CFTR與E-cadherin蛋白在結(jié)直腸癌中的表達(dá)及臨床意義
發(fā)布時(shí)間：2019-02-16 00:13
【摘要】：結(jié)直腸癌是一種常見的消化道惡性腫瘤疾病,在我們國家,結(jié)直腸癌的發(fā)病率和死亡率均高于世界平均水平。由于臨床常常確診較晚,治療費(fèi)用較高,造成了嚴(yán)重的家庭經(jīng)濟(jì)負(fù)擔(dān)和社會(huì)負(fù)擔(dān)。結(jié)直腸癌的發(fā)病機(jī)制和臨床治療是目前研究的熱點(diǎn)和難點(diǎn)。本實(shí)驗(yàn)通過研究囊性纖維化跨膜傳導(dǎo)調(diào)節(jié)因子(cystic fibrosis transmembrane conductance regulator CFTR)與E-cadherin蛋白在結(jié)直腸癌組織和正常結(jié)直腸黏膜組織中的表達(dá)情況,探討兩者的表達(dá)與臨床病理特征之間的關(guān)系。同時(shí)探討CFTR與E-cadherin蛋白在結(jié)直腸癌組織中的表達(dá)之間的相互關(guān)系。為進(jìn)一步研究CFTR與E-cadherin蛋白在結(jié)直腸癌疾病進(jìn)程中所起的作用機(jī)制以及腫瘤的預(yù)防和治療提供理論依據(jù)支持。本實(shí)驗(yàn)收集解放軍第309醫(yī)院普通外科2012.02-2013.08手術(shù)切除的結(jié)直腸癌組織標(biāo)本62例作為實(shí)驗(yàn)組(A組),收集相應(yīng)腫瘤患者距離腫瘤組織大于5cm處正常結(jié)直腸黏膜組織6 0例作為對(duì)照組(B組)。其中A組男38例,女24例,年齡37~83之間,平均年齡55.4歲;其中高分化腺癌12例,中低分化腺癌27例,低分化腺癌23例;TNM分期:I期11例,II期26例,III期25例;其中有淋巴結(jié)轉(zhuǎn)移39例,無淋巴結(jié)轉(zhuǎn)移23例;有漿膜浸潤的有35例,無漿膜浸潤的27例。采用免疫組織化學(xué)方法(En Vision法)觀察CFTR與E-cadherin蛋白在結(jié)直腸癌組織和正常結(jié)直腸黏膜組織中的表達(dá),結(jié)果表明,CFTR及E-cadherin蛋白在結(jié)直腸癌中的表達(dá)和在正常黏膜組織相比顯著降低,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。CFTR及E-cadherin蛋白在結(jié)直腸癌中的表達(dá)具有一定的相關(guān)性,兩者呈正相關(guān)(R=0.403,P0.05)。CFTR與E-cadherin蛋白表達(dá)與腫瘤的TNM分期、分化程度、漿膜浸潤、淋巴結(jié)轉(zhuǎn)移有關(guān),而與患者性別、年齡及腫瘤大小無關(guān)。以上結(jié)果提示,CFTR及E-cadherin蛋白在結(jié)直腸癌發(fā)生、局部浸潤和遠(yuǎn)處轉(zhuǎn)移有關(guān),兩者共同參與了結(jié)直腸癌的發(fā)病過程。CFTR及E-cadherin蛋白的聯(lián)合檢測對(duì)于結(jié)直腸的早期診斷,預(yù)測腫瘤的侵襲和轉(zhuǎn)移有重要意義,CFTR可能為治療結(jié)直腸癌提供一個(gè)新的基因靶點(diǎn)和策略。
[Abstract]:Colorectal cancer is a common malignant disease of digestive tract. In our country, the morbidity and mortality of colorectal cancer are higher than the world average. Because of the late diagnosis and the high cost of treatment, the family economic burden and social burden are serious. The pathogenesis and clinical treatment of colorectal cancer are hot and difficult. In this study, we studied the expression of (cystic fibrosis transmembrane conductance regulator CFTR) and E-cadherin protein in colorectal carcinoma and normal colorectal mucosa. To explore the relationship between the expression and clinicopathological features of the two. At the same time, the relationship between the expression of CFTR and E-cadherin protein in colorectal cancer was studied. To further study the role of CFTR and E-cadherin protein in the progression of colorectal cancer, and provide theoretical basis for the prevention and treatment of tumor. In this experiment, 62 specimens of colorectal cancer were collected from the general surgery department of the 309 PLA Hospital as the experimental group (group A). 60 cases of normal colorectal mucosa from tumor tissue larger than 5cm were collected as control group (group B). In group A, there were 38 males and 24 females, aged between 3783 and 55.4 years, including 12 cases of well-differentiated adenocarcinoma, 27 cases of poorly differentiated adenocarcinoma, 23 cases of poorly differentiated adenocarcinoma, 11 cases of stage I, 26 cases of II and 25 cases of III. There were 39 cases of lymph node metastasis, 23 cases of no lymph node metastasis, 35 cases of serosal infiltration and 27 cases of no serous infiltration. The expression of CFTR and E-cadherin protein in colorectal carcinoma and normal colorectal mucosa was observed by immunohistochemical method (En Vision). The expression of CFTR and E-cadherin in colorectal cancer was significantly lower than that in normal mucosa (P0.05). The expression of). CFTR and E-cadherin was positively correlated with the TNM stage, differentiation, serosal infiltration and lymph node metastasis, but not with the sex, age and tumor size of the tumor. These results suggest that CFTR and E-cadherin proteins are involved in the pathogenesis, local invasion and distant metastasis of colorectal cancer, and both are involved in the pathogenesis of colorectal cancer. The combined detection of CFTR and E-cadherin proteins is associated with the early diagnosis of colorectal cancer. It is important to predict the invasion and metastasis of tumors. CFTR may provide a new gene target and strategy for the treatment of colorectal cancer.
【學(xué)位授予單位】：河北北方學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R735.34
本文編號(hào)：2423839
[Abstract]:Colorectal cancer is a common malignant disease of digestive tract. In our country, the morbidity and mortality of colorectal cancer are higher than the world average. Because of the late diagnosis and the high cost of treatment, the family economic burden and social burden are serious. The pathogenesis and clinical treatment of colorectal cancer are hot and difficult. In this study, we studied the expression of (cystic fibrosis transmembrane conductance regulator CFTR) and E-cadherin protein in colorectal carcinoma and normal colorectal mucosa. To explore the relationship between the expression and clinicopathological features of the two. At the same time, the relationship between the expression of CFTR and E-cadherin protein in colorectal cancer was studied. To further study the role of CFTR and E-cadherin protein in the progression of colorectal cancer, and provide theoretical basis for the prevention and treatment of tumor. In this experiment, 62 specimens of colorectal cancer were collected from the general surgery department of the 309 PLA Hospital as the experimental group (group A). 60 cases of normal colorectal mucosa from tumor tissue larger than 5cm were collected as control group (group B). In group A, there were 38 males and 24 females, aged between 3783 and 55.4 years, including 12 cases of well-differentiated adenocarcinoma, 27 cases of poorly differentiated adenocarcinoma, 23 cases of poorly differentiated adenocarcinoma, 11 cases of stage I, 26 cases of II and 25 cases of III. There were 39 cases of lymph node metastasis, 23 cases of no lymph node metastasis, 35 cases of serosal infiltration and 27 cases of no serous infiltration. The expression of CFTR and E-cadherin protein in colorectal carcinoma and normal colorectal mucosa was observed by immunohistochemical method (En Vision). The expression of CFTR and E-cadherin in colorectal cancer was significantly lower than that in normal mucosa (P0.05). The expression of). CFTR and E-cadherin was positively correlated with the TNM stage, differentiation, serosal infiltration and lymph node metastasis, but not with the sex, age and tumor size of the tumor. These results suggest that CFTR and E-cadherin proteins are involved in the pathogenesis, local invasion and distant metastasis of colorectal cancer, and both are involved in the pathogenesis of colorectal cancer. The combined detection of CFTR and E-cadherin proteins is associated with the early diagnosis of colorectal cancer. It is important to predict the invasion and metastasis of tumors. CFTR may provide a new gene target and strategy for the treatment of colorectal cancer.
【學(xué)位授予單位】：河北北方學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R735.34
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 李亞秋;姜文華;范軍達(dá);郝利銘;;CFTR在肺鱗癌中的表達(dá)及其與腫瘤凋亡關(guān)系的研究[J];解剖科學(xué)進(jìn)展;2013年04期
，本文編號(hào)：2423839
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