發(fā)布時(shí)間：2018-08-07 17:03

【摘要】：目的篩選出在膀胱癌組織中特異性表達(dá)的長(zhǎng)鏈非編碼RNA(lncRNA)及其共表達(dá)mRNA,并進(jìn)行初步驗(yàn)證及功能預(yù)測(cè)。方法采用lncRNA v4.0芯片篩選4對(duì)膀胱癌和癌旁組織中l(wèi)ncRNA及其共表達(dá)mRNA的差異表達(dá)譜,通過(guò)聚類(lèi)分析比較二者表達(dá)差異;采用實(shí)時(shí)熒光定量PCR(qRT-PCR)法對(duì)5個(gè)異常調(diào)節(jié)的lncRNA(RP11-359E19.2、AL928768.3、AC002519.6、RP11-79H23.3、AK021804)和4個(gè)共表達(dá)的mRNA(HRAS、VEGFA、ITGB1、DNMT3B)進(jìn)行驗(yàn)證。同時(shí),對(duì)lncRNA共表達(dá)的mRNA進(jìn)行GO及KEGG pathway富集分析。結(jié)果差異表達(dá)的lncRNA共4 155條,其中2 045條高表達(dá)、2 110條低表達(dá);與lncRNA共表達(dá)的mRNA共4 416條,其中2 472條高表達(dá)、1 944條低表達(dá)。|FC|≥10的lncRNA 345條,包括127條高表達(dá)和218條低表達(dá)。RP11-436F21.1和H19是上調(diào)最明顯的lncRNA。|FC|≥10的共表達(dá)mRNA有75條,其中57條上調(diào)、18條下調(diào)。與癌旁組織相比,膀胱癌組織5種lncRNA中RP11-359E19.2表達(dá)上調(diào),AL928768.3、AC002519.6、RP11-79H23.3及AK021804表達(dá)下調(diào)(P均0.05);4種共表達(dá)的mRNA中HRAS、VEGFA、ITGB1和DNMT3B表達(dá)均上調(diào)(P均0.05);qRT-PCR結(jié)果與芯片結(jié)果一致。GO分析顯示,上調(diào)的共表達(dá)mRNA主要參與細(xì)胞代謝和有絲分裂的生物學(xué)過(guò)程,下調(diào)的共表達(dá)mRNAs主要參與免疫系統(tǒng)的刺激反應(yīng)和免疫應(yīng)答;KEGG pathway富集分析顯示,10個(gè)通路與上調(diào)或下調(diào)的共表達(dá)mRNA有關(guān),其中在上調(diào)的共表達(dá)mRNA中,p53信號(hào)通路和膀胱癌的富集度最高,在下調(diào)的共表達(dá)mRNA中細(xì)胞因子-因子受體相互作用富集度最高。結(jié)論成功篩選出膀胱癌特異表達(dá)的lncRNA及其共表達(dá)mRNA,這些特異表達(dá)的lncRNA及共表達(dá)mRNA在膀胱癌的發(fā)生、發(fā)展和轉(zhuǎn)移中發(fā)揮重要的生物學(xué)作用,有望成為膀胱癌新的標(biāo)志物。
[Abstract]:Objective to screen the long chain noncoding RNA (lncRNA) and its coexpression mRNAs specifically expressed in bladder cancer tissues, and to carry out preliminary verification and functional prediction. Methods lncRNA v4.0 microarray was used to screen the differential expression profiles of lncRNA and its coexpression mRNA in bladder cancer and adjacent tissues, and the difference between them was compared by cluster analysis. Five abnormal regulated lncRNA (RP11-359E19.2HAL928768.3) and four co-expressed mRNA (HRAS-VEGFAITGB1DNMT3B) were tested by real-time fluorescence quantitative PCR (qRT-PCR). At the same time, the mRNA co-expressed by lncRNA were analyzed by go and KEGG pathway enrichment. Results there were 4 155 differentially expressed lncRNA, 2 045 high expression and 2 110 low expression, 4 416 mRNA coexpressed with lncRNA, 2 472 high expression and 1 944 low expression, 345 lncRNA with FC 鈮，

本文編號(hào)：2170733