【摘要】：目的:檢測(cè)軟骨同源蛋白1(cartilage hemeprotein 1,CART1)在乳腺癌及癌旁組織中的表達(dá)水平,探討CART1的表達(dá)與乳腺癌病理特征的關(guān)系。運(yùn)用外源性RNA干擾技術(shù),敲低MDA-MB-231乳腺癌細(xì)胞中軟骨同源蛋白1(CART1)基因的表達(dá),研究CART1基因沉默后對(duì)MDA-MB-231細(xì)胞生物學(xué)行為的影響。方法:應(yīng)用免疫組織化學(xué)法檢測(cè)CART1在乳腺癌組織中的表達(dá),并統(tǒng)計(jì)分析CART1的表達(dá)量與乳腺癌患者的年齡腫瘤大小、病理分型、組織類型以及臨床分期的關(guān)系。采用人工合成的針對(duì)CART1基因的si RNA,運(yùn)用轉(zhuǎn)染試劑RNAi MAX轉(zhuǎn)染MDA-MB-231細(xì)胞。實(shí)時(shí)定量PCR檢測(cè)轉(zhuǎn)染后CART1 m RNA水平,Western blot法檢測(cè)轉(zhuǎn)染后CART1蛋白水平,Transwell TM侵襲實(shí)驗(yàn)檢測(cè)MDA-MB-231細(xì)胞侵襲CCK-8法檢測(cè)細(xì)胞增殖、流式細(xì)胞術(shù)檢測(cè)細(xì)胞周期的變化。結(jié)果:在62例乳腺癌組織標(biāo)本中,CART1在54例組織標(biāo)本高表達(dá)(87.1%);在8例癌旁組織中CART1不表達(dá)或者低表達(dá)。CART1-si RNA能有效沉默CART1在MDA-MB-231細(xì)胞中的表達(dá),CART1沉默后MDA-MB-231細(xì)胞侵襲和增殖能力明顯下降,S期細(xì)胞的比率增高而G2/M細(xì)胞比率減少。結(jié)論:CART1在乳腺癌組織中高表達(dá)。下調(diào)MDA-MB-231細(xì)胞CART1的表達(dá),可降低其侵襲和增殖能力、誘導(dǎo)S期阻滯。
[Abstract]:Aim: to detect the expression of cartilage homeoprotein 1 (cartilage hemeprotein 1 and Cart 1 in breast cancer and paracancerous tissues, and to explore the relationship between the expression of CART1 and pathological features of breast cancer. The expression of cartilage homeoprotein 1 (CART1) gene in MDA-MB-231 breast cancer cells was decreased by exogenous RNA interference technique, and the effect of CART1 gene silencing on the biological behavior of MDA-MB-231 cells was studied. Methods: the expression of CART1 in breast cancer tissues was detected by immunohistochemistry, and the expression of CART1 and the age of breast cancer patients were statistically analyzed. Relationship between tumor size, pathological classification, tissue type and clinical stage. MDA-MB-231 cells were transfected with synthetic si RNA, targeting CART1 gene by RNAi MAX. Real-time quantitative PCR was used to detect CART1 m RNA level, Western blot assay to detect CART1 protein level, Transwell TM invasion assay to detect MDA-MB-231 cell invasion CCK-8 assay to detect cell proliferation and flow cytometry to detect cell cycle changes. Results: in 62 cases of breast cancer, CART1 was highly expressed in 54 cases (87.1%). CART1 was not expressed or underexpressed in 8 cases of paracancerous tissues. CART1-si RNA could effectively silence the expression of CART1 in MDA-MB-231 cells, and the invasion and proliferation of MDA-MB-231 cells decreased significantly after CART1 silencing. The rate of S phase cells increased while that of G 2 鈮，

本文編號(hào)：2493524