【摘要】：目的探討HERC4對(duì)宮頸癌細(xì)胞生物學(xué)功能的影響及其分子機(jī)制。方法設(shè)計(jì)特異HERC4 si RNA干擾序列,Lipofectamine2000法轉(zhuǎn)染Hela細(xì)胞,Western blotting檢測轉(zhuǎn)染特異si RNA后Hela細(xì)胞HERC4蛋白的表達(dá)水平;CCK-8法檢測轉(zhuǎn)染后Hela細(xì)胞的增殖能力;AnnexinⅤ-FITC/PI雙染法檢測轉(zhuǎn)染后Hela細(xì)胞凋亡率的變化;劃痕實(shí)驗(yàn)檢測轉(zhuǎn)染后Hela細(xì)胞遷移能力。Western blotting檢測轉(zhuǎn)染后Hela細(xì)胞Cyclin D1、Bcl-2表達(dá)變化。結(jié)果轉(zhuǎn)染特異si RNA后Hela細(xì)胞HERC4蛋白表達(dá)量明顯下降,差異有統(tǒng)計(jì)學(xué)意義(P0.01)。與對(duì)照組相比,干擾組Hela細(xì)胞生長速度明顯減慢,細(xì)胞凋亡率增加,遷移能力明顯下降,差異有統(tǒng)計(jì)學(xué)意義(P0.01)。轉(zhuǎn)染后Cyclin D1和Bcl-2在蛋白水平的表達(dá)顯著下調(diào)(P0.01)。結(jié)論 si RNA可有效降低宮頸癌Hela細(xì)胞中HERC4的表達(dá),并通過抑制Cyclin D1表達(dá)抑制宮頸癌Hela細(xì)胞的增殖和遷移能力,抑制Bcl-2表達(dá)增加細(xì)胞凋亡能力。
[Abstract]:Objective to investigate the effect of HERC4 on the biological function of cervical cancer cells and its molecular mechanism. Methods the specific HERC4 si RNA interference sequence was designed and the expression level of HERC4 protein in Hela cells after transfection of specific si RNA was detected by, Western blotting with Lipofectamine2000 method, and the proliferation ability of Hela cells was detected by CCK-8 method. The apoptosis rate of Hela cells after transfection was detected by Annexin 鈪，

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