【摘要】：背景與目的OLC1(overexpressed in lung cancer 1)是一個新型肺癌相關(guān)基因,前期的研究發(fā)現(xiàn),在肺鱗癌、腺癌、小細(xì)胞癌組織中均有較高表達(dá),OLC1蛋白在肺癌患者外周血漿中的表達(dá)顯著高于正常人,并可作為肺癌的輔助診斷的候選標(biāo)志物。OLC1在食管鱗癌、卵巢癌、乳腺癌、結(jié)直腸癌組織中也有較高表達(dá),并與不良預(yù)后相關(guān),但OLC1與肺癌患者預(yù)后的關(guān)系一直缺乏相關(guān)研究。本研究通過檢測OLC1在非小細(xì)胞肺癌(NSCLC)患者癌組織中的蛋白表達(dá)情況,結(jié)合預(yù)后資料,分析OLC1的過表達(dá)與肺癌患者預(yù)后的關(guān)系。方法分別對包含75例鱗癌和90例腺癌的癌組織和癌旁組織的組織芯片以及33例鱗癌組織切片進(jìn)行免疫組化染色,檢測OLC1蛋白的表達(dá)水平,分析其在組織中的表達(dá)與臨床特征及預(yù)后的關(guān)系。結(jié)果OLC1在腺癌患者癌組織中的過表達(dá)率(87.5%)明顯高于鱗癌(55.3%,P0.001)。OLC1在腺癌患者癌組織中的過表達(dá)與預(yù)后無顯著相關(guān)性,在鱗癌組織中的過表達(dá)與不良預(yù)后相關(guān),單因素Kaplan-Meier生存分析提示OLC1過表達(dá)的患者具有更差的預(yù)后(P=0.042),多因素Cox回歸分析顯示兩組之間的生存差異無統(tǒng)計學(xué)意義(HR=2.048,95%CI 1.00-4.19,P=0.05)。OLC1在年齡≥60歲的患者中更易出現(xiàn)過表達(dá)(P=0.002),OLC1與年齡≥60歲共同作為危險因素時,患者的預(yù)后顯著變差(HR=5.125,95%CI 1.756-14.96,P=0.003)。結(jié)論OLC1在腺癌組織中的過表達(dá)率明顯高于鱗癌,但OLC1在癌組織中的過表達(dá)與腺癌患者的預(yù)后關(guān)系不明顯;OLC1在≥60歲的鱗癌患者組織中更易表達(dá),并可能與年齡一起共同影響鱗癌患者的預(yù)后,但在癌組織中的過表達(dá)不足以作為影響肺鱗癌患者預(yù)后的獨立危險因素。
[Abstract]:Background & objective OLC1 (overexpressed in lung cancer 1 is a novel lung cancer associated gene. Previous studies have found that it is highly expressed in squamous cell carcinoma, adenocarcinoma and small cell carcinoma. The expression of OLC1 protein in peripheral plasma of lung cancer patients was significantly higher than that in normal controls, and could be used as a candidate marker for auxiliary diagnosis of lung cancer. OLC1 was also highly expressed in esophageal squamous cell carcinoma, ovarian cancer, breast cancer and colorectal cancer. But the relationship between OLC1 and prognosis of lung cancer patients has not been studied. In this study, we examined the expression of OLC1 protein in non-small cell lung cancer (NSCLC) patients with (NSCLC), and analyzed the relationship between the overexpression of OLC1 and the prognosis of NSCLC patients. Methods the tissue microarray of 75 cases of squamous cell carcinoma and 90 cases of adenocarcinoma and 33 cases of squamous cell carcinoma were stained with immunohistochemical staining to detect the expression of OLC1 protein. To analyze the relationship between its expression and clinical features and prognosis. Results the overexpression rate of OLC1 in adenocarcinoma (87.5%) was significantly higher than that in squamous cell carcinoma (55.3p0.001). There was no significant correlation between the overexpression of OLC1 and prognosis in adenocarcinoma. Overexpression in squamous cell carcinoma was associated with poor prognosis. Univariate Kaplan-Meier survival analysis suggested that patients with overexpression of OLC1 had a worse prognosis (P0. 042). Multivariate Cox regression analysis showed that there was no significant difference in survival between the two groups (HR=2.048,95%CI 1.00-4.19 P0. 05). OLC1 was more likely to be expressed in patients with age 鈮，

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