【摘要】：研究背景結(jié)直腸癌(Colorectal cancer)在我國發(fā)病率逐漸增高,因其較高的侵襲和轉(zhuǎn)移特性,結(jié)直腸癌在癌癥相關(guān)的死亡中占據(jù)第二位。超過30%的結(jié)直腸癌患者在自然情況下會進展成轉(zhuǎn)移性結(jié)直腸癌,而轉(zhuǎn)移性結(jié)直腸癌的中位生存時間小于30個月。因此,早期預(yù)測結(jié)直腸癌惡性進展以采取相應(yīng)治療措施,是提高結(jié)直腸癌患者生存期的有效方法。微小RNA(microRNA, miRNA),是一組高度保守的單鏈非編碼RNA,它可通過靶向結(jié)合的方式特異性的結(jié)合到其下游靶基因mRNA的3'UTR區(qū)來調(diào)節(jié)其轉(zhuǎn)錄或降解,從而影響細胞增殖、凋亡、侵襲等生物學(xué)行為,表現(xiàn)為促進或抑制腫瘤發(fā)生。目的探討微小RNA-100(miR-100)在人結(jié)直腸癌組織內(nèi)表達情況與病理特征及患者預(yù)后的關(guān)系。方法采用實時定量PCR方法檢測172例結(jié)直腸癌組織及癌旁組織內(nèi)miR-100的表達水平,并分析其與淋巴結(jié)轉(zhuǎn)移、分化程度、TNM分期及預(yù)后的關(guān)系。在體外試驗中,構(gòu)建miR-100高表達結(jié)直腸癌細胞系HCT-8-miR-100及對照組HCT-8-NC,觀察其對結(jié)直腸癌細胞增殖、凋亡和遷移的影響。結(jié)果miR-100在結(jié)直腸癌組織中表達低于癌旁對照組織(P0.01)。結(jié)直腸癌組織中有淋巴結(jié)轉(zhuǎn)移組miR-100表達直于無淋巴結(jié)轉(zhuǎn)移組(P0.01)；從TNM分期Ⅰ期到Ⅳ期,miR-100表達量逐浙增加(P0.01)；從病理分化程度好到分化程度差,miR-100表達量逐漸增加(P0.01)。生存分析顯示,miR-100高表達組與低表達組相比總生存率呈下降趨勢,但差異無統(tǒng)計學(xué)意義(P=0.179)；單因素生存分析顯示淋巴結(jié)轉(zhuǎn)移、病理分化程度、TNM分期,是影響預(yù)后的預(yù)測因子；體外實驗表明,高表達miR-100明顯抑制細胞增殖,促進凋亡及遷移。結(jié)論miR-100表達升高與淋巴結(jié)轉(zhuǎn)移、分化程度及TNM分期有關(guān)。MiR-100可能作為提示結(jié)直腸癌淋巴結(jié)轉(zhuǎn)移及惡性進展的潛在標志物。
[Abstract]:Background the incidence of (Colorectal cancer) in colorectal cancer is increasing in China. Because of its high invasion and metastasis, colorectal cancer occupies the second place in cancer related deaths. More than 30% of patients with colorectal cancer naturally develop metastatic colorectal cancer, and the median survival time of metastatic colorectal cancer is less than 30 months. Therefore, early prediction of malignant progression of colorectal cancer and appropriate treatment measures are effective methods to improve the survival time of colorectal cancer patients. Small RNA (microRNA, miRNA), is a group of highly conserved single-strand non-coding RNA, that can specifically bind to the 3'UTR region of its downstream target gene mRNA to regulate its transcription or degradation, thus affecting cell proliferation, apoptosis, invasion and other biological behaviors. Its manifestation is to promote or suppress the occurrence of tumor. Objective to investigate the relationship between the expression of micro RNA-100 (miR-100) in human colorectal carcinoma and its pathological features and prognosis. Methods Real-time quantitative PCR was used to detect the expression of miR-100 in 172 colorectal cancer tissues and adjacent tissues, and the relationship between miR-100 expression and lymph node metastasis, differentiation and prognosis was analyzed. In vitro, the effects of miR-100 overexpression on the proliferation, apoptosis and migration of colorectal cancer cell line HCT-8-miR-100 and control group HCT-8-NC, were observed. Results the expression of miR-100 in colorectal cancer tissues was lower than that in adjacent control tissues (P0.01). The expression of miR-100 in colorectal cancer with lymph node metastasis was higher than that without lymph node metastasis (P0.01), the expression of miR-100 was increased gradually from stage 鈪，

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