【摘要】：目的:探討miR-503是否能通過(guò)調(diào)控bcl-2的表達(dá)增強(qiáng)BEL-7402細(xì)胞對(duì)順鉑的敏感性。方法:采用實(shí)時(shí)熒光定量PCR檢測(cè)肝癌細(xì)胞中miR-503和bcl-2 mRNA的表達(dá)水平;Western印跡觀察肝癌細(xì)胞中Bcl-2蛋白的表達(dá)水平;脂質(zhì)體轉(zhuǎn)染法將miR-503模擬物瞬時(shí)轉(zhuǎn)染至BEL-7402細(xì)胞;生物信息學(xué)軟件預(yù)測(cè)miR-503潛在靶基因;雙熒光素酶活性驗(yàn)證miR-503潛在的靶位點(diǎn);MTT法檢測(cè)細(xì)胞藥物敏感性的改變。結(jié)果:相比于HL-7702細(xì)胞,miR-503在BEL-7402細(xì)胞中表達(dá)水平下調(diào),Bcl-2蛋白的表達(dá)水平上調(diào);miR-503能夠與bcl-2靶向結(jié)合,下調(diào)bcl-2的表達(dá);miR-503轉(zhuǎn)染組的細(xì)胞活力較miRNA陰性對(duì)照組顯著降低。結(jié)論:MiR-503可能通過(guò)靶向bcl-2增強(qiáng)BEL-7402細(xì)胞對(duì)順鉑的藥物敏感性,抑制肝癌細(xì)胞增殖。
[Abstract]:Aim: to investigate whether miR-503 can enhance the sensitivity of BEL-7402 cells to cisplatin by regulating the expression of bcl-2. Methods: the expression of miR-503 and bcl-2 mRNA in hepatoma cells was detected by real-time fluorescence quantitative PCR, and the expression of Bcl-2 protein was detected by Western blot. MiR-503 mimics were transiently transfected into BEL-7402 cells by liposome transfection; bioinformatics software was used to predict the potential target genes of miR-503; double luciferase activity was used to verify the potential target sites of miR-503; and the changes in drug sensitivity of cells were detected by MTT assay. Results: compared with HL-7702 cells, the expression of miR-503 in BEL-7402 cells was down-regulated, the expression of Bcl-2 protein was up-regulated, and miR-503 could target bcl-2 and down-regulate the expression of bcl-2. The cell viability of miR-503 transfection group was significantly lower than that of miRNA negative control group. Conclusion: MiR-503 may enhance the chemosensitivity of BEL-7402 cells to cisplatin by targeting bcl-2 and inhibit the proliferation of HCC cells.
【作者單位】： 南華大學(xué)生物研究所;南華大學(xué)湖南省分子靶標(biāo)新藥研究協(xié)同創(chuàng)新中心;南華大學(xué)藥物藥理研究所;南華大學(xué)腫瘤研究所;
【基金】：國(guó)家自然科學(xué)基金(81372579) 湖南省教育廳科學(xué)研究項(xiàng)目(17C1402,14C0998) 湖南省研究生創(chuàng)新項(xiàng)目(CX2015B386)~~
【分類號(hào)】：R735.7

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