【摘要】：目的:通過與化療藥物比較的實驗研究探討大蒜素對食管癌的抗腫瘤效果及其可能機(jī)制。方法:不同濃度的大蒜素、5-氟尿嘧啶和順鉑作用于食管癌細(xì)胞EC109,分別在6 h、12 h、24 h、48 h和72 h應(yīng)用CCK-8法檢測EC109細(xì)胞的生長抑制率,并以分析細(xì)胞乳酸脫氫酶(LDH)活性反映各藥物的細(xì)胞毒性;Annexin V/PI雙染法檢測空白對照組、Z-VAD-FMK(caspase-3活性抑制劑)組、大蒜素組、大蒜素+Z-VAD-FMK組、5-氟尿嘧啶組和順鉑組的細(xì)胞凋亡情況。分光光度測定法檢測細(xì)胞內(nèi)caspase-3、8、9的活性變化。結(jié)果:大蒜素呈濃度依賴性及時間依賴性抑制和殺傷食管癌細(xì)胞EC109;與5-氟尿嘧啶組和順鉑組比較,大蒜素組LDH活性明顯下降;與對照組相比,大蒜素培養(yǎng)細(xì)胞內(nèi)caspase-3、8的活性增強(qiáng),而細(xì)胞內(nèi)caspase-9的活性變化差異無統(tǒng)計學(xué)顯著性。結(jié)論:大蒜素可能通過活化caspase-8激活外源性凋亡通路,誘導(dǎo)食管癌細(xì)胞EC109凋亡,從而抑制腫瘤細(xì)胞的生長,且與5-氟尿嘧啶和順鉑相比,大蒜素的細(xì)胞毒副作用明顯減弱。
[Abstract]:Aim: to investigate the antitumor effect of allicin on esophageal carcinoma and its possible mechanism by comparing with chemotherapeutic drugs. Methods: different concentrations of allicin, 5-fluorouracil and cisplatin were used to detect the growth inhibition rate of EC109 cells in EC109, cells at 6 h, 12 h, 24 h, 48 h and 72 h, respectively. The activity of lactate dehydrogenase (LDH) was analyzed to reflect the cytotoxicity of various drugs. Annexin V/PI double staining method was used to detect apoptosis in control group, Z-VAD-FMK (caspase-3 activity inhibitor) group, allicin Z-VAD-FMK group, 5-fluorouracil group and cisplatin group. The activity of caspase-3,8,9 was detected by spectrophotometry. Results: compared with 5-fluorouracil group and cisplatin group, the LDH activity of allicin group decreased significantly compared with 5-fluorouracil group and cisplatin group. Compared with the control group, the activity of caspase-3,8 was increased in allicin culture cells, but there was no significant difference in the activity of caspase-9 in the cells. Conclusion: allicin may activate exogenous apoptosis pathway by activating caspase-8 and induce EC109 apoptosis in esophageal carcinoma cells, thus inhibiting the growth of tumor cells, and the cytotoxic side effects of allicin are significantly reduced compared with 5-fluorouracil and cisplatin.
【作者單位】： 福建醫(yī)科大學(xué)附屬龍巖第一醫(yī)院心胸外科;福建醫(yī)科大學(xué)附屬龍巖第一醫(yī)院重癥醫(yī)學(xué)科;
【基金】：福建省衛(wèi)生廳青年科研基金資助項目(No.2013-2-150) 福建醫(yī)科大學(xué)非直屬附屬醫(yī)院科學(xué)研究專項基金資助項目(No.FZS13013Y)
【分類號】：R735.1

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