法舒地爾保護阿霉素對心肌損害的實驗研究
發(fā)布時間：2018-09-16 19:48
【摘要】：目的:研究阿霉素聯(lián)合法舒地爾在白血病患者化療過程中對K562細胞的殺傷作用以及對人心肌細胞的保護作用。方法:選取對數(shù)期生長的K562細胞及HCM細胞,分別對其進行藥物干預,設(shè)立對照組A1及A2,單用阿霉素組B1及B2,阿霉素聯(lián)合法舒地爾組C1及C2,阿霉素聯(lián)合右丙亞胺組D1及D2(陽性對照組),干預后的細胞分別在倒置顯微鏡下通過檢測細胞的形態(tài),MTT比色法檢測細胞活性,流式檢測細胞凋亡率,western blot檢測細胞中抑制凋亡基因Bcl-2和促凋亡基因Bax的表達情況。結(jié)果:K562細胞實驗的結(jié)果為:倒置顯微鏡下與對照組A1相比,實驗組B1、C1、D1細胞拒染實驗均為陽性;MTT比色法測細胞活性示與對照組A1相比,實驗組B1、C1、D1細胞活性均降低,且通過吸光度散點圖可以看出三組實驗組K562細胞吸光度相近;流式檢測細胞凋亡率示與對照組A1((?)=4.93%)相比,實驗組B1((?)=81.9%)、C1((?)=78.3%)、D1((?)=86.0%)細胞凋亡率明顯升高,且三組間凋亡率差異不大;western條帶圖示對照組A1及實驗組B1、C1、D1的內(nèi)參條帶β-Actin強度基本相等,抑凋亡基因Bcl-2條帶強度在對照組A1強度較高,在三組實驗組B1、C1、D1條帶強度較A1組低,促凋亡基因Bax的條帶強度在對照組A1第,在三組實驗組B1、C1、D1條帶強度較A1組高。HCM細胞實驗結(jié)果為:倒置顯微鏡下與對照組A2相比,實驗組B2、C2、D2細胞拒染實驗均為陽性,且鏡下C2、D2組細胞形態(tài)較B2組細胞形態(tài)變化較少;MTT比色法測細胞活性示與對照組A2相比,實驗組B2、C2、D2細胞活性均降低,且通過吸光度散點圖可以看出C2、D2組HCM細胞吸光度相近并高于B2組;流式檢測細胞凋亡率示與對照組A1((?)=0.13%)相比,實驗組B1((?)=42.5%)、C1((?)=24.1%)、D1((?)=23.7%)細胞凋亡率明顯升高,且C2、D2組間凋亡率相近并低于B2組;western條帶圖示對照組A2及實驗組B2、C2、D2的內(nèi)參條帶β-Actin強度基本相等,抑凋亡基因Bcl-2條帶強度在對照組A1強度最高,其次為C2及D2組,在B2組條帶強度最低,促凋亡基因Bax的條帶強度在對照組A2最低,在三組實驗組B2、C2、D2條帶強度較A2增高,C2及D2組條帶較高,B2組條帶強度最高。結(jié)論:在白血病患者化療過程中,阿霉素聯(lián)合法舒地爾無明顯藥物拮抗作用,聯(lián)合用藥在不降低化療效果的基礎(chǔ)上對心肌細胞有一定的保護作用。
[Abstract]:Aim: to study the cytotoxicity of doxorubicin combined with fasudil on K562 cells and its protective effect on human cardiomyocytes during chemotherapy. Methods: K562 cells and HCM cells grew in logarithmic phase were treated with drugs respectively. Control group A1 and A2, adriamycin group B1 and B2, adriamycin combined with fasudil group C1 and C2, adriamycin combined with dextromine group D1 and D2 (positive control group) were used to detect the cells under inverted microscope. The cell activity was detected by MTT colorimetric assay. Apoptosis rate was detected by flow cytometry and western blot was used to detect the expression of Bcl-2 and Bax. Results the results of the cell experiment were as follows: compared with the control group A1 under inverted microscope, the cell activity of the experimental group was decreased by MTT colorimetric assay, while that of the control group was decreased by MTT colorimetric assay compared with that of the control group, and the results showed that the activity of the cells in the experimental group was lower than that in the control group, and that in the control group was significantly lower than that in the control group. The results showed that the absorbance of K562 cells in the three experimental groups was similar to that in the control group, and the apoptotic rate of K562 cells in the experimental group was significantly higher than that in the control group A1 (?) 4.93%), B _ 1 (?) 81.9%), C _ 1 (?) 78.3%) and D _ 1 (?) 86.0%), compared with the control group (A _ 1 (?) 4.93%). There was no significant difference in apoptosis rate among the three groups. The intensity of 尾 -Actin bands in the control group A1 and the experimental group B1C1C1nD1 was basically equal. The intensity of the Bcl-2 band of anti-apoptotic gene was higher in the control group than that in the control group, and the intensity of the B1C1C1mD1 band in the three experimental groups was lower than that in the A1 group. The band intensity of apoptosis-promoting gene Bax was higher in control group A1 than that in A1 group. The results were as follows: compared with control group A _ 2 under inverted microscope, B _ 2C _ 2T _ 2 cells in experimental group were positive in staining rejection test. Under microscope, the changes of cell morphology in group C _ 2 were less than those in group B _ 2. MTT assay showed that the activity of B _ 2 C _ 2O _ 2 cells in the experimental group was lower than that in the control group (A _ 2). The results showed that the absorbance of HCM cells in C _ 2O _ 2 group was similar and higher than that in B _ 2 group, and the apoptotic rate was significantly higher in experimental group B1 (?) 42.5%) than that in control group A1 (?) 0.13%), C _ 1 (?) 24.1%) and D _ 1 (?) 23.7%) in the experimental group, and the apoptosis rate was significantly higher in the C _ 2O _ 2 group than in the control group (A _ 1 (?) 0.13%). The intensity of 尾 -Actin in B _ 2 and B _ 2C _ 2N _ 2 of the control group and the experimental group was basically equal. The intensity of the Bcl-2 band of anti-apoptotic gene was the highest in control group A1, followed by C2 and D2 groups. In B2 group, the band intensity of apoptotic gene Bax was the lowest, and the intensity of B _ 2 C _ 2C _ 2 D _ 2 band in three experimental groups was higher than that of A _ 2 and C _ 2 and D _ 2 groups, and the intensity of B _ 2 band in B _ 2 group was higher than that in B _ 2 group and B _ 2 group was higher than that in D _ 2 group. Conclusion: doxorubicin combined with fasudil has no obvious antagonistic effect during chemotherapy in leukemia patients. The combination of adriamycin and fasudil has a protective effect on cardiomyocytes on the basis of not reducing the effect of chemotherapy.
【學位授予單位】：西安醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R733.7
本文編號：2244603
[Abstract]:Aim: to study the cytotoxicity of doxorubicin combined with fasudil on K562 cells and its protective effect on human cardiomyocytes during chemotherapy. Methods: K562 cells and HCM cells grew in logarithmic phase were treated with drugs respectively. Control group A1 and A2, adriamycin group B1 and B2, adriamycin combined with fasudil group C1 and C2, adriamycin combined with dextromine group D1 and D2 (positive control group) were used to detect the cells under inverted microscope. The cell activity was detected by MTT colorimetric assay. Apoptosis rate was detected by flow cytometry and western blot was used to detect the expression of Bcl-2 and Bax. Results the results of the cell experiment were as follows: compared with the control group A1 under inverted microscope, the cell activity of the experimental group was decreased by MTT colorimetric assay, while that of the control group was decreased by MTT colorimetric assay compared with that of the control group, and the results showed that the activity of the cells in the experimental group was lower than that in the control group, and that in the control group was significantly lower than that in the control group. The results showed that the absorbance of K562 cells in the three experimental groups was similar to that in the control group, and the apoptotic rate of K562 cells in the experimental group was significantly higher than that in the control group A1 (?) 4.93%), B _ 1 (?) 81.9%), C _ 1 (?) 78.3%) and D _ 1 (?) 86.0%), compared with the control group (A _ 1 (?) 4.93%). There was no significant difference in apoptosis rate among the three groups. The intensity of 尾 -Actin bands in the control group A1 and the experimental group B1C1C1nD1 was basically equal. The intensity of the Bcl-2 band of anti-apoptotic gene was higher in the control group than that in the control group, and the intensity of the B1C1C1mD1 band in the three experimental groups was lower than that in the A1 group. The band intensity of apoptosis-promoting gene Bax was higher in control group A1 than that in A1 group. The results were as follows: compared with control group A _ 2 under inverted microscope, B _ 2C _ 2T _ 2 cells in experimental group were positive in staining rejection test. Under microscope, the changes of cell morphology in group C _ 2 were less than those in group B _ 2. MTT assay showed that the activity of B _ 2 C _ 2O _ 2 cells in the experimental group was lower than that in the control group (A _ 2). The results showed that the absorbance of HCM cells in C _ 2O _ 2 group was similar and higher than that in B _ 2 group, and the apoptotic rate was significantly higher in experimental group B1 (?) 42.5%) than that in control group A1 (?) 0.13%), C _ 1 (?) 24.1%) and D _ 1 (?) 23.7%) in the experimental group, and the apoptosis rate was significantly higher in the C _ 2O _ 2 group than in the control group (A _ 1 (?) 0.13%). The intensity of 尾 -Actin in B _ 2 and B _ 2C _ 2N _ 2 of the control group and the experimental group was basically equal. The intensity of the Bcl-2 band of anti-apoptotic gene was the highest in control group A1, followed by C2 and D2 groups. In B2 group, the band intensity of apoptotic gene Bax was the lowest, and the intensity of B _ 2 C _ 2C _ 2 D _ 2 band in three experimental groups was higher than that of A _ 2 and C _ 2 and D _ 2 groups, and the intensity of B _ 2 band in B _ 2 group was higher than that in B _ 2 group and B _ 2 group was higher than that in D _ 2 group. Conclusion: doxorubicin combined with fasudil has no obvious antagonistic effect during chemotherapy in leukemia patients. The combination of adriamycin and fasudil has a protective effect on cardiomyocytes on the basis of not reducing the effect of chemotherapy.
【學位授予單位】：西安醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R733.7
【參考文獻】
相關(guān)期刊論文 前10條
1 ;CA:2015中國癌癥統(tǒng)計數(shù)據(jù)發(fā)布[J];中國醫(yī)學創(chuàng)新;2016年05期
2 董文哲;阮林;何并文;彭丹暉;文韜;梁新強;杜學柯;陳肖東;;法舒地爾對阿霉素致大鼠心肌損傷的保護作用[J];中國癌癥防治雜志;2015年03期
3 王璇;;右丙亞胺聯(lián)合參麥注射液對治療急性白血病患者化療期間心臟毒性的研究[J];中國民康醫(yī)學;2015年12期
4 陳華;呂妍琨;齊鵬;杜榮品;谷劍;陳淑霞;耿彥平;李燕;于芳;;Rho激酶信號通路與大鼠心肌細胞凋亡的關(guān)系[J];中國老年學雜志;2015年06期
5 張培勇;沈思鈺;蔡輝;趙智明;;法舒地爾對大鼠心肌血管緊張素轉(zhuǎn)換酶2和血管緊張素(1-7)的影響[J];中華老年心腦血管病雜志;2013年05期
6 唐祖勝;屈紅;劉偉;;法舒地爾治療老年舒張性左心衰竭相關(guān)性肺動脈高壓的有效性及安全性研究[J];中國全科醫(yī)學;2012年17期
7 馬紅梅;涂傳清;;蒽環(huán)類藥物心臟毒性機制及其監(jiān)測進展[J];醫(yī)學綜述;2010年08期
8 車菲菲;劉瑜;徐才剛;;右丙亞胺對阿霉素引起的心臟毒性防治效果及其機制研究[J];四川大學學報(醫(yī)學版);2010年01期
9 張競競;孫惠娟;;蒽環(huán)類抗腫瘤藥物的心臟毒性[J];臨床肺科雜志;2008年03期
10 易善永;南克俊;陳圣杰;盧創(chuàng)新;孔鶯;;銀杏葉提取物對多柔比星所致乳腺癌患者心臟毒性的干預作用[J];中國中西醫(yī)結(jié)合雜志;2008年01期
，本文編號：2244603
本文鏈接：http://sikaile.net/yixuelunwen/zlx/2244603.html
最近更新
教材專著
