【摘要】：目的:明確卡波西肉瘤組織中血管內(nèi)皮生長因子A(VEGFA)的表達(dá)情況,探討VEGFA的表達(dá)與卡波西肉瘤臨床病理特征之間的關(guān)系。方法:選取新疆地區(qū)30例卡波西肉瘤石蠟組織、21例血管瘤石蠟組織及15例正常皮膚石蠟組織,分別采用實(shí)時熒光定量PCR及免疫組化兩種方法檢測三種石蠟組織中VEGFA m RNA及蛋白的表達(dá)水平。結(jié)果:VEGFA m RNA在卡波西肉瘤組織中的表達(dá)水平為11.67±1.80,在血管瘤組織中的表達(dá)水平為10.90±1.22,兩組比較差異無統(tǒng)計學(xué)意義(P0.05);VEGFA m RNA在正常皮膚組織的表達(dá)水平為1.02±0.20,卡波西肉瘤組織中VEGFA m RNA的表達(dá)高于正常皮膚組織,差異具有統(tǒng)計學(xué)意義(P0.05);VEGFA蛋白在卡波西肉瘤組織、血管瘤組織、正常皮膚組織中的陽性表達(dá)率分別為83.3%、71.4%、6.7%,VEGFA蛋白在卡波西肉瘤組織中的表達(dá)與正常皮膚組織比較,差異具有統(tǒng)計學(xué)意義(P0.05),但與血管瘤組織比較無明顯差異(P0.05);VEGFA的表達(dá)與卡波西肉瘤患者年齡、性別、HHV-8感染、HIV感染、皮損面積差異無統(tǒng)計學(xué)意義(P0.05),而與病理分期呈正相關(guān)(rs=0.918,P=0.000),即結(jié)節(jié)期高于斑片期、斑塊期,斑塊期高于斑片期,差異有統(tǒng)計學(xué)意義(P0.05)。結(jié)論:VEGFA在卡波西肉瘤組織中的表達(dá)明顯升高,且與病理分期呈正相關(guān),提示VEGFA可能與卡波西肉瘤血管的發(fā)生、發(fā)展有密切關(guān)系,這可以為卡波西肉瘤的發(fā)病機(jī)制提供一個新的研究方向。
[Abstract]:Objective: to investigate the expression of vascular endothelial growth factor A (VEGFA) in Kaposi's sarcomas and to explore the relationship between the expression of VEGFA and the clinicopathological features of Kaposi's sarcomas. Methods: paraffin tissue of 30 cases of Kaposi's sarcomas, 21 cases of hemangioma and 15 cases of normal skin paraffin tissue were selected in Xinjiang. The expression of VEGFA m RNA and protein in three kinds of paraffin tissues were detected by real-time fluorescence quantitative PCR and immunohistochemistry, respectively. Results the expression level of: VEGFA m RNA in Kaposi's sarcomas was 11.67 鹵1.80, and that in hemangioma was 10.90 鹵1.22. There was no significant difference between the two groups (P 0.05). The expression level of VEGFA m RNA in normal skin tissue was 1.02 鹵0.20. the expression of VEGFA m RNA in Kaposi's sarcomas was higher than that in normal skin tissue, the difference was statistically significant (P 0.05). The positive expression rates of VEGFA protein in Kaposi's sarcomas, angiomas and normal skin tissues were 83. 3%, 71. 4% and 6. 7%, respectively. the expression of VEGFA protein in Kaposi's sarcomas was significantly higher than that in normal skin tissues. The difference was statistically significant (P 0.05), but there was no significant difference between the two groups (P 0.05). The expression of VEGFA was not significantly different from age, sex, HHV-8 infection, HIV infection and skin lesion area in patients with Kaposi's sarcomas (P 0.05), but positively correlated with pathological stage (rs=0.918,P=0.000), that is, nodular stage was higher than plaque stage. Plaque stage, plaque stage was higher than plaque stage, the difference was statistically significant (P 0.05). Conclusion: the expression of VEGFA in Kaposi's sarcomas is significantly increased, which is positively correlated with pathological stage, suggesting that VEGFA may be closely related to the occurrence and development of Kaposi's sarcomas. This can provide a new research direction for the pathogenesis of Kaposi's sarcomas.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R730.26

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 湯國雄;李琴;陶學(xué)金;朱聲榮;周磊磊;謝三祥;;Cosmc、T抗原和VEGFR/KDR在嬰兒血管瘤不同分期的表達(dá)及意義[J];臨床口腔醫(yī)學(xué)雜志;2014年10期

2 董宏超;翟校楓;;VEGFA-VEGFR2相關(guān)信號蛋白作用機(jī)制的研究進(jìn)展[J];現(xiàn)代腫瘤醫(yī)學(xué);2014年09期

3 殷芳;吳飛;陳佳;章楚光;王萍;宋寧靜;;TAMs在皮膚惡性黑素瘤組織中的浸潤及與STAT3、VEGF蛋白表達(dá)的關(guān)系[J];中華全科醫(yī)學(xué);2013年06期

4 劉桂君;陳國江;黎燕;石艷春;;血管內(nèi)皮生長因子在腫瘤生長轉(zhuǎn)移中的作用機(jī)制研究進(jìn)展[J];國際藥學(xué)研究雜志;2012年05期

5 周艷梅;江從軍;張汝芝;柴繼俠;賀文欣;陳曉宇;陳曉蓉;;NF-κB特異性抑制劑PDTC對鼠黑素瘤B16細(xì)胞增殖及VEGF表達(dá)的影響[J];蚌埠醫(yī)學(xué)院學(xué)報;2012年09期

6 張金玲;王s，

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