【摘要】：目的 本研究旨在探究核因子IA(nuclear factor IA,NFIA)在膠質瘤發(fā)生發(fā)展中的作用機制,并觀察NFIA和ezrin在膠質瘤中表達相關性,并進一步探討NFIA促進膠質瘤細胞侵襲轉移的分子機制。方法使用siRNA干擾技術處理膠質瘤U251細胞,采用實時熒光定量PCR(realtime-PCR)和Western blot檢測NFIA表達水平的變化,采用劃痕實驗及transwell小室侵襲實驗檢測敲低NFIA蛋白表達對膠質瘤細胞侵襲、遷移能力的變化。結果 下調膠質瘤細胞株中NFIA后,NFIA干擾組細胞生長明顯減慢,與陰性對照組與空白對照組比較,差異均有統(tǒng)計學意義(P0.05)。劃痕實驗顯示NFIA干擾組細胞遷移距離(57.9±6.9)μm明顯低于陰性對照組(125.4±10.7)μm和空白對照組(111.5±5.9)μm,差異有統(tǒng)計學意義(P0.05),Transwell實驗顯示RNA干擾組穿膜細胞數(shù)(33.3±3.2)低于陰性對照組和空白對照組(80.3±4.5、87.7±5.0),差異有統(tǒng)計學意義(P0.05)。Western blot結果顯示,NFIA干擾組Ezrin的蛋白相對表達顯著低于陰性對照組與空白對照組,差異均有統(tǒng)計學意義(P0.05)。討論 在膠質瘤組織中NFIA表達明顯高于正常腦組織,下調NFIA蛋白表達可抑制膠質瘤細胞侵襲和遷移能力。
[Abstract]:Objective to investigate the role of nuclear factor IA (nuclear factor IA,NFIA in the pathogenesis of glioma, and to investigate the correlation between NFIA and ezrin expression in gliomas, and to explore the molecular mechanism of NFIA promoting the invasion and metastasis of glioma cells. Methods siRNA interference technique was used to treat U251 glioma cells and real-time quantitative PCR (realtime-PCR) and Western blot were used to detect the expression of NFIA. Scratch test and transwell chamber invasion assay were used to detect the changes of the invasion and migration ability of glioma cells induced by low expression of NFIA protein. Results after down-regulation of NFIA in glioma cell line, the cell growth of NFIA interference group was significantly slower than that of negative control group and blank control group (P0.05). The cell migration distance of NFIA interference group (57.9 鹵6.9) 渭 m was significantly lower than that of negative control group (125.4 鹵10.7) 渭 m and blank control group (111.5 鹵5.9) 渭 m (P0.05). Transwell test showed that the number of perforating cells in RNA interference group (33.3 鹵3.2) was significantly lower than that in negative control group and blank control group (80.3 鹵4.5 鹵87.7 鹵5.0), and the difference was statistically significant (P0.05). Western blot). The relative expression of Ezrin protein in NFIA interference group was significantly lower than that in negative control group and blank control group (P0.05). The expression of NFIA in glioma tissue was significantly higher than that in normal brain tissue, and down-regulation of NFIA protein expression could inhibit the invasion and migration of glioma cells.
【學位授予單位】：皖南醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R739.41

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