【摘要】：目的1.通過(guò)檢測(cè)miR-143、miR-145在胰腺癌患者血清中的表達(dá)情況,比較miR-143、mi R-145表達(dá)水平在胰腺癌患者及健康志愿者血清中的差異。2.分析血清mi R-143、miR-145表達(dá)水平與胰腺癌患者臨床參數(shù)的相關(guān)性。方法收集30例胰腺癌患者和30例健康志愿者的血清,采用實(shí)時(shí)熒光定量PCR方法分別檢測(cè)胰腺癌患者血清、健康志愿者血清中miR-143、miR-145的表達(dá)水平。收集相關(guān)臨床資料,根據(jù)胰腺癌患者臨床病例參數(shù)(年齡、性別、腫瘤部位、腫瘤大小、TMN分期)對(duì)所收集的標(biāo)本進(jìn)行分組,采用SPSS19.0進(jìn)行統(tǒng)計(jì)學(xué)分析,探究胰腺癌血清中miR-143、mi R-145表達(dá)水平與其臨床參數(shù)的相關(guān)性。結(jié)果胰腺癌患者血清中mi R-143、miR-145表達(dá)水平顯著低于健康志愿者,差異有統(tǒng)計(jì)學(xué)意義(P0.05)�；颊哐逯衜iR-143、miR-145存在顯著正相關(guān)關(guān)系(P0.05)。logistics回歸分析顯示血清miR-143是胰腺癌發(fā)病的保護(hù)因素(P=0.036,OR=0.269)。胰腺癌患者血清miR-143、miR-145的表達(dá)水平在年齡、性別、腫瘤平均直徑、腫瘤部位、腫瘤TMN分期上的差異均無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論1.胰腺癌患者血清中mi R-143、mi R-145表達(dá)水平顯著低于健康志愿者,血清miR-143與mi R-145有顯著相關(guān)性,且血清miR-143是胰腺癌發(fā)病的保護(hù)因素。2.血清mi R-143、miR-145表達(dá)水平與胰腺癌腫瘤標(biāo)志物CA199及臨床預(yù)后指標(biāo)不存在相關(guān)性。
[Abstract]:Purpose 1. By detecting the expression of miR-143,miR-145 in serum of patients with pancreatic cancer, we compared the expression of miR-143,miR-145 in serum of patients with pancreatic cancer and healthy volunteers. To analyze the correlation between the expression of serum miR- 143, miR, 145 and clinical parameters in patients with pancreatic cancer. Methods the serum samples of 30 patients with pancreatic cancer and 30 healthy volunteers were collected. The expression of miR-143,miR-145 in the serum of pancreatic cancer patients and healthy volunteers was detected by real-time fluorescence quantitative PCR (real-time fluorescence quantitative PCR). According to the clinical parameters of pancreatic cancer patients (age, sex, tumor location, tumor size, TMN stage), the collected samples were divided into groups and analyzed statistically with SPSS19.0, and the clinical data were collected according to the clinical case parameters (age, sex, tumor location, tumor size, and tumor stage). Objective: to investigate the correlation between the expression of miR-143,mi-R-145 in serum of pancreatic cancer and its clinical parameters. Results the expression levels of miR- 143 and miRc 145 in serum of pancreatic cancer patients were significantly lower than those of healthy volunteers (P0.05). There was a significant positive correlation between miR-143,miR-145 in serum of patients (P0.05). Logistic regression analysis showed that serum miR-143 was a protective factor of pancreatic cancer (P = 0.036, OR = 0.269). There was no significant difference in serum miR-143,miR-145 expression in patients with pancreatic cancer in age, sex, average tumor diameter, tumor location and tumor TMN stage (P0.05). Conclusion 1. The expression of miR-143 and miR- 145 in serum of pancreatic cancer patients was significantly lower than that of healthy volunteers. Serum miR-143 was significantly correlated with miR- 145, and serum miR-143 was the protective factor of pancreatic cancer. There was no correlation between the expression of serum miR- 143 and miR 偽 145 and the tumor markers CA199 and clinical prognosis of pancreatic cancer.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R735.9

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 王志紅;周琳;王春峰;張連峰;;miR-143在胰腺癌組織中的表達(dá)及臨床意義[J];世界華人消化雜志;2016年10期

2 陳萬(wàn)青;鄭榮壽;張思維;曾紅梅;左婷婷;賈漫漫;夏昌發(fā);鄒小農(nóng);赫捷;;2012年中國(guó)惡性腫瘤發(fā)病和死亡分析[J];中國(guó)腫瘤;2016年01期

3 李衛(wèi)萍;李錦平;李元宏;任來(lái)峰;宋維芳;;miR-143通過(guò)靶向FASN抑制肝癌HepG2細(xì)胞脂質(zhì)形成[J];上海交通大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2015年03期

4 邢曉芳;李子禹;;miR-143和miR-145在胃癌中的表達(dá)及功能研究[J];中華胃腸外科雜志;2015年01期

5 王塬;逯忠堂;李堯清;紀(jì)棟;張鵬飛;劉青光;姚英民;;miR-143下調(diào)肝癌細(xì)胞TLR2的表達(dá)并抑制肝癌細(xì)胞增殖和侵襲[J];細(xì)胞與分子免疫學(xué)雜志;2014年10期

6 丁隆;楊宇;曲義坤;徐劍;劉偉新;張英海;;miR-143在HCC組織中的表達(dá)及其臨床意義[J];中國(guó)肝臟病雜志(電子版);2014年03期

7 謝云;田洲;渠亞超;鮑旭麗;張蕾;閭軍;;血清microRNA-143在乙型肝炎病毒相關(guān)性肝病中的表達(dá)和臨床意義[J];中華臨床醫(yī)師雜志(電子版);2013年24期

8 Xiao-Li Wu;Bin Cheng;Pei-Yuan Li;Huan-Jun Huang;Qiu Zhao;Zi-Li Dan;Dean Tian;Peng Zhang;;MicroRNA-143 suppresses gastric cancer cell growth and induces apoptosis by targeting COX-2[J];World Journal of Gastroenterology;2013年43期

9 程文捷;唐健;黃鳳婷;莊燕妍;陳文博;張世能;;微小RNA-143對(duì)人胰腺癌PANC-1細(xì)胞遷移的抑制作用及其機(jī)制的探討[J];腫瘤;2012年11期

10 由法平;宋孟，

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