1)通過IHC、Western Blot和real-time PCR技術檢測結果,我們發(fā)現(xiàn)FOXF2在肝癌組織中的表達水平比癌旁低。2.(1) FOXF2在肝癌組織表達下降,另外在轉移能力和侵襲能力較強的肝癌細胞系MHCC-97H中表達下降；(2)經統(tǒng)計分析結果顯示FOXF2的低表達與肝癌患者的較差的預后呈正相關性；(3)關于FOXF2在肝癌細胞系中表達的初步功能研究發(fā)現(xiàn)FOXF2表達促進細胞的凋亡和抑制肝癌細胞的增殖；[結論]1.FOXF2表達下降在肝癌的發(fā)生發(fā)展中可能起重要作用,FOXF2的表達可作為肝癌患者術后生存的獨立預測指標。2. FOXF2表達下降可提高肝癌細胞的抗凋亡能力并促進肝癌細胞的增殖。
[Abstract]:Primary liver cancer is one of the most common malignant tumors in the world, and the mortality rate of primary liver cancer ranks third among malignant tumors. According to statistics, in 2008, about 700000 people are diagnosed with primary liver cancer every year, and the incidence rate is 16 out of every 100000 people with primary liver cancer. For early patients, we can choose liver tumor resection, liver transplantation and radiofrequency ablation. However, high recurrence rate is the main obstacle to the survival of patients with early liver cancer. Clinically, we often use some clinical indicators to predict the effect of patients after treatment, including tumor size, vascular invasion, AFP and pathological grade. However, many molecular markers to predict the prognosis of liver cancer have not been recognized, so it is of great significance to actively find effective molecular markers for the study of primary prognosis. Fox family is a characteristic of high fidelity DNA region and tissue specific expression, which plays an important role in regulating cell growth and differentiation, embryo formation and development. FOXF2, as a member of FOX family, It has been proved to play an important role in the occurrence and development of breast cancer and prostate cancer. However, the relationship between its expression and prognosis in HCC has not been reported in the literature. [objective] in this study, FOXF2 molecule was selected to detect the expression level of HCC and its relationship with prognosis, and the relationship between the expression of HCC molecule and the biological behavior of HCC cell line was studied. [methods] (1) Immunohistochemical method and real-time PCR were used to detect the expression of FOXF2 in 295 cases of HCC and matched paracancerous tissues and their correlation with clinicopathological parameters. (2) the expression of FOXF2 in 295 cases of HCC tissues and cells was detected by IHC,Western Blot and real-time PCR, and the correlation between FOXF2 expression and clinicopathological parameters was analyzed. (3) the expression of FOXF2 in MHCC-97H HCC cell line was interfered with by synthetic FOXF2 interference. To detect the effect of HCC on proliferation and apoptosis of HCC cells. [results] 1. (1) the expression level of FOXF2 in HCC tissues was lower than that in HCC tissues by IHC,Western Blot and real-time PCR. (1) the expression of FOXF2 in HCC tissues decreased, in addition, in HCC cell line MHCC-97H with strong metastasis and invasiveness. (2) the results of statistical analysis showed that the low expression of FOXF2 was positively correlated with the poor prognosis of HCC patients. (3) the preliminary study on the expression of FOXF2 in HCC cell line found that the expression of FOXF2 promoted the apoptosis of HCC cells and inhibited the proliferation of HCC cells. [conclusion] the decrease of 1.FOXF2 expression may play an important role in the occurrence and development of HCC. The expression of FOXF2 can be used as an independent predictor of postoperative survival in patients with HCC. 2. The decrease of FOXF2 expression can improve the anti-apoptosis ability of HCC cells and promote the proliferation of HCC cells.
【學位授予單位】：蘇州大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R735.7

【參考文獻】

相關期刊論文 前1條

1 ;Expression and clinical significance of TAp73α, p53, PCNA and apoptosis in hepatocellular carcinoma[J];World Journal of Gastroenterology;2005年18期

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本文編號：2499383