【摘要】：目的羅勒多糖具有抗腫瘤侵襲轉移、化療增效以及降血糖、降血脂和抗血栓等多種生物活性作用。本研究探討羅勒多糖對轉化生長因子-β(transforming growth factor-beta,TGF-β)誘導的人肺癌A549細胞侵襲運動能力的抑制作用,揭示羅勒多糖抑制上皮間質轉化作用的可能機制。方法體外培養(yǎng)肺癌A549細胞,實驗分為3組:(1)正常對照組,不加任何處理;(2)模型組,TGF-β5ng/mL;(3)羅勒多糖組,TGF-β5ng/mL+羅勒多糖200μg/mL。免疫熒光檢測羅勒多糖對E-cadherin、Vimentin和α-SMA表達的影響,細胞侵襲實驗和運動實驗檢測細胞對人工基底膜的降解和運動能力,細胞劃痕實驗檢測細胞運動能力。結果與正常對照組比較,模型組細胞Vimentin和α-SMA表達增強,E-cadherin表達減弱。羅勒多糖處理后Vimentin和α-SMA表達減弱,E-cadherin表達增強。TGF-β可提高細胞降解人工基底膜的能力,單位視野下侵襲至下室的細胞數(shù)模型組為69.0±4.3,與正常對照組(42.0±4.30)和羅勒多糖組(37.0±4.41)比較,差異有統(tǒng)計學意義,均P0.05。TGF-β增強了細胞的侵襲能力,羅勒多糖可以抑制TGF-β誘導的A549細胞侵襲能力。TGF-β使FN趨化的遷移細胞數(shù)目增多,單位視野下侵襲至下室的細胞數(shù)模型組為95.2±3.83,與正常對照組(69.2±5.11)和羅勒多糖組(68.2±6.18)比較,差異均有統(tǒng)計學意義,均P0.05。劃痕實驗顯示,TGF-β增強了細胞的遷移能力,羅勒多糖可以抑制TGF-β誘導的A549細胞遷移能力。結論羅勒多糖能夠調(diào)節(jié)A549細胞上皮間質轉化,這可能是其抑制A549細胞的侵襲轉移能力的重要機制。
[Abstract]:Objective basil polysaccharide has many biological activities, such as anti-tumor invasion and metastasis, chemotherapy synergy, hypoglycemia, blood lipid reduction and antithrombosis. The aim of this study was to investigate the inhibitory effect of basil polysaccharide on the invasiveness and motility of human lung cancer cell line A549 induced by transforming growth factor-尾 (transforming growth factor-beta,TGF- 尾 (transforming growth factor-beta,TGF- 尾), and to reveal the possible mechanism of basil polysaccharide inhibiting epithelial stroma transformation. Methods Lung cancer A549 cells were cultured in vitro and divided into three groups: (1) normal control group without any treatment; (2) model group, TGF- 尾 5 ng/mL; (3) basil polysaccharide group, TGF- 尾 5 ng/mL basil polysaccharide 200 渭 g / mL.. The effects of basil polysaccharide on the expression of E 鈮，

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