【摘要】：目的觀察結(jié)膜黏膜相關(guān)淋巴組織(MALT)淋巴瘤中microRNA-150(miR-150)的表達(dá),探討miR-150在結(jié)膜MALT淋巴瘤中影響腫瘤細(xì)胞增殖、遷移與侵襲的機(jī)制。方法使用qPCR法檢測第二軍醫(yī)大學(xué)長征醫(yī)院收治的3例結(jié)膜MALT淋巴瘤患者腫瘤組織及瘤旁組織中miR-150及其可能的下游靶分子Cbl-b的表達(dá)。將miR-150抑制物和陰性對(duì)照轉(zhuǎn)染人多發(fā)性骨髓瘤細(xì)胞株RPMI 8226,采用CCK-8法和流式細(xì)胞術(shù)研究miR-150對(duì)RPMI 8226細(xì)胞增殖和凋亡的影響,通過Transwell實(shí)驗(yàn)研究miR-150對(duì)RPMI 8226細(xì)胞遷移和侵襲的影響,用蛋白質(zhì)印跡法檢測miR-150對(duì)RPMI 8226細(xì)胞中Cbl-b表達(dá)的調(diào)控。結(jié)果與瘤旁組織相比,結(jié)膜MALT淋巴瘤組織中miR-150表達(dá)上調(diào)(P0.05,P0.01);抑制miR-150后,RPMI 8226細(xì)胞的增殖受到抑制,細(xì)胞凋亡明顯增加,遷移和侵襲能力降低,與陰性對(duì)照組相比差異有統(tǒng)計(jì)學(xué)意義(P0.05,P0.01)。在結(jié)膜MALT淋巴瘤組織中,miR-150下游靶基因Cbl-b表達(dá)下調(diào)(P0.01);抑制miR-150后,RPMI 8226細(xì)胞內(nèi)Cbl-b蛋白的表達(dá)上調(diào)(P0.01)。結(jié)論 MiR-150對(duì)淋巴瘤細(xì)胞的增殖、遷移和侵襲有促進(jìn)作用,其表達(dá)上調(diào)參與了MALT淋巴瘤的發(fā)生。其機(jī)制可能與miR-150對(duì)下游分子Cbl-b的抑制性調(diào)控有關(guān)。
[Abstract]:Objective to observe the expression of microRNA-150 (miR-150) in conjunctival mucosa-associated lymphoid tissue (MALT) lymphoma and to explore the mechanism of miR-150 affecting the proliferation, migration and invasion of tumor cells in conjunctival MALT lymphoma. Methods qPCR method was used to detect the expression of miR-150 and its possible downstream target Cbl-b in tumor tissues and adjacent tissues of 3 patients with conjunctival MALT lymphoma treated in Changzheng Hospital of second military Medical University. Human multiple myeloma cell line RPMI 8226 was transfected with miR-150 inhibitor and negative control. The effect of miR-150 on proliferation and apoptosis of RPMI 8226 cells was studied by CCK-8 assay and flow cytometry. The effect of miR-150 on the migration and invasion of RPMI 8226 cells was studied by Transwell assay. The regulation of Cbl-b expression in RPMI 8226 cells by miR-150 was detected by Western blotting. Results the expression of miR-150 was up-regulated in conjunctival MALT lymphoma (P0.05, P0.01). After inhibiting miR-150, the proliferation of RPMI 8226 cells was inhibited, the apoptosis of RPMI 8226 cells was significantly increased, the migration and invasion ability was decreased, compared with the negative control group, the difference was statistically significant (P0.05, P0.01). In conjunctival MALT lymphoma, the Cbl-b expression of miR-150 downstream target gene was down-regulated (P0.01), and the expression of Cbl-b protein was up-regulated in RPMI 8226 cells after miR-150 inhibition (P0.01). Conclusion MiR-150 can promote the proliferation, migration and invasion of Lymphoma cells, and its up-regulation may be involved in the pathogenesis of MALT lymphoma. The mechanism may be related to the inhibitory regulation of Cbl-b by miR-150.
【作者單位】： 第二軍醫(yī)大學(xué)長征醫(yī)院眼科;
【基金】：上海市自然科學(xué)基金(14ZR1414000) 上海市衛(wèi)生和計(jì)劃生育委員會(huì)科研課題面上項(xiàng)目(201445)~~
【分類號(hào)】：R739.7

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