【摘要】：目的:探討ZKSCAN3(zinc finger with KRAB and SCAN domains 3)在乳腺癌組織中的表達(dá)及其臨床意義。方法 :應(yīng)用免疫組織化學(xué)法檢測120例乳腺癌組織及60例癌旁組織中ZKSCAN3蛋白的表達(dá),并分析其與患者臨床病理參數(shù)和總生存率之間的關(guān)系。進一步應(yīng)用實時熒光定量PCR法和蛋白質(zhì)印跡法檢測乳腺癌組織及其相應(yīng)癌旁組織中ZKSCAN3 m RNA和蛋白的表達(dá)水平。結(jié)果:乳腺癌組織中ZKSCAN3蛋白的陽性表達(dá)率[74%(89/120)]高于相應(yīng)的癌旁組織[37%(22/60)](P0.001)。ZKSCAN3蛋白的表達(dá)與乳腺癌患者腫塊大小、淋巴結(jié)轉(zhuǎn)移、分化程度和腫瘤分期相關(guān)(P值均0.05),ZKSCAN3陽性表達(dá)患者的總生存率明顯低于陰性患者(P=0.040)。乳腺癌組織中ZKSCAN3 m RNA和蛋白的表達(dá)水平均高于癌旁組織(P值均0.01)。結(jié)論 :ZKSCAN3與乳腺癌的發(fā)生、進展及轉(zhuǎn)移有關(guān),可能是乳腺癌的促癌基因。
[Abstract]:Objective: to investigate the expression of ZKSCAN3 (zinc finger with KRAB and SCAN domains-3 in breast cancer and its clinical significance. Methods: immunohistochemical method was used to detect the expression of ZKSCAN3 protein in 120 cases of breast cancer and 60 cases of paracancerous tissue, and the relationship between the expression and clinicopathological parameters and overall survival rate was analyzed. Real-time fluorescence quantitative PCR and Western blot were used to detect the expression of ZKSCAN3 m RNA and protein in breast cancer tissues and their corresponding paracancerous tissues. Results: the positive expression rate of ZKSCAN3 protein in breast cancer [74% (89 / 120)] was higher than that in paracancerous tissue [37% (22 / 60)] (P0.001). The expression of ZKSCAN3 protein was associated with tumor size and lymph node metastasis in patients with breast cancer. The overall survival rate of patients with positive expression of ZKSCAN3 was significantly lower than that of patients with negative expression (P < 0. 040). The expression level of ZKSCAN3 m RNA and protein in breast cancer tissues was higher than that in adjacent tissues (P < 0 01). Conclusion: ZKSCAN3 is associated with the occurrence, progression and metastasis of breast cancer, and may be a prooncogene of breast cancer.
【作者單位】： 鄭州大學(xué)第一附屬醫(yī)院腫瘤科;鄭州大學(xué)第一附屬醫(yī)院乳腺外科;
【分類號】：R737.9
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本文編號：2442911