【摘要】：目的羅勒多糖具有抗腫瘤侵襲轉(zhuǎn)移、化療增效以及降血糖、降血脂和抗血栓等多種生物活性作用。本研究探討羅勒多糖對(duì)轉(zhuǎn)化生長因子-β(transforming growth factor-beta,TGF-β)誘導(dǎo)的人肺癌A549細(xì)胞侵襲運(yùn)動(dòng)能力的抑制作用,揭示羅勒多糖抑制上皮間質(zhì)轉(zhuǎn)化作用的可能機(jī)制。方法體外培養(yǎng)肺癌A549細(xì)胞,實(shí)驗(yàn)分為3組:(1)正常對(duì)照組,不加任何處理;(2)模型組,TGF-β5ng/mL;(3)羅勒多糖組,TGF-β5ng/mL+羅勒多糖200μg/mL。免疫熒光檢測羅勒多糖對(duì)E-cadherin、Vimentin和α-SMA表達(dá)的影響,細(xì)胞侵襲實(shí)驗(yàn)和運(yùn)動(dòng)實(shí)驗(yàn)檢測細(xì)胞對(duì)人工基底膜的降解和運(yùn)動(dòng)能力,細(xì)胞劃痕實(shí)驗(yàn)檢測細(xì)胞運(yùn)動(dòng)能力。結(jié)果與正常對(duì)照組比較,模型組細(xì)胞Vimentin和α-SMA表達(dá)增強(qiáng),E-cadherin表達(dá)減弱。羅勒多糖處理后Vimentin和α-SMA表達(dá)減弱,E-cadherin表達(dá)增強(qiáng)。TGF-β可提高細(xì)胞降解人工基底膜的能力,單位視野下侵襲至下室的細(xì)胞數(shù)模型組為69.0±4.3,與正常對(duì)照組(42.0±4.30)和羅勒多糖組(37.0±4.41)比較,差異有統(tǒng)計(jì)學(xué)意義,均P0.05。TGF-β增強(qiáng)了細(xì)胞的侵襲能力,羅勒多糖可以抑制TGF-β誘導(dǎo)的A549細(xì)胞侵襲能力。TGF-β使FN趨化的遷移細(xì)胞數(shù)目增多,單位視野下侵襲至下室的細(xì)胞數(shù)模型組為95.2±3.83,與正常對(duì)照組(69.2±5.11)和羅勒多糖組(68.2±6.18)比較,差異均有統(tǒng)計(jì)學(xué)意義,均P0.05。劃痕實(shí)驗(yàn)顯示,TGF-β增強(qiáng)了細(xì)胞的遷移能力,羅勒多糖可以抑制TGF-β誘導(dǎo)的A549細(xì)胞遷移能力。結(jié)論羅勒多糖能夠調(diào)節(jié)A549細(xì)胞上皮間質(zhì)轉(zhuǎn)化,這可能是其抑制A549細(xì)胞的侵襲轉(zhuǎn)移能力的重要機(jī)制。
[Abstract]:Objective basil polysaccharide has many biological activities, such as anti-tumor invasion and metastasis, chemotherapy synergy, hypoglycemia, blood lipid reduction and antithrombosis. The aim of this study was to investigate the inhibitory effect of basil polysaccharide on the invasiveness and motility of human lung cancer cell line A549 induced by transforming growth factor-尾 (transforming growth factor-beta,TGF- 尾 (transforming growth factor-beta,TGF- 尾), and to reveal the possible mechanism of basil polysaccharide inhibiting epithelial stroma transformation. Methods Lung cancer A549 cells were cultured in vitro and divided into three groups: (1) normal control group without any treatment; (2) model group, TGF- 尾 5 ng/mL; (3) basil polysaccharide group, TGF- 尾 5 ng/mL basil polysaccharide 200 渭 g / mL.. The effects of basil polysaccharide on the expression of E 鈮，

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