【摘要】：目的利用免疫組織化學(xué)方法(PV9000兩步法)檢測ErbB2與Ebpl蛋白在胃癌組織的表達(dá)情況,分析ErbB2與Ebp1蛋白的表達(dá)與胃癌臨床病理特征之間的關(guān)系及二者在胃癌組織中表達(dá)的相關(guān)性。方法收集2011年1月至2013年11月于延邊大學(xué)附屬醫(yī)院行手術(shù)治療后送檢的胃癌組織標(biāo)本132例,其中,男性97例,女性35例：漢族85例,朝鮮族47例。年齡在38歲至81歲之間,平均年齡為63.03±9.06歲,其中,年齡60歲者42例,年齡≥60歲者90例。經(jīng)病理學(xué)診斷證實(shí),所有胃癌標(biāo)本的組織學(xué)類型均為腺癌,其中高、中分化腺癌49例,低分化腺癌83例。根據(jù)胃癌病理類型(Borrmann分型)將標(biāo)本分為4組：1組,隆起型(I型)3例；2組,局限潰瘍型(Ⅱ型).101例；3組,浸潤潰瘍型(Ⅲ型),26例；4組,彌漫浸潤型(Ⅳ型),2例。腫瘤位于胃底部者13例、位于胃體部者38例、位于胃竇／幽門部者81例。腫瘤長徑5cm者77例、≥5cm者55例。有淋巴結(jié)轉(zhuǎn)移者79例,無淋巴結(jié)轉(zhuǎn)移者53例；發(fā)生遠(yuǎn)處轉(zhuǎn)移者14例,無遠(yuǎn)處轉(zhuǎn)移者118例。腫瘤浸潤深度依據(jù)第7版胃癌TNM分期標(biāo)準(zhǔn)進(jìn)行判定,其中,T1/T2：2l例,T3/T4：111例(見表1)。本實(shí)驗(yàn)所納入患者術(shù)前均未曾接受化療、放療等輔助治療措施。采用免疫組織化學(xué)方法(PV9000兩步法)分別對132例胃癌組織中ErbB2與Ebpl蛋白的表達(dá)水平進(jìn)行測定。結(jié)果胃癌組織中ErbB2蛋白過表達(dá)陽性率為38.64%, Ebpl蛋白過表達(dá)陽性率為25.76%。統(tǒng)計分析顯示,與ErbB2過表達(dá)密切相關(guān)的臨床病理特征包括胃癌病理類型、分化程度、浸潤深度、淋巴結(jié)轉(zhuǎn)移及遠(yuǎn)處轉(zhuǎn)移(p0.05),而無論性別、年齡、民族、腫瘤大小、腫瘤位置如何變化,ErbB2過表達(dá)水平都無統(tǒng)計學(xué)差異(P0.05)；與Ebp1過表達(dá)密切相關(guān)的臨床病理特征包括胃癌病理類型、分化程度、淋巴結(jié)轉(zhuǎn)移及遠(yuǎn)處轉(zhuǎn)移(P0.05),而無論性別、年齡、民族、腫瘤大小、腫瘤位。置及浸潤深度如何變化,Ebp1過表達(dá)水平都無統(tǒng)計學(xué)差異(P0.05)。等級相關(guān)檢驗(yàn)提示ErbB2與Ebp1在胃癌組織中的表達(dá)呈正相關(guān)關(guān)系(P0.05)。結(jié)論1.ErbB2與Ebp1蛋白表達(dá)的聯(lián)合檢測有助于對胃癌惡性程度及侵襲、轉(zhuǎn)移傾向的判斷。2.ErbB2與Ebp1蛋白在胃癌組織中的表達(dá)呈正相關(guān)關(guān)系,二者可能協(xié)同促進(jìn)胃癌的發(fā)生、發(fā)展,Ebpl作為胃癌ErbB2靶向治療的輔助生物學(xué)靶點(diǎn)可能具有一定的意義。
[Abstract]:Objective to detect the expression of ErbB2 and Ebpl protein in gastric carcinoma by immunohistochemical method (PV9000 two-step method) and to analyze the relationship between the expression of ErbB2 and Ebp1 protein and the clinicopathological characteristics of gastric cancer and the correlation between the expression of ErbB2 and Ebp1 protein in gastric cancer tissue. Methods from January 2011 to November 2013, 132 specimens of gastric cancer were collected from the affiliated Hospital of Yanbian University, including 97 males and 35 females: 85 Han and 47 Korean. The average age was 63.03 鹵9.06 years. 42 cases were aged 60 years and 90 cases were over 60 years old. The histologic types of all gastric cancer specimens were confirmed by pathology. Among them, 49 cases were well differentiated adenocarcinoma, 83 cases were poorly differentiated adenocarcinoma. According to the pathological type of gastric cancer (Borrmann classification), the specimens were divided into 4 groups: 1 group, 3 cases of bulge type (type I), 3 cases of protuberance type (type I), 3 cases of localized ulcer type (type 鈪，

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