【摘要】：目的 本研究旨在探究核因子IA(nuclear factor IA,NFIA)在膠質(zhì)瘤發(fā)生發(fā)展中的作用機(jī)制,并觀(guān)察NFIA和ezrin在膠質(zhì)瘤中表達(dá)相關(guān)性,并進(jìn)一步探討NFIA促進(jìn)膠質(zhì)瘤細(xì)胞侵襲轉(zhuǎn)移的分子機(jī)制。方法使用siRNA干擾技術(shù)處理膠質(zhì)瘤U251細(xì)胞,采用實(shí)時(shí)熒光定量PCR(realtime-PCR)和Western blot檢測(cè)NFIA表達(dá)水平的變化,采用劃痕實(shí)驗(yàn)及transwell小室侵襲實(shí)驗(yàn)檢測(cè)敲低NFIA蛋白表達(dá)對(duì)膠質(zhì)瘤細(xì)胞侵襲、遷移能力的變化。結(jié)果 下調(diào)膠質(zhì)瘤細(xì)胞株中NFIA后,NFIA干擾組細(xì)胞生長(zhǎng)明顯減慢,與陰性對(duì)照組與空白對(duì)照組比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。劃痕實(shí)驗(yàn)顯示NFIA干擾組細(xì)胞遷移距離(57.9±6.9)μm明顯低于陰性對(duì)照組(125.4±10.7)μm和空白對(duì)照組(111.5±5.9)μm,差異有統(tǒng)計(jì)學(xué)意義(P0.05),Transwell實(shí)驗(yàn)顯示RNA干擾組穿膜細(xì)胞數(shù)(33.3±3.2)低于陰性對(duì)照組和空白對(duì)照組(80.3±4.5、87.7±5.0),差異有統(tǒng)計(jì)學(xué)意義(P0.05)。Western blot結(jié)果顯示,NFIA干擾組Ezrin的蛋白相對(duì)表達(dá)顯著低于陰性對(duì)照組與空白對(duì)照組,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。討論 在膠質(zhì)瘤組織中NFIA表達(dá)明顯高于正常腦組織,下調(diào)NFIA蛋白表達(dá)可抑制膠質(zhì)瘤細(xì)胞侵襲和遷移能力。
[Abstract]:Objective to investigate the role of nuclear factor IA (nuclear factor IA,NFIA in the pathogenesis of glioma, and to investigate the correlation between NFIA and ezrin expression in gliomas, and to explore the molecular mechanism of NFIA promoting the invasion and metastasis of glioma cells. Methods siRNA interference technique was used to treat U251 glioma cells and real-time quantitative PCR (realtime-PCR) and Western blot were used to detect the expression of NFIA. Scratch test and transwell chamber invasion assay were used to detect the changes of the invasion and migration ability of glioma cells induced by low expression of NFIA protein. Results after down-regulation of NFIA in glioma cell line, the cell growth of NFIA interference group was significantly slower than that of negative control group and blank control group (P0.05). The cell migration distance of NFIA interference group (57.9 鹵6.9) 渭 m was significantly lower than that of negative control group (125.4 鹵10.7) 渭 m and blank control group (111.5 鹵5.9) 渭 m (P0.05). Transwell test showed that the number of perforating cells in RNA interference group (33.3 鹵3.2) was significantly lower than that in negative control group and blank control group (80.3 鹵4.5 鹵87.7 鹵5.0), and the difference was statistically significant (P0.05). Western blot). The relative expression of Ezrin protein in NFIA interference group was significantly lower than that in negative control group and blank control group (P0.05). The expression of NFIA in glioma tissue was significantly higher than that in normal brain tissue, and down-regulation of NFIA protein expression could inhibit the invasion and migration of glioma cells.
【學(xué)位授予單位】：皖南醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R739.41

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