子宮內(nèi)膜癌組織辣椒素受體TRPV1表達(dá)臨床意義探討
發(fā)布時(shí)間：2018-09-12 10:05
【摘要】：目的辣椒素受體瞬時(shí)受體電位香草酸亞型1(transient receptor potential vanilloid 1,TRPV1)參與了多種腫瘤的生理和病理過程,但TRPV1在子宮內(nèi)膜癌組織中的表達(dá)鮮見報(bào)道。本研究旨在觀察子宮內(nèi)膜癌組織及細(xì)胞中TRPV1的表達(dá)情況,并探討其意義。方法收集河北醫(yī)科大學(xué)第二醫(yī)院2009-11-02-2015-08-31子宮內(nèi)膜癌組織蠟塊73例。對(duì)照組選取正常增殖期宮內(nèi)膜10例;子宮內(nèi)膜單純或復(fù)雜性增生10例;子宮內(nèi)膜非典型增生10例。免疫組化SP法測(cè)定TRPV1在不同類型子宮內(nèi)膜癌組織中的表達(dá),蛋白質(zhì)印跡法檢測(cè)人子宮內(nèi)膜癌細(xì)胞系ISHIKAWA和HEC-1A的中TRPV1表達(dá)情況。結(jié)果免疫組化結(jié)果顯示,TRPV1在子宮內(nèi)膜癌組織的陽(yáng)性表達(dá)率為42.5%(31/73),較正常內(nèi)膜表達(dá)率100%(10/10)明顯降低,χ~2=31.583,P0.001。其中Ⅰ型子宮內(nèi)膜癌組織的陽(yáng)性表達(dá)率為73.5%(25/34),高于Ⅱ型子宮內(nèi)膜癌的表達(dá)率15.4%(6/39),Z=-5.669,P0.001。蛋白質(zhì)印跡法結(jié)果顯示,ER高表達(dá)的ISHIKAWA癌細(xì)胞系表達(dá)TRPV1蛋白,而ER低表達(dá)的HEC-1A癌細(xì)胞系未檢測(cè)到TRPV1蛋白表達(dá)。結(jié)論Ⅰ型子宮內(nèi)膜癌的發(fā)生發(fā)展可能有TRPV1參與,Ⅱ型子宮內(nèi)膜癌的發(fā)生可能與TRPV1的丟失有關(guān)。子宮內(nèi)膜癌中TRPV1的表達(dá)水平可能與ER的表達(dá)相關(guān)聯(lián)。
[Abstract]:Objective capsaicin receptor transient receptor subtype 1 (transient receptor potential vanilloid 1 / TRPV1) is involved in the physiological and pathological processes of various tumors, but the expression of TRPV1 in endometrial carcinoma is rarely reported. The purpose of this study was to investigate the expression and significance of TRPV1 in endometrial carcinoma tissues and cells. Methods 73 cases of endometrial carcinoma were collected from the second Hospital of Hebei Medical University 2009-11-02-2015-08-31. In the control group, 10 cases of normal proliferative endometrium, 10 cases of simple or complex hyperplasia of endometrium and 10 cases of atypical hyperplasia of endometrium were selected. Immunohistochemical SP method was used to detect the expression of TRPV1 in different types of endometrial carcinoma. Western blot was used to detect the expression of TRPV1 in human endometrial carcinoma cell line ISHIKAWA and HEC-1A. Results Immunohistochemical results showed that the positive rate of TRPV1 in endometrial carcinoma was 42.5% (31 / 73), which was significantly lower than that in normal endometrium (100%, 10 / 10). The positive expression rate of type 鈪，
本文編號(hào)：2238674
[Abstract]:Objective capsaicin receptor transient receptor subtype 1 (transient receptor potential vanilloid 1 / TRPV1) is involved in the physiological and pathological processes of various tumors, but the expression of TRPV1 in endometrial carcinoma is rarely reported. The purpose of this study was to investigate the expression and significance of TRPV1 in endometrial carcinoma tissues and cells. Methods 73 cases of endometrial carcinoma were collected from the second Hospital of Hebei Medical University 2009-11-02-2015-08-31. In the control group, 10 cases of normal proliferative endometrium, 10 cases of simple or complex hyperplasia of endometrium and 10 cases of atypical hyperplasia of endometrium were selected. Immunohistochemical SP method was used to detect the expression of TRPV1 in different types of endometrial carcinoma. Western blot was used to detect the expression of TRPV1 in human endometrial carcinoma cell line ISHIKAWA and HEC-1A. Results Immunohistochemical results showed that the positive rate of TRPV1 in endometrial carcinoma was 42.5% (31 / 73), which was significantly lower than that in normal endometrium (100%, 10 / 10). The positive expression rate of type 鈪，
本文編號(hào)：2238674
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