【摘要】：目的探討超聲微泡造影劑介導miRNA質(zhì)粒轉(zhuǎn)染人肝癌HepG2細胞后遷移、侵襲及克隆能力的改變。方法在前期實驗所篩選的最佳超聲微泡轉(zhuǎn)染條件下,轉(zhuǎn)染目的基因反義miR-21/221、miR-199a進入人肝癌HepG2細胞,劃痕實驗、Transwell實驗檢測細胞遷移、侵襲能力,軟瓊脂克隆形成實驗檢測細胞克隆能力。結(jié)果轉(zhuǎn)染目的質(zhì)粒后,細胞的遷移、侵襲能力及克隆能力與對照組比較均顯著受到抑制(P0.05),其中以miR-199a質(zhì)粒組的抑制效果最佳(相對細胞遷移率31.05%;平均視野侵襲細胞數(shù)38.67±4.51;克隆數(shù)105.67±5.86),與其它目的質(zhì)粒組比較,差異均有統(tǒng)計學意義(P0.05)。結(jié)論通過分析miRNAs對人肝癌HepG2細胞部分細胞功能的影響,為肝癌的基因治療提供新的靶點及思路。
[Abstract]:Objective to investigate the migration, invasion and clone ability of human hepatocellular carcinoma (HepG2) cells transfected with miRNA plasmid by ultrasound microbubble contrast medium. Methods under the optimal ultrasound microbubble transfection conditions, the target gene antisense miR-21/221,miR-199a was transfected into human hepatoma HepG2 cells. Soft Agar Clone formation Test was used to detect the ability of cell clone. Results after transfection of the target plasmid, cell migration was observed. The invasiveness and clone ability of miR-199a plasmid group were significantly inhibited compared with the control group (P0.05). The inhibitory effect of miR-199a plasmid group was the best (relative cell migration rate 31.05; average visual field invasion cell number 38.67 鹵4.51; clone number 105.67 鹵5.86). The difference was statistically significant (P0.05). Conclusion by analyzing the effect of miRNAs on the function of human hepatoma HepG2 cells, we can provide new targets and ideas for gene therapy of HCC.
【作者單位】： 暨南大學醫(yī)學院附屬廣州市紅會醫(yī)院超聲診斷科;廣東藥學院附屬廣鋼醫(yī)院超聲診斷科;
【基金】：廣東省科技計劃項目立項(編號:2016A020215015)
【分類號】：R735.7

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