【摘要】：[目的]本研究通過檢測宣威肺癌、非宣威肺癌、宣威肺良性病、健康對照四組人群血漿中FENDRR、H19基因的表達水平,研究FENDRR、H19在宣威肺癌患者血漿中的變化趨勢及臨床意義,評價FENDRR、H19在宣威肺癌患者中的潛在診斷價值。[方法]收集2015年6月至2016年8月昆明醫(yī)科大學第一附屬醫(yī)院患者術前以及性別年齡匹配的體檢健康者血漿,共163例樣本。樣本分宣威肺癌、非宣威肺癌、宣威良性肺疾病、健康對照4組,其中宣威肺癌43例,其它3組每組各40例。采用實時熒光定量PCR方法檢測研究對象血漿中FENDRR、H19基因的表達水平。并對宣威肺癌患者臨床病例資料進行統(tǒng)計分析,分析FENDRR、H19表達水平與患者性別、年齡、吸煙情況、TNM分期、淋巴結轉移情況、腫塊大小和組織學類型的相關性,評價血漿FENDRR和H19作為宣威肺癌篩查標志物的診斷價值。[結果]與對照組相比,FENDRR、H19在宣威肺癌組、非宣威肺癌組中表達水平整體呈下調趨勢(P0.05)。FENDRR、H19的表達水平與患者性別、年齡、吸煙史、TNM分期、組織學類型、腫塊大小均無相關性(P0.05);H19的表達水平與患者淋巴結轉移情況有相關性(P0.05)。NSE的AUC值為0.648,敏感性和特異性分別為52.6%和78.9%;CEA的AUC值為0.759,敏感性和特異性分別為68.4%和 73.7%;聯(lián)合檢測 NSE 和 CEA 時,AUC 值為 0.778。FENDRR的AUC值為 0.812,敏感性和特異性分別為90.7%和75%;H19的AUC值為0.833,敏感性和特異性分別為74.4%和85%;而當聯(lián)合檢測FENDRR和H19時,AUC值為0.894。[結論]FENDRR、H19在宣威肺癌的發(fā)生、發(fā)展中發(fā)揮著重要作用,它們可以作為宣威肺癌潛在的診斷標志物,而且診斷價值優(yōu)于傳統(tǒng)標志物。
[Abstract]:[objective] to investigate the expression of FENDRR,H19 gene in the plasma of Xuanwei lung cancer, non-Xuanwei lung cancer, Xuanwei lung benign venereal disease and healthy control group, and to study the change trend and clinical significance of FENDRR,H19 in the plasma of Xuanwei lung cancer patients. To evaluate the potential diagnostic value of FENDRR,H19 in Xuanwei lung cancer patients. Methods from June 2015 to August 2016, a total of 163 plasma samples were collected from patients in the first affiliated Hospital of Kunming Medical University. The samples were divided into 4 groups: Xuanwei lung cancer, non-Xuanwei lung cancer, Xuanwei benign lung disease, healthy control group, 43 cases of Xuanwei lung cancer, 40 cases each of the other three groups. Real-time fluorescence quantitative PCR was used to detect the expression of FENDRR,H19 gene in plasma. The correlation between FENDRR,H19 expression and sex, age, smoking status, TNM stage, lymph node metastasis, tumor size and histological type was analyzed. To evaluate the diagnostic value of plasma FENDRR and H 19 as screening markers of Xuan Wei lung cancer. [results] compared with the control group, the expression of FENDRR,H19 in Xuanwei lung cancer group and non-Xuanwei lung cancer group showed a downward trend (P0.05). The expression level of FENDRR,H19 was correlated with sex, age, smoking history, TNM stage and histological type. There was no correlation between tumor size and tumor size (P0.05). The expression of H19 was correlated with lymph node metastasis (P0.05 AUC value of). NSE was 0.648, sensitivity and specificity were 52.6% and 78.9%, respectively); The AUC value of CEA was 0.759, the sensitivity and specificity were 68.4% and 73.7%, the AUC value of 0.778.FENDRR was 0.812, the sensitivity and specificity were 90.7% and 75%, respectively. The AUC value of H19 was 0.833, the sensitivity and specificity of H19 were 74.4% and 85.When combined detection of FENDRR and H19, the AUC value was 0.894. [conclusion] FENDRR,H19 plays an important role in the occurrence and development of Xuanwei lung cancer. They can be used as potential diagnostic markers of Xuanwei lung cancer, and the diagnostic value is superior to that of traditional markers.
【學位授予單位】：昆明醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R734.2

【參考文獻】

相關期刊論文 前9條

1 李繼華;何俊;唐銳;Wei HU;Qing LAN;何興舟;李云;張云生;;宣威肺癌分子流行病學研究:煤種、基因型與肺癌風險[J];中國肺癌雜志;2015年01期

2 屈若yN;周寶森;;2004-2010年中國肺癌死亡分布及趨勢分析[J];中國衛(wèi)生統(tǒng)計;2014年06期

3 李慶;王玉明;段勇;;長鏈非編碼RNA與腫瘤關系的研究現狀[J];實驗與檢驗醫(yī)學;2014年04期

4 靳國偉;韓世波;陳躍平;;神經元特異性烯醇化酶的臨床應用研究進展[J];中國臨床神經外科雜志;2013年03期

5 史華俊;劉忠;郭俊明;;非編碼長鏈RNA與基因表達調控[J];中國生物化學與分子生物學報;2012年09期

6 熊杰;白生華;徐萬清;古宇;龔楊彬;;血清腫瘤標志物聯(lián)合檢測對肺癌的診斷價值[J];現代腫瘤醫(yī)學;2012年03期

7 丁湘_g;張寶秋;張潔;王雪玉;尹顏軍;榮長利;時廣利;;肺癌患者血清中腫瘤標志物檢測的臨床意義[J];中華臨床醫(yī)師雜志(電子版);2011年16期

8 陳青;鄔黎青;;p53基因和乳腺癌的相關性研究進展[J];實驗與檢驗醫(yī)學;2011年01期

9 黃云超;趙光強;肖義澤;陳小波;馬千里;雷玉潔;楊凱云;;云南肺癌高發(fā)地區(qū)肺癌死亡趨勢分析[J];腫瘤防治研究;2011年01期

，

本文編號：2364290