【摘要】：不可逆電穿孔腫瘤消融的有效性與安全性已經(jīng)過多年的臨床應(yīng)用而得到驗證,新近發(fā)展起來的高頻不可逆電穿孔腫瘤消融新方法兼具傳統(tǒng)不可逆電穿孔的優(yōu)勢和緩解肌肉收縮的長處,然而目前對其作用機理還未深入研究。為此,從阻抗譜分析角度分析了高頻不可逆電穿孔與傳統(tǒng)不可逆電穿孔組織消融的差異性,研究了相同劑量下高頻不可逆電穿孔與傳統(tǒng)不可逆電穿孔消融新西蘭大白兔肝臟組織引起的組織阻抗譜變化,并通過等效電路的擬合提取了阻抗譜變化中的信息。研究結(jié)果表明:脈沖處理后組織阻抗譜顯著變化,阻抗模在中低頻段顯著減小,阻抗角在中高頻段從負值向零值方向變化;通過等效電路擬合發(fā)現(xiàn),處理后顯著變化的是細胞外液等效電阻,由于細胞膜穿孔而增加了新的與細胞外液等效電阻并聯(lián)的電流通路;脈沖劑量相同條件下,傳統(tǒng)不可逆電穿孔脈沖作用引起的細胞外液等效電阻變化最大,高頻不可逆電穿孔作用下,脈沖寬度越寬,細胞外液等效電阻變化就越顯著。因此,組織阻抗譜變化及由它得到的等效電路元器件值變化,可以有效地反映電穿孔程度,有望成為臨床應(yīng)用中檢測不可逆電穿孔治療效果的有效方式。
[Abstract]:The efficacy and safety of irreversible electroporation ablation have been demonstrated in clinical practice for many years. Recently, the new ablation method of high frequency irreversible electroporation (HF irreversibly electroporation) has both the advantages of traditional irreversible electroporation and the advantages of relieving muscle contraction. However, the mechanism of its action has not been thoroughly studied. Therefore, the difference of tissue ablation between high frequency irreversible electroporation and traditional irreversible electroporation was analyzed from the point of view of impedance spectrum analysis. The changes of tissue impedance spectrum induced by high frequency irreversible electroporation and traditional irreversible electroporation ablation in New Zealand white rabbit liver were studied at the same dose. The information of impedance spectrum change was extracted by fitting the equivalent circuit. The results show that after pulse treatment, the tissue impedance spectrum changes significantly, the impedance mode decreases significantly in the middle and low frequency band, and the impedance angle changes from negative to zero in the medium and high frequency range. Through the equivalent circuit fitting, it was found that the extracellular fluid equivalent resistance changed significantly after treatment, and a new current path parallel to the extracellular fluid equivalent resistance was added due to the membrane perforation. Under the same pulse dose, the effect of the traditional irreversible electroporation pulse on the extracellular fluid equivalent resistance is the largest. The wider the pulse width is, the more obvious the change of the extracellular fluid equivalent resistance will be under the action of high frequency irreversible electroporation. Therefore, the change of tissue impedance spectrum and the changes of equivalent circuit components can effectively reflect the degree of electroporation, which is expected to be an effective way to detect the effect of irreversible electroporation in clinical application.
【作者單位】： 重慶大學電氣工程學院輸配電裝備及系統(tǒng)安全與新技術(shù)國家重點實驗室;Bioelectromechanical
【基金】：國家創(chuàng)新研究群體基金(51321063) 重慶市杰出青年基金(cstc2014jcyjjq90001) 中央高�；究蒲袠I(yè)務(wù)費資助(106112017CDJQJ158835)~~
【分類號】：R73-36
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本文編號：2391038