【摘要】：目的:通過與化療藥物比較的實(shí)驗(yàn)研究探討大蒜素對(duì)食管癌的抗腫瘤效果及其可能機(jī)制。方法:不同濃度的大蒜素、5-氟尿嘧啶和順鉑作用于食管癌細(xì)胞EC109,分別在6 h、12 h、24 h、48 h和72 h應(yīng)用CCK-8法檢測(cè)EC109細(xì)胞的生長(zhǎng)抑制率,并以分析細(xì)胞乳酸脫氫酶(LDH)活性反映各藥物的細(xì)胞毒性;Annexin V/PI雙染法檢測(cè)空白對(duì)照組、Z-VAD-FMK(caspase-3活性抑制劑)組、大蒜素組、大蒜素+Z-VAD-FMK組、5-氟尿嘧啶組和順鉑組的細(xì)胞凋亡情況。分光光度測(cè)定法檢測(cè)細(xì)胞內(nèi)caspase-3、8、9的活性變化。結(jié)果:大蒜素呈濃度依賴性及時(shí)間依賴性抑制和殺傷食管癌細(xì)胞EC109;與5-氟尿嘧啶組和順鉑組比較,大蒜素組LDH活性明顯下降;與對(duì)照組相比,大蒜素培養(yǎng)細(xì)胞內(nèi)caspase-3、8的活性增強(qiáng),而細(xì)胞內(nèi)caspase-9的活性變化差異無統(tǒng)計(jì)學(xué)顯著性。結(jié)論:大蒜素可能通過活化caspase-8激活外源性凋亡通路,誘導(dǎo)食管癌細(xì)胞EC109凋亡,從而抑制腫瘤細(xì)胞的生長(zhǎng),且與5-氟尿嘧啶和順鉑相比,大蒜素的細(xì)胞毒副作用明顯減弱。
[Abstract]:Aim: to investigate the antitumor effect of allicin on esophageal carcinoma and its possible mechanism by comparing with chemotherapeutic drugs. Methods: different concentrations of allicin, 5-fluorouracil and cisplatin were used to detect the growth inhibition rate of EC109 cells in EC109, cells at 6 h, 12 h, 24 h, 48 h and 72 h, respectively. The activity of lactate dehydrogenase (LDH) was analyzed to reflect the cytotoxicity of various drugs. Annexin V/PI double staining method was used to detect apoptosis in control group, Z-VAD-FMK (caspase-3 activity inhibitor) group, allicin Z-VAD-FMK group, 5-fluorouracil group and cisplatin group. The activity of caspase-3,8,9 was detected by spectrophotometry. Results: compared with 5-fluorouracil group and cisplatin group, the LDH activity of allicin group decreased significantly compared with 5-fluorouracil group and cisplatin group. Compared with the control group, the activity of caspase-3,8 was increased in allicin culture cells, but there was no significant difference in the activity of caspase-9 in the cells. Conclusion: allicin may activate exogenous apoptosis pathway by activating caspase-8 and induce EC109 apoptosis in esophageal carcinoma cells, thus inhibiting the growth of tumor cells, and the cytotoxic side effects of allicin are significantly reduced compared with 5-fluorouracil and cisplatin.
【作者單位】： 福建醫(yī)科大學(xué)附屬龍巖第一醫(yī)院心胸外科;福建醫(yī)科大學(xué)附屬龍巖第一醫(yī)院重癥醫(yī)學(xué)科;
【基金】：福建省衛(wèi)生廳青年科研基金資助項(xiàng)目(No.2013-2-150) 福建醫(yī)科大學(xué)非直屬附屬醫(yī)院科學(xué)研究專項(xiàng)基金資助項(xiàng)目(No.FZS13013Y)
【分類號(hào)】：R735.1

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