【摘要】：目的:研究體外共培養(yǎng)情況下人胚胎干細(xì)胞H9對(duì)肝癌HepG2細(xì)胞的抑制作用。方法:建立人胚胎干細(xì)胞(H9)與肝癌HepG2細(xì)胞共培養(yǎng)體系,顯微鏡下觀察胚胎干細(xì)胞對(duì)腫瘤細(xì)胞生物學(xué)行為的影響,流式細(xì)胞儀檢測(cè)H9細(xì)胞對(duì)腫瘤細(xì)胞的凋亡與周期的影響,Transwell小室法檢測(cè)H9細(xì)胞對(duì)HepG2細(xì)胞侵襲和遷移的影響,基因芯片分析共培養(yǎng)后HepG2細(xì)胞全基因組的表達(dá)譜變化。結(jié)果:結(jié)果發(fā)現(xiàn)共培養(yǎng)過(guò)程中,肝癌HepG2細(xì)胞生長(zhǎng)受到抑制,隨共培養(yǎng)時(shí)間延長(zhǎng),細(xì)胞數(shù)量逐漸減少,出現(xiàn)老化或凋亡跡象;流式細(xì)胞術(shù)檢測(cè)發(fā)現(xiàn)HepG2細(xì)胞凋亡率顯著增加,細(xì)胞周期被阻滯于G0/G1期;Transwell實(shí)驗(yàn)發(fā)現(xiàn)HepG2細(xì)胞侵襲、遷移力均降低;基因芯片結(jié)果發(fā)現(xiàn)HepG2細(xì)胞全基因組表達(dá)譜發(fā)生了顯著變化,差異基因涉及多條信號(hào)通路。結(jié)論:人胚胎干細(xì)胞H9體外對(duì)人肝癌細(xì)胞HepG2有一定程度的抑制作用。
[Abstract]:Aim: to study the inhibitory effect of human embryonic stem cell H 9 on HepG2 cells in vitro. Methods: the co-culture system of human embryonic stem cells (H9) and hepatocellular carcinoma (HepG2) cells was established. The effects of embryonic stem cells on the biological behavior of tumor cells were observed under microscope, and the effects of H9 cells on apoptosis and cell cycle were detected by flow cytometry. The effect of H9 cells on the invasion and migration of HepG2 cells was detected by Transwell chamber assay. The gene microarray was used to analyze the expression profile of the whole genome of HepG2 cells after co-culture. Results: the results showed that the growth of hepatoma HepG2 cells was inhibited during co-culture. With the prolongation of co-culture time, the number of hepatoma cells decreased gradually, and the cells showed signs of aging or apoptosis. Flow cytometry showed that the apoptosis rate of HepG2 cells was significantly increased, cell cycle was blocked in G0/G1 phase, and HepG2 cell invasion and migration decreased by Transwell assay. The results of gene chip showed that the whole genome expression profile of HepG2 cells changed significantly, and the differentially expressed genes were involved in many signal pathways. Conclusion: human embryonic stem cell H 9 has a certain inhibitory effect on human hepatoma cell HepG2 in vitro.
【作者單位】： 重慶醫(yī)科大學(xué)分子醫(yī)學(xué)與腫瘤研究中心;重慶醫(yī)科大學(xué)分子醫(yī)學(xué)與腫瘤研究中心重慶市生物化學(xué)與分子藥理學(xué)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(81071770,81201679)
【分類號(hào)】：R73-36

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